钙离子荧光探针Fluo-8H, AM 货号21090-AAT Bioquest荧光染料

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品,欢迎访问AAT Bioquest荧光染料官网了解更多信息。

钙离子荧光探针Fluo-8H, AM

钙离子荧光探针Fluo-8H, AM

钙离子荧光探针Fluo-8H, AM    货号21090 货号 21090 存储条件 在零下15度以下保存, 避免光照
规格 1 mg 价格 3924
Ex (nm) 495 Em (nm) 516
分子量 1074.98 溶剂 DMSO
产品详细介绍

简要概述

产品基本信息

货号:21090

产品名称:钙离子荧光探针Fluo-8H, AM

规格:1mg

储存条件:-15℃避光防潮

保质期:24个月

 

产品物理化学光谱特性

分子量:1074.98

溶剂:水

激发波长(nm):494

发射波长(nm):517

 

适用仪器


荧光显微镜  
激发: FITC
发射: FITC
推荐孔板: 黑色透明
荧光酶标仪  
激发: 490nm
发射: 525nm
cutoff: 515nm
推荐孔板: 黑色透明
读取模式: 底读模式

 

产品介绍

钙离子荧光探针Fluo-8H, AM是美国AAT Bioquest生产的钙离子荧光探针,钙测量对于许多生物学研究至关重要。在结合Ca2 +后显示光谱响应的荧光探针使研究人员能够使用荧光显微镜,流式细胞仪,荧光光谱和荧光酶标仪来研究细胞内游离Ca2 +浓度的变化。在可见光激发钙指示剂中,Fluo-3和Fluo-4最常用。但是,Fluo-3 AM和Fluo-4 AM在酯酶水解后在活细胞中仅适度发荧光,并且需要苛刻的细胞加载条件才能最大化其细胞钙反应。开发Fluo-8®染料可改善细胞负载和钙响应,同时保持便捷的Fluo-3和Fluo-4光谱波长(在〜490 nm处具有最大激发和在〜520 nm处具有最大发射)。Fluo-8®AM仅需要室温,而Fluo-3 AM和Fluo-4 AM需要37℃的细胞负载。此外,Fluo-8®的亮度是Fluo-4 AM的2倍,是Fluo-3 AM的4倍。AAT Bioquest提供了一套我们的出色Fluo-8®试剂,具有不同的钙结合亲和力(Fluo-8®:Kd = 389 nM; Fluo-8H:Kd = 232 nM; Fluo-8L:Kd = 1.86 µM; Fluo-8FF :Kd = 10 µM)。我们还提供多种包装尺寸以满足您的特殊需求,例如1毫克;10×50 µg;20×50 µg;HTS包装,不收取额外包装费用。Kd = 232 nM;Fluo-8L:Kd = 1.86 µM;Fluo-8FF:Kd = 10 µM)。金畔生物是AAT Bioquest的中国代理商,为您提供最优质的钙离子荧光探针Fluo-8H, AM。 

点击查看光谱

点击查看实验方案

钙离子篇:时间轴式讲解应用于钙离子检测的探针

 

试剂应用文献

AMPA receptors in the synapse turnover by monomer diffusion
Authors: 
Morise, Jyoji and Suzuki, Kenichi GN and Kitagawa, Ayaka and Wakazono, Yoshihiko and Takamiya, Kogo and Tsunoyama, Taka A and Nemoto, Yuri L and Takematsu, Hiromu and Kusumi, Akihiro and Oka, Shogo
Journal: 
Nature communications (2019): 1–18

Cryo-EM Studies of TMEM16F Calcium-Activated Ion Channel Suggest Features Important for Lipid Scrambling
Authors: Feng, Shengjie and Dang, Shangyu and Han, Tina Wei and Ye, Wenlei and Jin, Peng and Cheng, Tong and Li, Junrui and Jan, Yuh Nung and Jan, Lily Yeh and Cheng, Yifan
Journal: Cell Reports (2019): 567–579

