ReadiCleave ROXtra AML-NHS酯

	货号	7002	存储条件	在零下15度以下保存, 避免光照
	规格	1 mg	价格	4836
	Ex (nm)		Em (nm)
	分子量	1379.58	溶剂	DMSO
	产品详细介绍

简要概述

产品基本信息

货号：7002

产品名称：ReadiCleave ROXtra AML-NHS酯

规格：1 mg

储存条件：-15℃避光防潮

保质期：12个月

产品物理化学光谱特性

分子量：1379.58

外观：固体

溶剂：DMSO

产品介绍

荧光法生物检测在灵敏度和便利性的方面具有很大的优势。许多生物分子可以很容易地用荧光标签标记，用于荧光成像和流式细胞仪分析。 然而，大多数现有的荧光标签用于永久性标记生物靶标，从中不能切割添加的荧光标签以进行进一步的下游分析，例如质谱分析。 AAT Bioquest的ReadiCleave 探针使荧光标签与生物靶标结合，可以在需要时从中去除附加的荧光标签。 此ReadiCleave ROXtra 包含一个叠氮基甲基化合物，可以用TCEP裂解该化合物，以从靶分子中除去ROXtra荧光团。 可以通过添加10 mM TCEP溶液（pH 7.5）并在65°C孵育1-5分钟来进行裂解。ROXtra是新研发的荧光团，具有与标准ROX染料相同的光谱，并显著提高了稳定性和水溶性。

参考文献

A public website for the automated assessment and validation of SARS-CoV-2 diagnostic PCR assays.
Authors: Li, Po-E and Myers Y Gutiérrez, Adán and Davenport, Karen and Flynn, Mark and Hu, Bin and Lo, Chien-Chi and Player Jackson, Elais and Shakya, Migun and Xu, Yan and Gans, Jason D and Chain, Patrick S G
Journal: Bioinformatics (Oxford, England) (2021): 1024-1025

A real-time multiplex PCR assay for detection of the causative agents of rat bite fever, Streptobacillus moniliformis and zoonoticStreptobacillus species.
Authors: Kelly, Aubree J and Ivey, Melissa L and Gulvik, Christopher A and Humrighouse, Ben W and McQuiston, John R
Journal: Diagnostic microbiology and infectious disease (2021): 115335

A simple quantitative PCR assay to determine TRAMP transgene zygosity.
Authors: Chen, Ruidong and Liang, Xin and Murray, Mollianne M and Karasik, Ellen and Han, Jenny J and Zhu, Ming and Foster, Barbara A and Frigo, Daniel E and Wang, Guocan
Journal: Prostate cancer and prostatic diseases (2021): 358-361

Accuracy and Performance Evaluation of Triplet Repeat Primed PCR as an Alternative to Conventional Diagnostic Methods for Fragile X Syndrome.
Authors: Gu, Hyunjung and Kim, Man Jin and Yang, Dahae and Song, Ji Yun and Cho, Sung Im and Park, Sung Sup and Seong, Moon-Woo
Journal: Annals of laboratory medicine (2021): 394-400

An effective analytical droplet digital PCR approach for identification and quantification of fur-bearing animal meat in raw and processed food.
Authors: Yu, Ning and Ren, Junan and Huang, Wensheng and Xing, Ranran and Deng, Tingting and Chen, Ying
Journal: Food chemistry (2021): 129525

Application of laser capture microdissection and PCR sequencing in the diagnosis of Coccidioides spp. infection: A case report and literature review in China.
Authors: Yang, Xinyu and Song, Yinggai and Liang, TianYu and Wang, Qiqi and Li, Ruoyu and Liu, Wei
Journal: Emerging microbes & infections (2021): 331-341

