2-Aminoethoxypropargyl ddATP

	货号	17084	存储条件	在零下15度以下保存, 避免光照
	规格	1 umoles	价格	11988
	Ex (nm)		Em (nm)
	分子量	659.24	溶剂	DMF
	产品详细介绍

简要概述

产品基本信息

货号：17084

产品名称：2-Aminoethoxypropargyl ddATP

规格：1 umoles

储存条件：-15℃避光防潮

保质期：24个月

产品物理化学光谱特性

分子量：659.24

外观：液体

溶剂：DMF

激发波长(nm)：N/A

发射波长(nm)：N/A

产品介绍

Sanger法是DNA测序中最早最靠谱的方法之一，DNA由四种脱氧核苷酸三磷酸（dNTP）合成。将每个新核苷酸添加到最后的dNTP的3′-OH基团中。可以将二脱氧胸苷三磷酸（ddTTPs）添加到正在生长的DNA链中，但是当它出现时，链延长会停止，因为下一个要连接的核苷酸没有3′-OH。待测序的DNA被制备成单链。该模板DNA带有大量dATP，dGTP，dCTP和dTTP的混合物。加入四种双脱氧核苷酸（ddATP，ddGTP，ddCTP和ddTTP）的混合物，每种混合物均以限量存在，并分别用发不同颜色的荧光的“标签​​”标记。因为所有四个正常核苷酸都存在，所以链延伸正常进行，直到偶然地DNA聚合酶插入ddNTP（而不是正常dNTP）。如果正常核苷酸与双脱氧形式的比率足够高，则在插入ddNTP之前，某些DNA链将成功添加数百个核苷酸，从而终止该过程。在孵育期结束时，片段的长度从最长到最短分离，一个核苷酸之间的差异足以使该链与下一较短和下一较长链分开。当被激光束照射时，四个DDNTP中的每一个都会发出不同的荧光，可以通过自动扫描仪打印输出序列。这些ddATP，ddGTP，ddCTP和ddTTP胺衍生物是开发Sanger测序试剂的重要组成部分。金畔生物是AAT Bioquest的中国代理商，为您提供最优质的2-Aminoethoxypropargyl ddATP。 

参考文献

Polyadenylated sequencing primers enable complete readability of PCR amplicons analyzed by dideoxynucleotide sequencing
Authors: Beranek, M., Drastikova, M., Petera, J.
Journal: Acta Medica (Hradec Kralove) (2012): 160-4

UV-induced bond modifications in thymine and thymine dideoxynucleotide: structural elucidation of isomers by differential mobility mass spectrometry
Authors: St-Jacques, A., Anichina, J., Schneider, B. B., Covey, T. R., Bohme, D. K.
Journal: Anal Chem (2010): 6163-7

Analysis of processivity of mungbean dideoxynucleotide-sensitive DNA polymerase and detection of the activity and expression of the enzyme in the meristematic and meiotic tissues and following DNA damaging agent
Authors: Roy, S., Choudhury, S. R., Sengupta, D. N.
Journal: Arch Biochem Biophys (2008): 55-65

A dideoxynucleotide-sensitive DNA polymerase activity characterized from endoreduplicating cells of mungbean (Vigna radiata L.) during ontogeny of cotyledons
Authors: Roy, S., Sarkar, S. N., Singh, S. K., Sengupta, D. N.
Journal: FEBS J (2007): 2005-23

Mechanism-based suppression of dideoxynucleotide resistance by K65R human immunodeficiency virus reverse transcriptase using an alpha-boranophosphate nucleoside analogue
Authors: Selmi, B., Boretto, J., Sarfati, S. R., Guerreiro, C., Canard, B.
Journal: J Biol Chem (2001): 48466-72

Synthesis of the first ferrocene-labeled dideoxynucleotide and its use for 3′-redox end-labeling of 5′-modified single-stranded oligonucleotides
Authors: Anne, A., Blanc, B., Moiroux, J.
Journal: Bioconjug Chem (2001): 396-405

Improving dideoxynucleotide-triphosphate utilisation by the hyper-thermophilic DNA polymerase from the archaeon Pyrococcus furiosus
Authors: Evans, S. J., Fogg, M. J., Mamone, A., Davis, M., Pearl, L. H., Connolly, B. A.
Journal: Nucleic Acids Res (2000): 1059-66

Structure-based design of Taq DNA polymerases with improved properties of dideoxynucleotide incorporation
Authors: Li, Y., Mitaxov, V., Waksman, G.
Journal: Proc Natl Acad Sci U S A (1999): 9491-6

Characterization of the native and recombinant catalytic subunit of human DNA polymerase gamma: identification of residues critical for exonuclease activity and dideoxynucleotide sensitivity
Authors: Longley, M. J., Ropp, P. A., Lim, S. E., Copel and W. C.
Journal: Biochemistry (1998): 10529-39

Comparative performance of high-density oligonucleotide sequencing and dideoxynucleotide sequencing of HIV type 1 pol from clinical samples
Authors: Gunthard, H. F., Wong, J. K., Ignacio, C. C., Havlir, D. V., Richman, D. D.
Journal: AIDS Res Hum Retroviruses (1998): 869-76