Discrimination of Dormant and Active Hematopoietic Stem Cells by G0 Marker Reveals Dormancy Regulation by Cytoplasmic Calcium
Authors: Fukushima, Tsuyoshi and Tanaka, Yosuke and Hamey, Fiona K and Chang, Chih-Hsiang and Oki, Toshihiko and Asada, Shuhei and Hayashi, Yasutaka and Fujino, Takeshi and Yonezawa, Taishi and Takeda, Reina and others
Journal: Cell Reports (2019): 4144–4158

Ketamine Increases Proliferation of Human iPSC-Derived Neuronal Progenitor Cells via Insulin-Like Growth Factor 2 and Independent of the NMDA Receptor
Authors: Grossert, Aless and ra and Mehrjardi, Narges Zare and Bailey, Sarah J and Lindsay, Mark A and Hescheler, Jürgen and Saric, Tomo and Teusch, Nicole
Journal: Cells (2019): 1139

MRGPRX4 is a bile acid receptor for human cholestatic itch
Authors: Yu, Huasheng and Zhao, Tianjun and Liu, Simin and Wu, Qinxue and Johnson, Omar and Wu, Zhaofa and Zhuang, Zihao and Shi, Yaocheng and Peng, Luxin and He, Renxi and others
Journal: eLife (2019): e48431

P2Y6 signaling in alveolar macrophages prevents leukotriene-dependent type 2 allergic lung inflammation
Authors: Nagai, Jun and Balestrieri, Barbara and Fanning, Laura B and Kyin, Timothy and Cirka, Haley and Lin, Junrui and Idzko, Marco and Zech, Andreas and Kim, Edy Y and Brennan, Patrick J and others
Journal: The Journal of clinical investigation (2019)

Hyperglycaemia disrupts conducted vasodilation in the resistance vasculature of db/db mice
Authors: Lemmey, Hamish AL and Ye, Xi and Ding, Hong C and Triggle, Christopher R and Garland, Christopher J and Dora, Kim A
Journal: Vascular pharmacology (2018): 29–35

Methionine and valine activate the mammalian target of rapamycin complex 1 pathway through heterodimeric amino acid taste receptor (TAS1R1/TAS1R3) and intracellular Ca2+ in bovine mammary epithelial cells
Authors: Zhou, Y and Zhou, Z and Peng, J and Loor, Juan J
Journal: Journal of dairy science (2018): 11354–11363

TRPA1-dependent reversible opening of tight junction by natural compounds with an $alpha$, $beta$-unsaturated moiety and capsaicin
Authors: Kanda, Yusuke and Yamasaki, Youhei and Sasaki-Yamaguchi, Yoshie and Ida-Koga, Noriko and Kamisuki, Shinji and Sugawara, Fumio and Nagumo, Yoko and Usui, Takeo
Journal: Scientific reports (2018): 1–13

A new electro-optical approach for conductance measurement: an assay for the study of drugs acting on ligand-gated ion channels
Authors: Menegon, A and Pitassi, S and Mazzocchi, N and Redaelli, L and Rizzetto, R and Roll and JF and Poli, C and Imberti, M and Lanati, A and Grohovaz, F
Journal: Scientific Reports (2017)

Altered spontaneous calcium signaling of in situ chondrocytes in human osteoarthritic cartilage
Authors: Gong, Xiaoyuan and Xie, Wenbin and Wang, Bin and Gu, Lingchuan and Wang, Fuyou and Ren, Xiang and Chen, Cheng and Yang, Liu
Journal: Scientific reports (2017): 17093

Bystander effects elicited by single-cell photo-oxidative blue-light stimulation in retinal pigment epithelium cell networks
Authors: Ishii, Masaaki and Rohrer, Bärbel
Journal: Cell Death Discovery (2017): 16071

Bystander effects elicited by single-cell photo-oxidative blue-light stimulation in retinal pigment epithelium cell networks
Authors: Ishii, Masaaki and Rohrer, Bärbel
Journal: Cell Death Discovery (2017): 16071

High-throughput screen detects calcium signaling dysfunction in typical sporadic autism spectrum disorder
Authors: Schmunk, Galina and Nguyen, Rachel L and Ferguson, David L and Kumar, Kenny and Parker, Ian and Gargus, J Jay
Journal: Scientific Reports (2017): 40740