Application of newly developed SARS-CoV2 serology test along with real-time PCR for early detection in health care workers and on-time plasma donation.
Authors: Soltani-Zangbar, Mohammad Sadegh and Aghebati-Maleki, Leili and Hajivalili, Mahsa and Haji-Fatahaliha, Mostafa and Motavalli, Roza and Mahmoodpoor, Ata and Kafil, Hossein Samadi and Farhang, Sara and Pourakbari, Ramin and Jadidi-Niaragh, Farhad and Roshangar, Leila and Heris, Javad Ahmadian and Kamrani, Amin and Siahmansouri, Homayoon and Hosseini, Maryam and Miahipour, Abolfazl and Shareghi-Oskoue, Oldouz and Parhizkar, Forough and Yousefi, Mehdi
Journal: Gene reports (2021): 101140

Characterization of the adiponectin promoter + Cre recombinase insertion in the Tg(Adipoq-cre)1Evdr mouse by targeted locus amplification and droplet digital PCR.
Authors: Wong, Adrian M and Patel, Tushar P and Altman, Elizabeth K and Tugarinov, Nicol and Trivellin, Giampaolo and Yanovski, Jack A
Journal: Adipocyte (2021): 21-27

Comparative evaluation of a dual-target real-time RT-PCR assay for COVID-19 diagnosis and assessment of performance in pooled saliva and nasopharyngeal swab samples.
Authors: Yip, Cyril C Y and Leung, Kit-Hang and Ng, Anthony C K and Chan, Kwok-Hung and To, Kelvin K W and Chan, Jasper F W and Hung, Ivan F N and Cheng, Vincent C C and Sridhar, Siddharth
Journal: Expert review of molecular diagnostics (2021)

Comparison of the AdvanSure RV Plus Real-Time RT-PCR and Real-Q RV II Detection Assays for Respiratory Viruses.
Authors: Chung, Yoo Na and Yoo, In Young and Yun, Sun Ae and Kim, Ji-Youn and Lee, Nam Yong and Huh, Hee Jae
Journal: Annals of laboratory medicine (2021): 506-509

死细胞核染料EthD-2 [Ethidium Homodimer-2](停产)

	货号	17782	存储条件	在零下15度以下保存, 避免光照
	规格	1 mg	价格	0
	Ex (nm)		Em (nm)
	分子量	1292.71	溶剂	DMSO
	产品详细介绍

简要概述

产品基本信息

货号：17782

产品名称：死细胞核染料EthD-2 [Ethidium Homodimer-2]

规格：1mg

储存条件：-15℃避光防潮

保质期：12个月

产品物理化学光谱特性

分子量：1292.71

外观：固体

溶剂：DMSO

产品介绍

Ethidium Homodimer-2 (EthD-2) 通常用作细胞非渗透性活力指标。 它是一种高亲和力核酸染色剂，在与 DNA 结合之前呈微弱荧光，并发出红色荧光（激发/发射最大值 ~535/624）。 它通常与钙黄绿素 AM 一起使用。 通过用钙黄绿素乙酰氧基甲酯 (Calcein AM) 和 EthD-2 染色，可以很容易地评估细胞的活力。 活细胞（绿色，用 Calcein AM 染色）和死细胞（红色，用 EthD-2 染色）可以很容易地成像，甚至可以使用 ImageJ 程序从图像中量化。 它还可以与我们的其他细胞活力染料结合使用，例如卓越的 Calcein Ultra Blue、Calcein Ultra Green、Cytocalcein、Cytotrace 染料和 CytoTell 染料。金畔生物是AAT Bioquest的中国代理商，为您提供最优质的细胞核染料。 

参考文献

Iatrogenic Articular Cartilage Injury in Arthroscopic Hip and Knee Videos and the Potential for Cartilage Cell Death When Simulated in a Bovine Model.
Authors: Compton, Jocelyn and Slattery, Michael and Coleman, Mitchell and Westermann, Robert
Journal: Arthroscopy : the journal of arthroscopic & related surgery : official publication of the Arthroscopy Association of North America and the International Arthroscopy Association (2020): 2114-2121

In Vitro Effect of Vancomycin on the Viability of Articular Chondrocytes.
Authors: Shaw, K Aaron and Eichinger, Josef K and Nadig, Nischal and Parada, Stephen A
Journal: Journal of orthopaedic trauma (2018): 148-153

