钙蓝素,AM CAS 168482-84-6 货号22007-AAT Bioquest荧光染料

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钙蓝素,AM CAS 168482-84-6

钙蓝素,AM CAS 168482-84-6

钙蓝素,AM CAS 168482-84-6    货号22007 货号 22007 存储条件 在零下15度以下保存, 避免光照
规格 1 mg 价格 1008
Ex (nm) 354 Em (nm) 441
分子量 465.41 溶剂 DMSO
产品详细介绍

简要概述

钙蓝素,AM是美国AAT Bioquest生产的细胞渗透性钙黄绿素,它是钙黄绿素的细胞渗透型。进入活细胞后,弱荧光钙黄绿素AM被水解成钙黄绿素,其激发/发射最大值与DAPI,Hoechst和AMCA相似。与典型的绿色和红色荧光团(如FITC,TMR和德克萨斯红)的这种特殊光谱分离为多路复用实验提供了额外的选择。因为钙黄绿素AM本身是荧光的,所以可能需要适当的过滤器组和额外的洗涤步骤以使背景荧光最小化。我们的钙测定缓冲液可用于有效洗涤细胞外钙黄绿素。金畔生物是AAT Bioquest的中国代理商,为您提供最优质的钙蓝素,AM。 

点击查看光谱

点击查看实验方案

 

适用仪器


流式细胞仪  
激发: 350 nm or 405 nm激光
发射: 450/40nm滤波片
推荐孔板: Pacific Blue通道
荧光显微镜  
激发: DAPI
发射: DAPI
推荐孔板: 黑色透明
荧光酶标仪  
激发: 360nm
发射: 450nm
cutoff: 420nm
推荐孔板: 黑色孔板
读取模式: 底读模式

产品说明书

操作步骤

1.准备HHBS缓冲液,10%Pluronic®F-127溶液和25 mM Probenecid溶液。

2.在高质量无水DMSO中制备2 mM至5 mM Calcein Blue,AM * CAS 168482-84-6 *储备液。
2.1卡介素蓝量,AM * CAS 168482-84-6 *使用量:1毫克
2.2所需浓度:2 mM
2.3在合适的容器中,将1mg钙黄绿素,AM * CAS 168482-84-6 *与1074.32μL无水DMSO混合。
 

3.用含有10μM钙黄绿素的HHBS制备2X工作溶液,AM * CAS 168482-84-6 * 4,0.08%Pluronic®F-127和2 mM丙磺舒。
3.1Calcein Blue的最终孔内浓度,AM * CAS 168482-84-6 *:5μM
3.2Pluronic®F-127的最终孔内浓度:0.04%
3.3最终孔内浓度的丙磺舒:1mM
3.4在合适的容器中混合16μL钙黄绿素,AM * CAS 168482-84-6 *,25.6μL10%Pluronic®F-127和256μL25mM Probenecid。然后,添加HHBS或您选择的缓冲液,直到体积为3.2 mL。
注意:对于大多数细胞系,我们建议最终浓度为Calcein Blue,AM * CAS 168482-84-6 *为4至5μM。
注意:推荐的Pluronic F-127井浓度最终为0.02%至0.04%。
注意:推荐的最终浓度为1至2.5 mM的Probenecid。
 

4.将100μL染料工作溶液加入已经含有100μL培养基的所需孔中。
4.1此步骤将染料工作溶液从2X稀释至1X,并将每种组分的最终浓度调整为以下:5μM钙黄绿素,AM * CAS 168482-84-6 *,0.04%Pluronic®F-127,1 mM丙磺舒。

 

5.孵育染料
5.1将染料加载板在细胞培养箱中孵育20-60分钟。
5.2将染料加载板在室温下孵育30分钟。
 

6.用1.0 mM Probenecid准备HHBS缓冲液(或您选择的缓冲液)。
6.1在合适的容器中加入160μL的25mM丙磺舒。 接下来,添加HHBS或您选择的缓冲液,直到体积为4 mL。
 

