Hampton晶体删选试剂盒

Hampton晶体删选试剂

1.Screens 晶体删选试剂盒

产品名称 货号 用途备注
Index Kit HR2-144 用于生物大分子结晶
CrystalScreen Kit HR2-110 用于蛋白质,核酸水溶性小分子结晶
Crystal Screen 2 Kit HR2-112
Pegrx 1 HR2-082 用于生物大分子结晶
Pegrx 2 HR2-084 用于生物大分子结晶
Peg/Ion Screen Kit HR2-126 用于生物大分子结晶
Peg/Ion 2 Screen™ HR2-098 用于生物大分子结晶
Saltrx Kit HR2-108 用于生物大分子结晶
Membfac Kit HR2-114 用于膜蛋白和溶解度有限的样品结晶
Crystal Screen Lite HR2-128 用于蛋白质,缩氨酸,核酸的结晶
Natrix Kit HR2-116 用于核酸和蛋白核酸复合物的结晶
Crystal Screen Cyro Kit HR2-122 用于蛋白质,可溶性缩氨酸,核酸和水溶性小分子的初次低温结晶
Nucleic Acid Mini Screen Kit HR2-118 用于核酸片段的结晶
Low Ionic Strength Screen HR2-120 用于完整单抗,单抗片段以及在低离子浓度下溶解度较低的样品结晶
Silver Bullet & Silver Bullet Bio HR2-078 用于生物大分子的初次和二次结晶
HR2-080
Peg/Tacsimate Ph5.8 Crystallization Reagent HR2-090
Peg/Tacsimate Ph 6.8 Crystallization Reagent HR2-092
Peg/Tacsimate Ph 7.8 Crystallization Reagent HR2-094
Additive Screen™ HR2-428 利用样品间和样品溶剂间相互作用来优化晶体生长和改变样品溶解度
产品名称 货号 用途备注
Detergent Screen 1 HR2-410 改善疏水作用引起的非特异性聚集
Detergent Screen 2 HR2-411
Detergent Screen 3 HR2-412
Detergent Screen HR2-408
Heavy Atom Screen Pt HR2-442 Heavy Atoms For Multiple Isomorphous Replacement
Heavy Atom Screen Au HR2-444
Heavy Atom Screen Hg HR2-446
Heavy Atom Screen M1 HR2-448
Heavy Atom Screen M2 HR2-450
I3c Phasing Kit HR3-133

2.Custom Shop Crystallization Reagents 结晶试剂盒中单独的试剂

  1. Optimize™ Reagents晶体生长条件优化试剂

⑴ Optimize™ – Polymers聚合体

⑵ Optimize™- Organics (Volatile) 挥发性有机物

⑶ Optimize™- Organics (Non-Volatile)非挥发性的有机物

⑷ Optimize™- Salts 盐类

⑸ Optimize™- Buffers缓冲液

⑹ Optimize™- Solubilizing Agents (NDSB) 溶解试剂NDSB

⑺ Optimize™ – Reducing Agent还原剂-TCEP氢氯化物

⑻ Optimize™ – Cryoprotectants

⑼ Optimize™ – Oils用于结晶的油类

⑽ Optimize™ – Silica Hydrogel硅胶

⑾ Optimize™ – LM Agarose低熔点琼脂糖
5.Crystallization Plates, Hardware & Accessories结晶耗材
⑴ 24 Well Crystallization Plates 24孔晶体板-分悬滴和坐滴两种
⑵ 48 Well Crystallization Plates 48孔晶体板
⑶ 72 Well Crystallization Plates72孔晶体板
⑷ 96 Well Crystallization Plates 96孔晶体板等

Hampton 蛋白结晶向导管Wizard Tube

Wizard Tube 
The Wizard™ Classic line of random sparse matrix screens is designed to increase your probability of producing crystals during the coarse screening phase when crystallizing biological macromolecules (proteins, nucleic acids, peptides, and combinations thereof).
The Wizard Classic reagents are proven to be a highly effective starting point in the screening of biological macromolecules. The Wizard Classic formulations include a large range of crystallants, buffers, and salts covering a broad range of crystallization space at pH levels from pH 4.5 to pH 10.5.Choose from Wizard Classic 1, 2, 3, or 4 non-overlapping formulations in matrix blocks or tubes.

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Wizard Cubic LCP
The tools in the Wizard Cubic LCP (lipidic cubic phase) Kit enable researchers to prepare LCP-type crystallizations by hand. Ideal for low-protein experiments: effective protein volume for a single crystallization experiment is about 80 nanoliters. Wizard Cubic LCP Kit tools work especially well when traditional methods have failed to yield crystals. Lipidic cubic phase has worked well for the crystallization of 7TM membrane proteins (proteins with seven transmembrane helices). Four out of six GPCRs (G-protein coupled receptors), an important membrane protein class, and several microbial 7TM proteins have been crystallized using the LCP approach.