 

参考文献

A Distinct Role of the Autonomic Nervous System in Modulating the Function of Lymphatic Vessels under Physiological and Tumor-Draining Conditions
Authors: Samia B Bachmann, Denise Gsponer, Javier A Montoya-Zegarra, Martin Schneider, Felix Scholkmann, Carlotta Tacconi, Simon F Noerrelykke, Steven T Proulx, Michael Detmar
Journal: Cell reports (2019): 3305–3314

Electrode-free nanopore sensing by DiffusiOptoPhysiology
Authors: Yuqin Wang, Yu Wang, Xiaoyu Du, Shuanghong Yan, Panke Zhang, Hong-Yuan Chen, Shuo Huang
Journal: Science Advances (2019): eaar3309

Passive and parallel microfluidic formation of droplet interface bilayers (DIBs) for measurement of leakage of small molecules through artificial phospholipid membranes
Authors: Magdalena A Czekalska, Tomasz S Kaminski, Karol Makuch, Piotr Garstecki
Journal: Sensors and Actuators B: Chemical (2019)

Development of micro mechanical device having two-dimensional array of micro chambers for cell stretching
Authors: K Minami, T Hayashi, K Sato, T Nakahara
Journal: Biomedical microdevices (2018): 10

Spatiotemporal magnetic fields enhance cytosolic Ca 2+ levels and induce actin polymerization via activation of voltage-gated sodium channels in skeletal muscle cells
Authors: Mónica Rubio Ayala, Tatiana Syrovets, Susanne Hafner, Vitalii Zablotskii, Alexandr Dejneka, Thomas Simmet
Journal: Biomaterials (2018)

2-OMe-lysophosphatidylcholine analogues are GPR119 ligands and activate insulin secretion from βTC-3 pancreatic cells: Evaluation of structure-dependent biological activity
Authors: Anna Drzazga, Agata Sowińska, Agnieszka Krzemińska, Andrzej Okruszek, Piotr Paneth, Maria Koziolkiewicz, Edyta Gendaszewska-Darmach
Journal: Biochimica et Biophysica Acta (BBA)-Molecular and Cell Biology of Lipids (2017)

A new electro-optical approach for conductance measurement: an assay for the study of drugs acting on ligand-gated ion channels
Authors: A Menegon, S Pitassi, N Mazzocchi, L Redaelli, R Rizzetto, JF Rolland, C Poli, M Imberti, A Lanati, F Grohovaz
Journal: Scientific Reports (2017)

Altered spontaneous calcium signaling of in situ chondrocytes in human osteoarthritic cartilage
Authors: Xiaoyuan Gong, Wenbin Xie, Bin Wang, Lingchuan Gu, Fuyou Wang, Xiang Ren, Cheng Chen, Liu Yang
Journal: Scientific reports (2017): 17093

Analysis of Ca2+ response of osteocyte network by three-dimensional time-lapse imaging in living bone
Authors: Tomoyo Tanaka, Mitsuhiro Hoshijima, Junko Sunaga, Takashi Nishida, Mana Hashimoto, Naoya Odagaki, Ryuta Osumi, Taiji Aadachi, Hiroshi Kamioka
Journal: Journal of Bone and Mineral Metabolism (2017): 1–10

Aryl-and alkyl-phosphorus-containing flame retardants induced mitochondrial impairment and cell death in Chinese hamster ovary (CHO-k1) cells
Authors: Chao Huang, Na Li, Shengwu Yuan, Xiaoya Ji, Mei Ma, Kaifeng Rao, Zijian Wang
Journal: Environmental Pollution (2017): 775–786

 

相关产品

产品名称 货号
钙离子荧光探针Cal-520 , AM Cat#21130
钙离子荧光探针Fluo-8, AM Cat#21080
新型钙离子荧光探针Calbryte 520, AM *细胞渗透性* Cat#20650

说明书
钙离子荧光探针Fluo-8H, AM.pdf