7.用HHBS缓冲液或您选择的缓冲液替换染料工作溶液,使用1.0 mM Probenecid。
7.1首先,从所需孔中除去200μL染料工作溶液和培养基。
7.2在相同的孔中加入200μL含有1.0mM丙磺舒的HHBS(或您选择的缓冲液)。
 

8.运行实验
8.1为您的样品添加所需的处理。
8.2以Ex / Em = 360/445 nm运行实验。

 

参考文献

Functional imaging of neuronal activity of auditory cortex by using Cal-520 in anesthetized and awake mice
Authors: Jingcheng Li, Jianxiong Zhang, Meng Wang, Junxia Pan, Xiaowei Chen, Xiang Liao
Journal: Biomedical Optics Express (2017): 2599–2610

NINJ2–A novel regulator of endothelial inflammation and activation
Authors: Jingjing Wang, Jingjing Fa, Pengyun Wang, Xinzhen Jia, Huixin Peng, Jing Chen, Yifan Wang, Chenhui Wang, Qiuyun Chen, Xin Tu
Journal: Cellular Signalling (2017)

Erythropoietin Stimulates Endothelial Progenitor Cells to Induce Endothelialization in an Aneurysm Neck After Coil Embolization by Modulating Vascular Endothelial Growth Factor
Authors: Peixi Liu, Yingjie Zhou, Qingzhu An, Yaying Song, Xi Chen, Guo-Yuan Yang, Wei Zhu
Journal: MEDICINE (2016): 1–8

Flexible Endoscopic Spray Application of Respiratory Epithelial Cells as Platform Technology to Apply Cells in Tubular Organs
Authors: Anja Lena Thiebes, Manuel Armin Reddemann, Johannes Palmer, Reinhold Kneer, Stefan Jockenhoevel, Christian Gabriel Cornelissen
Journal: Tissue Engineering Part C: Methods (2016): 322–331

Influence of hypothermia and subsequent rewarming upon leukocyte-endothelial interactions and expression of Junctional-Adhesion-Molecules A and B
Authors: Nicolai V Bogert, Isabella Werner, Angela Kornberger, Patrick Meybohm, Anton Moritz, Till Keller, Ulrich A Stock, Andres Beiras-Fernandez
Journal: Scientific reports (2016)

Inhibition of ABC transport proteins by oil sands process affected water
Authors: Hattan A Alharbi, David MV Saunders, Ahmed Al-Mousa, Jane Alcorn, Alberto S Pereira, Jonathan W Martin, John P Giesy, Steve B Wiseman
Journal: Aquatic Toxicology (2016): 81–88

Rapid generation of collagen-based microtissues to study cell–matrix interactions
Authors: Marie-Elena Brett, Alexandra L Crampton, David K Wood
Journal: Technology (2016): 1–8

Toxicokinetics and toxicodynamics of chlorpyrifos is altered in embryos of Japanese medaka exposed to oil sands process-affected water: evidence for inhibition of P-glycoprotein
Authors: Hattan A Alharbi, Jane Alcorn, Ahmed Al-Mousa, John P Giesy, Steve B Wiseman
Journal: Journal of Applied Toxicology (2016)

Spraying respiratory epithelial cells to coat tissue-engineered constructs
Authors: Anja Lena Thiebes, Stefanie Albers, Christian Klopsch, Stefan Jockenhoevel, Christian G Cornelissen
Journal: BioResearch open access (2015): 278–287

The efficient hemostatic effect of Antarctic krill chitosan is related to its hydration property
Authors: Shuai Wu, Zhuoyao Huang, Jianhui Yue, Di Liu, Ting Wang, Pierre Ezanno, Changshun Ruan, Xiaoli Zhao, William W Lu, Haobo Pan
Journal: Carbohydrate polymers (2015): 295–303

 

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说明书
钙蓝素,AM CAS 168482-84-6.pdf