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CRYO (I、II)
The Wizard Cryo™ line of random sparse matrix screens is designed for scientists who want to avoid the additional step of optimizing a cryoprotectant condition. Every Wizard Cryo formulation flash-freezes to a clear, amorphous glass in liquid nitrogen or in a cryo-stream at 100K. Crystals can be frozen directly from their growth drops, avoiding the additional step of pre-equilibration with an artificial cryo-condition that can damage the crystal. Eleven different cryocrystallants and sparing use of glycerol ensures a broad sampling of possible cryo conditions. Choose from Wizard Cryo 1 or 2 formulations in tubes or Wizard Cryo 1 and 2 together in a 96-well matrix block.

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Hampton 24孔坐滴板 Cryschem Plate

Hampton 24孔坐滴板 Cryschem Plate

Applications

 Sitting drop crystallization

  Heavy atom soaks

  Seeding

Features

Optically clear, concave sitting drop post
Cryschem Plate – Larger footprint and round reagent reservoir
Cryschem M – Microplate footprint with round reagent reservoir
Cryschem S – Microplate footprint with square reagent reservoir

Compatible with Formulatrix Formulator

Smooth, flat top for easy seal
Each plate supplied with a cover

Materials

  Optically clear polystyrene

 Description

The Cryschem Plate is a 24 well sitting drop plate that is sealed with clear sealing tape. The polystyrene plate is supplied with a raised cover so the plate can also be sealed with vacuum grease and plain glass or plastic cover slides (22 mm diameter). The stackable, 24 well plates have distortion-free, flat bottom wells and a concave sitting drop post in the center of the well for concentric equilibration of the drop with the reagent. Each plate is individually sealed. The top of the plate and each of the 24 wells is a single, flat plane for smooth and easy seal application. Well spacing provides for a large seal area between wells to prevent cross contamination and evaporation.

上海金畔生物作为Hampton蛋白结晶产品的代理商,竭诚为您服务,欢迎新老客户咨询。

Cryschem Plate is large plate footprint with circular reagent reservoir. Cryschem plate approximate size: 15.1 cm x 10.6 cm x 2.2 cm. Approximate well size: 1.6 cm x 1.5 cm. Typical fill volume: 500 to 1,000 µl. Well capacity: 1.5 ml. Maximum drop volume on post: 40 µl. Recommended seal: HR3-511 Crystal Clear Sealing Tape (1.88 inch x 43.7 yard roll, with cutter) or HR4-511 Crystal Clear Sealing Tape (1.88 inch x 60 yard roll, without cutter).

Cryschem M Plate is microplate footprint with circular reagent reservoir. Cryschem M microplate approximate size: 128 mm x 86 mm x 14 mm. Approximate circular reservoir size: 15 mm wide x 9 mm deep. Typical reservoir volume: 500 µl. Well capacity: 1.1 ml. Maximum drop volume on post: 30 µl. Recommended seal: HR3-609 Crystal Clear Sealing Film (100 pack), HR4-521 ClearSeal Film (100 pack) or HR4-506 Crystal Clear Sealing Tape (3 inch x 55 yard roll, without cutter). HR4-508 Crystal Clear Sealing Tape (0.75 inch x 650 inch, with cutter) seals a single row at a time.

Cryschem S Plate is microplate footprint with square reagent reservoir. Cryschem S microplate approximate size: 128 mm x 86 mm x 14 mm. Approximate square reservoir size: 15 mm wide x 9 mm deep. Typical reservoir volume: 500 µl. Well capacity: 1.1 ml. Maximum drop volume on post: 30 µl. Recommended seal: HR3-609 Crystal Clear Sealing Film (100 pack), HR4-521 ClearSeal Film (100 pack) or HR4-506 Crystal Clear Sealing Tape (3 inch x 55 yard roll, without cutter). HR4-508 Crystal Clear Sealing Tape (0.75 inch x 650 inch, with cutter) seals a single row at a time.

