O-糖苷酶 & 神经氨酸苷酶套装 货 号 #E0540S-NEB酶试剂 New England Biolabs

上海金畔生物科技有限公司代理New England Biolabs(NEB)酶试剂全线产品,欢迎访问官网了解更多产品信息和订购。

产品资料 – 糖生物学与蛋白质工具 – 糖苷外切酶

O-糖苷酶 & 神经氨酸苷酶套装                              收藏

O-糖苷酶 & 神经氨酸苷酶套装                 货   号                  #E0540S O-糖苷酶 & 神经氨酸苷酶套装                 货   号                  #E0540S O-糖苷酶 & 神经氨酸苷酶套装                 货   号                  #E0540S O-糖苷酶 & 神经氨酸苷酶套装                 货   号                  #E0540S O-糖苷酶 & 神经氨酸苷酶套装                 货   号                  #E0540S

货 号
规 格
价 格(元)

1 bundle


  • isoschizomers     |
  • compatible ends     | 
  • single letter code


1 set of this bundle includes 2,000,000 units of O-Glycosidase and 2,000 units of Neuraminidase.

O-Glycosidase,   also known as Endo-α-N-Acetylgalactosaminidase,   catalyzes the removal of

Core 1 and Core 3 O-linked disaccharides from glycoproteins. 

Neuraminidase is the common name for Acetyl-neuraminyl  hydrolase ( Sialidase ).   This Neura-minidase catalyzes the hydrolysis of α2-3, α2-6, α2-
8 linked N-acetyl-neuraminic  acid  residues

from glycoproteins and oligosaccharides. 

O-糖苷酶 & 神经氨酸苷酶套装                 货   号                  #E0540S


O-糖苷酶 & 神经氨酸苷酶套装                 货   号                  #E0540S

Product Source

O-Glycosidase is cloned from Enterococcus faecalis and expressed in E.coli (1). Neuraminidase

is cloned from Clostridium perfringens (2) and overexpressed in E. coli (3).

Reagents Supplied

The following reagents are supplied with this product:

O-糖苷酶 & 神经氨酸苷酶套装                 货   号                  #E0540S

Properties and Usage

Unit Definition

One unit of O-Glycosidase is defined as the amount of enzyme required to remove 0.68 nmol of O-linked disaccharide from 5 mg of Neuraminidase digested, non-denatured fetuin in 1 hour at 37°C in a total reaction volume of 100 µl (1 unit of both O-Glycosidase and PNGase F will remove equivalent molar amounts of O-linked disaccharides and N-linked oligosaccharides, respectively).

Non-denaturing Unit Definition of O-Glycosidase:
Two  fold  serial  dilutions  of  O-Glycosidase  are added to a reaction mixture of 5 mg  of Neura-minidase  digested  fetuin with 1 X GlycoBuffer 2. The  reaction  is  then  incubated at 37°C for 1 hour. O-linked disaccharide carbohydrates are determined by Morgan and Elson Assay (4).
Note: Under  denaturing  conditions  the  enzyme  activity  is  increased  two-fold. This observation  is substrate dependent.

Unit Definition of Neuraminidase:
One unit of Neuraminidase is defined as the amount of enzyme required to cleave > 95% of the   terminal α-Neu5Ac from 1 nmol Neu5Acα2-3Galβ1-3GlcNAcβ1-3Galβ1-4Glc-7-amino-4-methyl-   coumarin (AMC), in 5 minutes at 37°C in a total reaction volume of 10 µl.

1X Glycoprotein Denaturing Buffer
0.5% SDS
40 mM DTT

1X NP-40
1% NP-40 in MilliQ-H2O

Reaction Conditions

1X GlycoBuffer 2
Incubate at 37°C

1X GlycoBuffer 2:
50 mM sodium phosphate
pH 7.5 @ 25°C

Heat Inactivation


Related Products

 Companion Products

α2-3,6,8 Neuraminidase


PNGase F

PNGase F (Glycerol-free)

Endo H

Endo Hf


1.Since O-Glycosidase is inhibited by SDS, it is essential to have NP-40 in the reaction mixture. It is not known why this non-ionic detergent counteracts the SDS inhibition at the present  time. Double digest with Endo H must have NP-40 present (NP-40 does not inhibit Endo H).

2.To deglycosylate a native glycoprotein, longer incubation time as well as more enzyme may be required.


 1.Koutsioulis, D., Landry, D. and Guthrie, E.P. (2008). Glycobiology. 18, 799-805.

2.Roggentin, P. et al. (1988). FEBS Lett. 238, 31-34.

3.Guan, C. unpublished observations. New England Biolabs.

4.Morgan, W.T.J. and Elson, L.A. (1934). Biochem. J. 28, 988-995.