Chondrocyte Viability After a Simulated Blast Exposure.
Authors: Shaw, K Aaron and Johnson, Peter C and Williams, David and Zumbrun, Steven D and Topolski, Richard and Cameron, Craig D
Journal: Military medicine (2017): e1941-e1947

Why Do Osteochondral Allografts Survive? Comparative Analysis of Cartilage Biochemical Properties Unveils a Molecular Basis for Durability.
Authors: Ding, Lei and Zampogna, Biagio and Vasta, Sebastiano and Jang, Kee Woong and De Caro, Francesca and Martin, James A and Amendola, Annunziato
Journal: The American journal of sports medicine (2015): 2459-68

DNA counterstaining for methylation and hydroxymethylation immunostaining in bovine zygotes.
Authors: Heras, Sonia and Forier, Katrien and Rombouts, Koen and Braeckmans, Kevin and Van Soom, Ann
Journal: Analytical biochemistry (2014): 14-6

Pretreatment of therapeutic cells with poly(ADP-ribose) polymerase inhibitor enhances their efficacy in an in vitro model of cell-based therapy in myocardial infarct.
Authors: Szepes, Mónika and Janicsek, Zsófia and Benkő, Zsolt and Cselenyák, Attila and Kiss, Levente
Journal: International journal of molecular medicine (2013): 26-32

Reliability of Hoechst 33342 staining under wide-field microscopy for evaluation of the nuclear status of living dog oocytes.
Authors: Chebrout, Martine and Adenot, Pierre-Gaël and Reynaud, Karine and Chastant-Maillard, Sylvie
Journal: Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada (2012): 483-92

Nuclear and cytoplasmic maturation of canine oocytes related to in vitro denudation.
Authors: Chebrout, M and de Lesegno, C Viaris and Reynaud, K and Chat, S and Chastant-Maillard, S
Journal: Reproduction in domestic animals = Zuchthygiene (2009): 243-6

C5 inhibitor rEV576 protects against neural injury in an in vitro mouse model of Miller Fisher syndrome.
Authors: Halstead, Susan K and Humphreys, Peter D and Zitman, Femke M P and Hamer, John and Plomp, Jaap J and Willison, Hugh J
Journal: Journal of the peripheral nervous system : JPNS (2008): 228-35

Continuous exposure to bisphenol A during in vitro follicular development induces meiotic abnormalities.
Authors: Lenie, Sandy and Cortvrindt, Rita and Eichenlaub-Ritter, Ursula and Smitz, Johan
Journal: Mutation research (2008): 71-81

TAQuest qPCR Master Mix with Helixyte Green *无 ROX*

	货号	17270	存储条件	在零下15度以下保存, 避免光照
	规格	1 mL	价格	1164
	Ex (nm)		Em (nm)
	分子量		溶剂	Water
	产品详细介绍

简要概述

产品基本信息

货号：17270

产品名称：TAQuest qPCR Master Mix with Helixyte Green *无 ROX*

规格：1ml

储存条件：-15℃避光防潮

保质期：12个月

产品物理化学光谱特性

溶剂：水

产品介绍

TAQuest qPCR Master Mix with Helixyte Green 是一种即用型 2X溶液，针对 qPCR 和 2 步 RT-qPCR 进行了优化。预混液在优化的 PCR 缓冲液中包含我们专有的 TAQuest 热启动 Taq DNA 聚合酶和 dNTP。您只需添加模板和目标引物即可进行所需的 PCR 反应。热启动 Taq DNA 聚合酶允许您在室温下设置 PCR 反应，从而最大限度地减少非特异性产物的形成。该酶与优化的缓冲液结合使用，可确保对所有样品类型（如基因组、质粒、病毒和 cDNA 模板）的 PCR 特异性和灵敏度。 Helixyte Green 嵌入染料无需使用序列特异性探针即可快速检测和分析 DNA。该预混液不含 ROX 参考染料。金畔生物是AAT Bioquest的中国代理商，为您提供最优质的TAQuest qPCR Master Mix with Helixyte Green *无 ROX*。