Cryschem Plate

Cryschem plate

CAT NO NAME DESCRIPTION
HR3-158 Cryschem Plate 24 plate case
HR3-160 Cryschem Plate 100 plate case
HR1-002 Cryschem M Plate (round reagent reservoir) 50 plate case
HR3-308 Cryschem S Plate (square reagent reservoir) 50 plate case

Sigma代理  Abcam代理  CST代理  Santa Cruz代理  Biolegend代理 ebioscience代理  Invitrogen代理   millipore代理  BD流式抗体代理   GeneTe x抗体代理  Novus抗体代理   R&D代理 Biovison代理  Jackson代理  MBL抗体代理  ProSpec抗体代理  Bethyl抗体代理  Antibody Revolution抗体代理  Torrey Pines Biolabs代理  Amresco代理  MPbio代理  Laysan bio代理  NANOCS代理  Avanti代理  wako代理  lumiprobe代理(活性荧光染料)   NEB酶代理  Roche酶代理  Toyobo酶代理   USP代理  EP代理  Dr代理  TRC代理  TCI代理  Reagecon代理 Megazyme代理 Hampton代理(蛋白结晶) whatman代理(滤膜滤纸) GE代理(蛋白纯化) Corning康宁代理  Axygen代理 Falcon代理  NISSUI日水代理 Himedia代理 OXOID代理  BD培养基代理 Ludger代理(糖蛋白分析产品)  Eppendorf代理  Labnet代理  标准品代理 抗体代理 酶试剂代理  培养基代理 耗材代理 Elisa试剂盒代理 。

Hampton Siliconized Glass Cover Slides

Hampton Siliconized Glass Cover Slides

ApplicationsHanging, sitting or sandwich drop crystallization
FeaturesHigh purity of glassSiliconized, hydrophobic glass surface0.22 mm & 0.96 mm glass thicknessThicker glass slides preserve temperature longer12 mm, 18 mm, & 22 mm glass diameterCircle & squareUV Compatible (>85% transmission)
12 x 0.22 mm Circle
18 x 0.22 mm Circle
22 x 0.22 mm Circle & Square

Description
Siliconized glass cover slides allow a droplet to be suspended in a position which provides near optimal conditions for vapor diffusion with the surrounding reservoir solution. These siliconized glass cover slides provide a consistent, high quality finish for crystallization experiments. The hydrophobic surface produces a drop which “stands well” and does not flatten on the glass. The siliconized surface prevents the adhesion of crystals and precipitate onto the glass surface. Use Dow Corning Vacuum Grease (HR3-510), Dow Corning 7 Release Compound (HR3-508), or immersion oil to seal cover slide to plate. Available in 12, 18 or 22 mm diameter circles and 22 mm diameter squares. Available in 0.22 mm or 0.96 mm glass thickness. The 0.96 mm thick slides are virtually unbreakable during crystallization handling.

The following slides are UV transmissable (>85%):
HR3-277 and HR3-279 – 12 mm x 0.22 mm Siliconized circle cover slides
HR3-231 and HR3-233 – 22 mm x 0.22 mm Siliconized circle cover slides
HR3-239 and HR3-241 – 18 mm x 0.22 mm Siliconized circle cover slides
HR3-215 and HR3-217 – 22 mm x 0.22 mm Siliconized square cover slides

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Plain Glass Cover Slides
ApplicationsHanging, sitting or sandwich drop crystallization
Features
0.22 mm glass thickness
12 mm glass diameter
Hydrophilic glass surface
Circle & squareDescription
Plain, non-siliconized, hydrophilic glass cover slides. Useful for hanging drop, sitting drop, and sandwich drop vapor diffusion crystallization methods. The hydrophilic surface creates a drop which is more flat than a drop on a siliconized surface. This offers enhanced imaging for very small drops. Use Dow Corning® Vacuum Grease (HR3-510), Dow Corning® 7 Release Compound (HR3-508), or immersion oil to seal cover slide to plate.

HR3-209T 12 mm Plain circle cover slides for Tecan robot 5 oz case (~2,400 slides)

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HR3-209T 12 mm Plain circle cover slides for Tecan robot 5 oz case (~2,400 slides)
PEN-VAC Kit
ApplicationsHolding and manipulating small, flat surfaces such as glass cover slides
FeaturesSelf-contained vacuumNo power neededCompactDescription
PEN-VAC is a great tool for handling small, flat surfaced objects such as plain and siliconized cover slides, as well as plastic slides. Simply depress the side button, touch the tip to the object to be lifted and release the button. PEN-VAC creates a totally self-contained vacuum that lifts up to 50 grams for up to one minute. Less than an ounce, the lightweight aluminum body fits in a pocket. No power supply needed. The kit includes one PEN-VAC with 5 3/4″ blue body, (3) angled and (3) straight probes with (2) each 1/8”, 1/4”, 3/8” diameter blue silicone vacuum cups . It is packaged in a clear plastic storage case.

