干细胞多能性评估PCR试剂盒 (Stem Cell Pluripotency Status Check Kit with PCR Primers)
The Pluripotency Check PCR Kit用来通过PCR方法监控在发育多能性方面有关键作用的基因,从而判断小鼠胚胎干细胞或是iPS是否真实具有干细胞多能性(pluripotency)。本产品覆盖16个基因,包括9个在真实干细胞中表达的基因和2个内参基因。客户可以灵活决定使用哪些基因进行实际检测。本产品可以鉴别关键基因的表达是内源性的(endogenous expression)还是外源性的(exogenous expression)。
■ Pluripotency Check PCR Primers
Primer set
Amplicon size
Primer set
Amplicon size
Oct3/4 (Total)
485 bp
Klf4
739 bp
Oct3/4 (Endogenous)
224 bp
Klf4 (Endogenous)
206 bp
Nanog (Total)
364 bp
Ecat1
164 bp
Nanog (Endogenous)
223 bp
ERas1
210 bp
Sox2 (Total)
193 bp
Esg1
376 bp
Sox2 (Endogenous)
297 bp
Rex1
287 bp
c-Myc (Total)
228 bp
Beta-Actin (Control)
603 bp
c-Myc (Endogenous)
170 bp
GAPDH (Control)
568 bp
■ 产品特点
Validate the pluripotent status of your stem cell or iPS cell culture using a simple PCR kit · 16 prevalidated PCR primer mixes · Complete kit with RNA extraction, reverse transcription, and PCR reagents · Confirm your iPS cells by differentiating between endogenous and exogenous gene expression
■ 产品应用
· Validating mouse embryonic stem cell status · Confirming iPS status
Code No.
Product
Size
631965
Pluripotency Check PCR Kit
Each
631966
Pluripotency Check PCR Primer Set
16 × 25 Rxns
■ 产品详情请点击:
Confirming the pluripotent status of an mES culture. Total RNA was extracted from ES-E14TG2a mouse embryonic stem cells (mES), followed by cDNA synthesis and amplification according to the protocol outlined in PT5086-2. M: 100 bp DNA Marker. Lane1, Total Oct3/4. Lane 2: Endogenous Oct3/4. Lane 3: Total Nanog. Lane 4: Endogenous Nanog. Lane 5: Total c-Myc. Lane 6: Endogenous c-Myc. Lane 7: Total Sox2. Lane 8: Endogenous Sox2. Lane 9: Total Klf4. Lane 10: Ecat1. Lane 11: ERas. Lane 12: Esg1. Lane 13: Rex1. Lane 14: GAPDH (control). Lane 15: Beta-Actin (control). Endogenous Klf4, data not shown.