Screen Quest 人类孤啡肽受体 Ga16 CHO (NOP-Ga16)细胞株

简要概述

产品基本信息

货号：38100

产品名称：Screen Quest 人类孤啡肽受体 Ga16 CHO (NOP-Ga16)细胞株

规格：Each

产品介绍

超过60％的已知G蛋白偶联受体（GPCR）通过Gq以外的途径发出信号，导致细胞内钙增加。随着基因组学揭示出更多的G蛋白偶联受体靶标，这种趋势持续增加。 Screen Quest 细胞系能够研究通常不通过细胞内钙偶联的GPCR。 Screen Quest 细胞系基于一系列G蛋白嵌合体，包括混杂的G蛋白Gα16。嵌合体由Gq蛋白复合物的α亚基组成，该复合物的5个羧基末端氨基酸已被其他G蛋白之一（Gαs，Gαi，Gαo或Gαz）取代。这些氨基酸负责受体与其G蛋白的偶联。这些嵌合体与特定的非Gq偶联受体共表达可能会在受体刺激后导致细胞内钙信号的产生。 Screen Quest CHO-Ga16-NOP细胞系是用混杂G蛋白Gα16和三种人类阿片受体之一伤害感受器（也称为ORL-1）稳定转染的CHO-K1细胞。细胞中组成型表达的Gα16蛋白使通常通过cAMP途径发挥作用的该受体与Gq信号转导和动员的细胞内钙偶联。可以使用钙敏感染料（例如Calbryte 520 AM，Cal-520 AM，Fluo-8 AM或Fluo-4 AM）和相应的免洗钙试剂盒检测特定配体对这些细胞中阿片样物质/伤害感受器的激活。该细胞系已成功用于药物发现和筛选环境中，以研究通常不通过细胞内钙偶联的GPCR。它已与FLIPR，FDSS系统一起有效使用。金畔生物是AAT Bioquest的中国代理商，为您提供最优质的Screen Quest 人类孤啡肽受体 Ga16 CHO (NOP-Ga16)细胞株。 

图示

图1.用Cal-520®，AM（Cat＃21130）在CHO-Ga16-NOP细胞中测量了伤害感受肽刺激的钙反应。 将CHO-Ga16-NOP细胞以60,000个细胞/ 100 µL /孔在Costar黑色96孔板中接种过夜。 在含有20 mM Hepes缓冲液（HHBS）的Hanks中，将等体积（100 µL）的10 µMCal-520®AM和2 mM丙磺舒在37°C下孵育1小时。 用具有1 mM丙磺舒的HHBS代替Cal-520®AM上样溶液。 通过FlexStation（分子设备）添加了Nociceptin，以达到最终指示的浓度。

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