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HR3-251  PEN-VAC Kit

Hampton 0.1毫米50号玻璃毛细管

Hampton 0.1毫米50号玻璃毛细管

货号:HR6-104
包装:25 pack

  • 英文名:0.1 mm Glass Number 50 Capillary
  • 品牌:Hampton
Hampton 0.1毫米50号玻璃毛细管

Hampton代理–金畔生物

硼硅玻璃50号毛细管壁厚0.01毫米,全长80毫米+ / – 5毫米。提供外径从0.1毫米到2毫米的毛细管,用于X射线数据收集,晶体密度测量和晶体生长实验。毛细管可以用蜡,环氧树脂等密封材料封闭,防止水分和气体。

应用

X射线数据采集

液-液扩散结晶

凝胶针状结晶

特征

50号硼硅玻璃

壁厚 10微米

描述

硼硅玻璃50号毛细管一端呈漏斗形,另一端封闭,壁厚0.01毫米,全长80毫米+/-5毫米,外径从0.1毫米到2毫米。可嵌入,盛放,储存小分子和生物大分子晶体用于X射线数据收集,晶体密度测量和晶体生长实验。毛细管可以用蜡,环氧树脂和其它密封材料密封,防止水分和气体。

请参照“线性吸收系数µcm-1表“,以决定适合的玻璃/石英玻璃毛细管。本表展示的是X射线数据收集时的辐射吸收量。

0.1mm至2.0mm毛细管开口端的基础尺寸为:3.0+/-0.15mm外径 x 0.13+/-0.10 mm壁厚,直径为在毛细管封闭端40毫米处的测量值。

 

货号

产品说明

公差

HR6-104

Size: 0.1 mm – 25 pack

0.1 mm -0.075 / +0.075 mm

HR6-106

Size: 0.2 mm – 25 pack

0.2 mm -0.050 / +0.050 mm

HR6-108

Size: 0.3 mm – 25 pack

0.3 mm -0.050 / +0.100 mm

HR6-110

Size: 0.4 mm – 25 pack

0.4 mm -0.100 / +0.100 mm

HR6-112

Size: 0.5 mm – 25 pack

0.5 mm -0.100 / +0.100 mm

HR6-114

Size: 0.6 mm – 25 pack

0.6 mm -0.100 / +0.100 mm

HR6-116

Size: 0.7 mm – 25 pack

0.7 mm -0.150 / +0.150 mm

HR6-118

Size: 0.8 mm – 25 pack

0.8 mm -0.150 / +0.150 mm

HR6-120

Size: 0.9 mm – 25 pack

0.9 mm -0.150 / +0.150 mm

HR6-122

Size: 1.0 mm – 25 pack

1.0 mm -0.150 / +0.250 mm

HR6-124

Size: 1.5 mm – 25 pack

1.5 mm -0.250 / +0.250 mm

HR6-126

Size: 2.0 mm – 25 pack

2.0 mm -0.250 / +0.250 mm

Hampton蛋白结晶柱 CrystalCap™ SPINE HT

Hampton CrystalCap™ SPINE HT

Applications

Cryocrystallography   低温结晶学

Features

SPINE style sample mount for cryocrystallography
CrystalCap SPINE HT attaches magnetically to CrystalCap Vial and Magnetic Goniometer Base     CrystalCap SPINE HT磁性地附着到CrystalCap小瓶和磁测角仪底座
Hollowed out design compatible with SPINE style grippers, auto mounters and sample handlers
2D alphanumeric coding for sample tracking & management
Bar coded, color coded, alphanumeric, magnetic cap
Color coded CryoLoop size
 Description
The CrystalCap SPINE HT is a magnetic sample mount (also known as a cap, pin or goniometer base) designed for cryocrystallography systems that accept SPINE style caps. The CrystalCap SPINE HT attaches to a magnetic CrystalCap SPINE Vial and magnetic goniometer base. The tip of the CrystalCap SPINE HT accepts a Mounted CryoLoop™ or MicroTube™ fitted with a CryoLoop™.The CrystalCap SPINE Vial is a 1.8 ml cryo vial featuring two small vents and is compatible with the CrystalCap SPINE HT. A ring magnet is molded into the open end of the vial so that when the cap is positioned in the vial, the ring magnet holds the cap on the vial during cryogenic storage. The CrystalCap SPINE Vial features chamfered edges for enhanced cap positioning as well as a magnetic alloy bottom for stability. The HR8-114 CrystalCap SPINE Vial does have a magnet on the bottom of the vial.The hollowed out design of the CrystalCap SPINE HT is compatible with SPINE style grippers, auto mounters and sample handlers.The CrystalCap SPINE HT features a two dimensional (2D) alphanumeric 16 x 16 data matrix code on the underside of the cap. Each cap is also color coded for CryoLoop size.