适用仪器

样品实验方案
注意 在室温下用 Helixyte Green *无 ROX* 解冻 TAQuest™ qPCR Master Mix。 使用前彻底涡旋 qPCR Master Mix。
1. 制备表 1 所示的下列反应混合物之一。
2. 轻轻涡旋混合试剂，然后短暂离心。
3. 在 qPCR 仪器中设置板并按表 2 所示操作。

表 1. 各反应每孔试剂组成

表 2. 热循环参数

参考文献

A SYBR Green I-based real-time polymerase chain reaction assay for detection and quantification of canine bufavirus.
Authors: Wang, Yong and Sun, Jianfei and Guo, Xu and Li, Wei and Zhang, Da and Liu, Guangqing and Zhou, Tianhong and Li, Yongdong
Journal: Molecular and cellular probes (2021): 101762

A duplex SYBR green I-based real-time polymerase chain reaction assay for concurrent detection of feline parvovirus and feline coronavirus.
Authors: Sun, Liting and Xu, Zhiqing and Wu, Junhuang and Cui, Yongqiu and Guo, Xu and Xu, Fazhi and Li, Yongdong and Wang, Yong
Journal: Journal of virological methods (2021): 114294

A new SYBR Green real-time PCR to detect SARS-CoV-2.
Authors: Marinowic, D R and Zanirati, G and Rodrigues, F V F and Grahl, M V C and Alcará, A M and Machado, D C and Da Costa, J C
Journal: Scientific reports (2021): 2224

A novel duplex SYBR Green real-time PCR with melting curve analysis method for beef adulteration detection.
Authors: Li, Jiapeng and Wei, Yixuan and Li, Jinchun and Liu, Ruixi and Xu, Suigen and Xiong, Suyue and Guo, Ya and Qiao, Xiaoling and Wang, Shouwei
Journal: Food chemistry (2021): 127932

A rapid and low-cost protocol for the detection of B.1.1.7 lineage of SARS-CoV-2 by using SYBR Green-based RT-qPCR.
Authors: Abdel Sater, Fadil and Younes, Mahmoud and Nassar, Hassan and Nguewa, Paul and Hamze, Kassem
Journal: Molecular biology reports (2021): 7243-7249

Design and characterization of a SYBR Green I-based melting curve method for investigation of HER2I655V polymorphism in breast cancer.
Authors: Desriani and Azamris and Ghaissani, Shabrina S and Kinanti, Senja R and Warisman, Muhammad A and Fitria, N
Journal: Journal, genetic engineering & biotechnology (2021): 6

Development and Validation of a SYBR Green Real Time PCR Protocol for Detection and Quantification of Nervous Necrosis Virus (NNV) Using Different Standards.
Authors: Olveira, José G and Souto, Sandra and Bandín, Isabel and Dopazo, Carlos P
Journal: Animals : an open access journal from MDPI (2021)

Development and application of SYBR Green Ⅰ real-time quantitative reverse transcription PCR assay for detection of swine Getah virus.
Authors: Xia, Yin-He and Shi, Zi-Cong and Wang, Xin-Wei and Li, Yong-Tao and Wang, Zeng and Chang, Hong-Tao and Liu, Hong-Ying and Chen, Lu and Wang, Chuan-Qing and Yang, Xia
Journal: Molecular and cellular probes (2021): 101730

Development of New PCR Assay with SYBR Green I for Detection of Mycoplasma, Acholeplasma, and Ureaplasma sp. in Cell Cultures.
Authors: Krzysztoń-Russjan, Jolanta and Chudziak, Jakub and Bednarek, Małgorzata and Anuszewska, Elżbieta Lidia
Journal: Diagnostics (Basel, Switzerland) (2021)

Development of SYBR Green I-based polymerase chain reaction for feline bocavirus 1 detection.
Authors: Wang, Yong and Li, Wei and Guo, Xu and Zhang, Da and Sun, Jianfei and Fu, Ziteng and Liu, Guangqing and Li, Yongdong and Jiang, Shudong
Journal: 3 Biotech (2021): 61