CrystalCap SPINE without 2D code/printing (HR8-094) is the CrystalCap SPINE HT cap only, without 2D code, without color code, without white white background on bottom of cap and no alphanumeric side labeling; cap only, without Mounted CryoLoop.

Note: Caps with Mounted CryoLoops are sold without vials. Vials available separately.

Color Coded Cap…………CryoLoop Size
Red…………………………….0.025-0.05 mm
Green…………………………….0.05-0.1 mm
Yellow……………………………..0.1-0.2 mm
Blue………………………………..0.2-0.3 mm
Blue/Red………………………….0.3-0.4 mm
Green/Red………………………..0.4-0.5 mm
Yellow/Red………………………..0.5-0.7 mm
Yellow/Green……………………..0.7-1.0 mm

Compatible with the following Synchrotron Radiation Beamlines

North & South America
• The Advanced Light Source, Berkeley, California ALS 4.2.2, ALS 11.3.1, ALS 12.2.2, ALS 12.3.2
• The Advanced Photon Source, Argonne, Illinois APS 14-BM-C BioCARS, APS 14-ID-B BioCARS, APS 21-ID-D LS-CAT, APS 21-ID-E LS-CAT, APS 21-ID-F LS-CAT, APS 21-ID-G LS-CAT, APS 23-ID-B GA/CA, APS 23-ID-D GA/CA, APS 24-ID-E NE-CAT, APS 31-ID LR-CAT
• Center for Advanced Microstructures and Devices, Baton Rouge, Louisiana CAMD GCPCC
• Cornell High Energy, Synchrotron Source, Ithaca, New York CHESS A1, CHESS F1
• The Brazilian Synchrotron Light Laboratory, Sao Paulo, Brazil LNLS D03B-MX1, LNLS W01B-MX2
• National Synchrotron Light Source, Upton, New York NSLS X3B, NSLS X4A, NSLS X4C, NSLS X6A, NSLS X12B, NSLS X12C, NSLS X25, NSLS X26C, NSLS X29

Europe
• Cerdanyola del Vallés, Spain ALBA XALOC
• Berlin Electron Storage Ring Company for Synchrotron Radiation, Berlin, Germany BESSY 14.1, BESSY 14.2, BESSY 14.3
• Diamond Light Source, Didcot, Oxfordshire, England DIAMOND I02, DIAMOND I03, DIAMOND I04, DIAMOND I04.1, DIAMOND I24
• Elettra Sincrotrone Trieste, Trieste, Italy ELETTRA 5.2R
• European Molecular Biology Laboratory, Hamburg, Germany EMBL/DESY P13, EMBL/DESY P14
• European Synchrotron Radiation Facility, Grenoble, France ESRF BM14, ESRF BM30A, ID30A-1/MASSIF-1,
ID30A-3/MASSIF-3, ESRF ID23-1, ESRF ID23-2, ESRF ID29
• Max-Lab, Lund University, Sweden MAX II I911-2, MAX II I911-3
• Swiss Light Source at Paul Scherer Institut, Switzerland SLS X06DA-PXIII, SLS X06SA-PXI, SLS X10SA-PXII
• SOLEIL, Saint-Aubin, France SOLEIL PROXIMA1, SOLEIL PROXIMA2

Asia & Australia
• Shanghai Synchrotron Radiation Facility, Shanghai, China SSRF BL17U1
• Beijing Synchrotron Radiation Facility, Beijing, China BSRF 1W2B, BSRF 3W1A
• Super Photon ring-8 GeV, Japan SPRING-8 BL12B2, SPRING-8 BL24XU, SPRING-8 BL26B1, SPRING-8 BL26B2, SPRING-8 BL32B2, SPRING-8 BL32XU, SPRING-8 BL38B1, SPRING-8 BL41XU, SPRING-8 BL44B2, SPRING-8 BL44XU

CrystalCap™ SPINE HT

CrystalCap SPINE HT

CrystalCap™ SPINE HT

CrystalCap SPINE HT

CrystalCap™ SPINE HT

CrystalCap SPINE HT

CAT NO NAME DESCRIPTION
HR8-118 CrystalCap SPINE HT 0.025-0.05 mm CryoLoop, without Vial – 30 pack
HR8-120 CrystalCap SPINE HT 0.05-0.1 mm CryoLoop, without Vial – 30 pack
HR8-122 CrystalCap SPINE HT 0.1-0.2 mm CryoLoop, without Vial – 30 pack
HR8-124 CrystalCap SPINE HT 0.2-0.3 mm CryoLoop, without Vial – 30 pack
HR8-126 CrystalCap SPINE HT 0.3-0.4 mm CryoLoop, without Vial – 30 pack
HR8-128 CrystalCap SPINE HT 0.4-0.5 mm CryoLoop, without Vial – 30 pack
HR8-130 CrystalCap SPINE HT 0.5-0.7mm CryoLoop, without Vial – 30 pack
HR8-132 CrystalCap SPINE HT 0.7-1.0 mm CryoLoop, without Vial – 30 pack
HR8-112 CrystalCap SPINE HT Cap only – 30 pack
HR8-116 CrystalCap SPINE HT Cap/Vial Cap/Vial only – 30 pack
HR8-094 CrystalCap SPINE without 2D code/printing Cap only – 30 pack
HR8-114 CrystalCap SPINE Vial Vial only – 30 pack

Hampton GRAS 5 • GRAS 6 Screens

Hampton GRAS 5 • GRAS 6 Screens

Applications

 GRAS reagent crystallization screen for proteins, including monoclonal antibodies, where Polyethylene glycol is the primary reagent, sampling pH 4.5 to 10

. Identify GRAS based reagents that promote crystallization of biotherapeutics for bioprocess, bioformulation, and continuous flow manufacturing applications

Features

Developed at Hampton Research
Generally Recognized As Safe reagent formulation
Samples pH 4.5 to 10; 8 unique buffers
Primary crystallization reagent:

GRAS 5: PEG 300, 400, MME 550, & 600
GRAS 6: PEG 1,000, MME 2,000, 3,350 & 4,000

 

Vapor diffusion, microbatch, free interface diffusion
Reagents soluble between 4°C and 30°C

Description

GRAS Screen™ 5 and 6 were developed by Hampton Research for the crystallization of proteins, including monoclonal antibodies. Each of the chemicals in GRAS Screen 5 and 6 has been used under one or more of the following categories. As (1) a Generally Recognized As Safe (GRAS) substance, (2) a pharmaceutical excipient, (3) a normal physiological constituent, (4) a metabolic byproduct, and/or (5) a Everything Added to Food in the United States (EAFUS) substance.

GRAS Screen 5 samples four low molecular weight Polyethylene glycols (300, 400, MME 550, and 600) at three concentrations versus eight unique buffers encompassing pH 4.5 to 10. GRAS Screen 5 is supplied in a 96 Deep Well block format and is compatible with robotic and multi-channel pipet liquid handling systems. GRAS Screen 5 is compatible with vapor diffusion, free interface diffusion, and microbatch crystallization methods. For research use only.

GRAS Screen 6 samples four medium molecular weight Polyethylene glycols (1,000, MME 2,000, 3,350, & 4,000) at three concentrations versus eight unique buffers encompassing pH 4.5 to 10. GRAS Screen 6 is supplied in a 96 Deep Well block format and is compatible with robotic and multi-channel pipet liquid handling systems. GRAS Screen 6 is compatible with vapor diffusion, free interface diffusion, and microbatch crystallization methods. For research use only.

Hampton 蛋白结晶试剂–Crystal Screen

Hampton 蛋白结晶试剂–Crystal Screen

Crystal Screen • Crystal Screen 2 • Crystal Screen HT

Applications

 Primary screen for proteins, soluble peptides, nucleic acids, & water soluble small molecules

 Sparse matrix additive screen

Features

 The original sparse matrix screen

 Sparse matrix formula efficiently samples salts, polymers, organics, & pH

 Proven effective with more than 1,000 biological macromolecules

 Tube or Deep Well block format

Description
The Crystal Screen and Crystal Screen 2 reagent kits are designed to provide a highly effective and rapid screening method for the crystallization of macromolecules. The screens are simple and practical for finding initial crystallization conditions. The initial crystallization conditions for more than 1,000 proteins, peptides, oligonucleotides, and small molecules have been determined using Crystal Screen.

A highly effective approach to overcome the exhaustive search for suitable crystallization conditions is the use of a sparse matrix method of trial conditions that is biased and selected from known crystallization conditions for macromolecules. The formulation utilized in Crystal Screen and Crystal Screen 2 evaluates 96 unique mixtures of pH, salts, polymers and organics, and their ability to promote crystal growth.

Crystal Screen contains 50 unique reagents, 10 ml each and is based on the sparse matrix formulation first described by Jancarik and Kim in 1991.

Crystal Screen 2, an extension of Crystal Screen, contains 48 unique reagents, 10 ml each and is based on the formulation first described by Cudney et al in 1994.

Crystal Screen HT contains 1 ml each of reagents 1-48 from Crystal Screen and all 48 reagents from Crystal Screen 2 in a single Deep Well block format.

Ready-to-use reagents are sterile filtered and formulated with ultra-pure Type 1 water, using the highest purity salts, polymers, organics and buffers. Individual reagents are available through the Hampton Research Custom Shop.

Crystal Screen • Crystal Screen 2 • Crystal Screen HT

Crystal Screen • Crystal Screen 2 • Crystal Screen HT

Crystals of a carbohydrate processing enzyme grown using Hampton Research Crystal Screen. Shirley Roberts, York Structural Biology Laboratory, York, United Kingdom.
Crystal Screen • Crystal Screen 2 • Crystal Screen HT

Crystal of zRFP574 red fluorescent protein grown using Hampton Research Crystal Screen 2. Pletneva et al. Acta Cryst. D62, 527-532 (2006). Nadya, Russian Academy of Science, Moscow, Russia.

CAT NO NAME DESCRIPTION
HR2-110 Crystal Screen 10 ml, tube format
HR2-112 Crystal Screen 2 10 ml, tube format
HR2-130 Crystal Screen HT 1 ml, Deep Well block format

Hampton蛋白结晶工具及仪器

Hampton蛋白结晶工具及仪器

CryoTong™ – Long
ApplicationsCrystal transfer under cryo temperature
FeaturesArtery clamp style closure30 seconds of cryo temperature180 mm length
Description
The CryoTong is a tool used to manually transfer a crystal mounted on a CrystalCap™ from liquid nitrogen to a magnetic base in a goniometer head positioned in a cryogenic stream, and then back to liquid nitrogen. The one-piece, compact CryoTong is available in two lengths. The long CryoTong is approximately 180 mm in overall length. The artery clamp style maintains the CryoTong in the closed position until the clamp is squeezed, which opens the opposing heads. The heads are non-magnetic stainless steel. The handle is magnetic stainless steel. The inside of the head is machined to closely surround the CrystalCap with loop and crystal in place. A small retaining lip is machined into the lower portion of the head to prevent the CrystalCap from slipping out of the CryoTong when the tool is in the closed position. The CryoTong can maintain the temperature of the crystal at -160°C for up to 30 seconds during room temperature crystal transfers. Choose the proper CryoTong size based upon the pin length required by the configuration of the x-ray data collection hardware used. The 18 mm CryoTong (HR5-112) is to be used with the CrystalCap HT and any other CrystalCap configured for 18 mm.HR5-114 Long CryoTong 18 mm, 180° features the head in alignment with the handle of the CryoTong.

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Vial Clamp™ – Straight
ApplicationsVial support and manipulation
FeaturesStraight end tipHemostat style closureDescription
The Vial Clamp™ – Straight is a chrome plated, hemostat style tool. It has a tip shaped to hold the storage vial of all the CrystalCap Systems™ straight when the clamp is closed. The clamp can be locked using the hemostat style closure. This clamp makes it easy to dip the storage vial into liquid nitrogen for crystal storage. The complete length of the clamp is straight, with an overall length of 195 mm. When the vial is placed in the clamp, the length of it is positioned perpendicular, 90° to the length of the clamp.
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CrystalWand™ Magnetic
ApplicationsCrystalCap Magnetic & CrystalCap Copper Magnetic handling toolDescription
The CrystalWand Magnetic is designed to be used exclusively with the CrystalCap Magnetic, CrystalCap Copper Magnetic, and CrystalCap HT systems during transfer of the caps from the vial to the goniometer head and from the goniometer to the vial. The 6 1/2” (165 mm) long chrome plated steel wand features a plastic housing enclosing a spring tensioned plunger that when depressed, moves a non-magnetic steel platform away from the magnet housed in the end of the wand. This causes the steel CrystalCap to detach from the wand and be placed readily into the vial or the CryoTong™. When the platform is retracted, the wand securely holds the steel CrystalCap Magnetic. The CrystalWand Magnetic 45° (McMiken Tool) offers the same functionality but is bent at a 45° angle at approximately 2.5 cm from the magnet end.HR4-729 CrystalWand Magnetic, StraightHR4-315 CrystalWand Magnetic, 45° (McMiken Tool)

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Vial Clamp™ – Curved
ApplicationsVial support & manipulation
FeaturesCurved end tipHemostat style closureDescription
The Vial Clamp – Curved is a chrome plated, hemostat style tool. It has a tip shaped to hold the storage vial of all the CrystalCap Systems at an angle when the clamp is closed. The clamp can be locked using the hemostat style closure. The end of the clamp, where the vial is held, is curved at either a 45°/135° or 110°/ 70° angle. When the vial is placed in the clamp, the length of it is positioned to the clamp at either of those same angles. The overall length of each clamp is the same, 195 mm.HR4-671 Vial Clamp – Curved 45°/135°HR4-672 Vial Clamp – Curved 110°/70°

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CrystalCap™ Holder
pplicationsCrystalCap support inside stainless steel dewars
FeaturesAdjustable heightSupports up to 8 CrystalCaps15 ml vial position for liquid propane500 and 1,000 ml versionsDescription
CrystalCap Holders are convenient stands for holding all CrystalCap™ systems in liquid nitrogen dewars during crystal cryogenic procedures. Available in two sizes. Both holders feature spring-clips to secure the CrystalCap Vials in the holder. Both stands have 3 inches of adjustable Z (height) which allows one to raise or lower the CrystalCaps to the appropriate height, depending upon the liquid nitrogen level in the dewar. Each holder is supplied with a socket driver for height adjustment.500 ml version holds five CrystalCaps, two 15 ml vials (for liquid propane procedures) and fits into the 500 ml dewar. 1,000 ml version holds eight CrystalCaps, two 15 ml vials (for liquid propane procedures) and fits into the 1,000 ml dewar.HR4-707 CrystalCap Holder – 500 ml
HR4-706  Replacement Height Adjustment Tool

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Hampton 膜蛋白结晶试剂 MembFac • Crystal Screen Lite • MembFac HT

Hampton  MembFac • Crystal Screen Lite • MembFac HT

Applications

Primary sparse matrix crystallization screen for membrane proteins and samples with limited solubility   膜蛋白和样品溶解度有限样品

Features

  Membrane protein sparse matrix screens

  Sparse matrix formula efficiently samples salts, polymers, organics and pH

  pH range 4.6 – 8.5

  Formulated for use with detergents

   Tube or Deep Well block format

   Crystal Screen Lite features a lower ionic strength than the original Crystal Screen

Description
MembFac is a highly effective sparse matrix screen specifically designed as a preliminary screen for the crystallization of membrane proteins. MembFac is based upon the highly effective biased sparse matrix methodology. The MembFac screen matrix is optimized for the crystallization of membrane proteins.

MembFac contains 48 unique reagent formulations. With this set of 48 conditions, numerous chemicals and chemical combinations are utilized, allowing one to evaluate a large variety of potential crystallization conditions. MembFac covers a broad pH range between 4 and 9.

The Rationale
The basic rationale of MembFac is to perform a sparse matrix screen while changing the detergent dimension because detergents play an important factor in the crystallization of membrane proteins. A MembFac screen is to be completed for each detergent. Detergent screens are available separately.

The Method
The membrane protein of interest is first isolated in the detergent which gives the highest stability and activity. The protein concentration should be approximately 10 to 20 mg/ml and the detergent concentration should be only slightly above the CMC. The sample is then set against MembFac, using all 48 solutions plus the screening detergent(s) of choice. The screening detergent concentration should be approximately one to three times the CMC. Hence, for each detergent screened, one would utilize approximately 1 to 2 mg of sample.

The MembFac protocol was used to crystallize membrane proteins including SQR, fumerate: quinone oxireductase, LHII, and LHCII.

Crystal Screen Lite is a sparse matrix of trial crystallization reagent conditions based upon the Crystal Screen™.3 The primary screen variables are salt, pH, and precipitant (salts, polymers, volatile organics, and non-volatile organics).2Crystal Screen Lite differs from the original Crystal Screen kit in that the primary precipitant reagents are one-half the concentration of that used in the original Crystal Screen formulation. The secondary salts, ions, and buffers remain at the original Crystal Screen concentration. Reducing the primary concentration of the primary precipitant results in a screen which is “more gentle” on the sample and typically produces much less precipitate conditions than the original Crystal Screen.

MembFac contains 48 unique reagents, 10 ml each.

MembFac HT contains 1 ml each of all 48 reagents from MembFac and reagents 1-48 from Crystal Screen Lite in a single Deep Well block format.

Ready-to-use reagents are sterile filtered and formulated with ultra-pure Type 1 water, using the highest purity salts, polymers, organics and buffers. Individual reagents are available through the Hampton Research Custom Shop.

CAT NO NAME DESCRIPTION
HR2-114 MembFac 10 ml, tube format
HR2-128 Crystal Screen Lite 10 ml, tube format
HR2-137 MembFac HT 1 ml, Deep Well block format