Cell Meter BX590固定化细胞活性染料 货号22514-AAT Bioquest荧光染料

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Cell Meter BX590固定化细胞活性染料

Cell Meter BX590固定化细胞活性染料

Cell Meter BX590固定化细胞活性染料    货号22514 货号 22514 存储条件 在零下15度以下保存, 避免光照
规格 200 Tests 价格 2472
Ex (nm) 492 Em (nm) 579
分子量 溶剂 DMSO
产品详细介绍

简要概述

产品基本信息

货号:22514

产品名称:Cell Meter BX590固定化细胞活性染料

规格:200 Tests

储存条件:保存在冰箱-15℃干燥

保质期:12个月

 

产品物理化学光谱特性

分子量:N/A

激发波长(nm):492

发射波长(nm):579

 

适用仪器


流式细胞仪  
激发: 480nm激光
发射: 575/26nm滤波片
通道: PE通道
荧光显微镜  
激发: Cy3/TRITC滤波片组
发射: Cy3/TRITC滤波片组
推荐孔板: 黑色透明

 

 
产品介绍
 

从活细胞中区分和排除死细胞可以更清晰地分离和鉴定细胞群。Cell Meter BX590固定化细胞活性染料是一类细胞不可渗透的荧光活性染料,它们通过优化以匹配常见流式细胞仪的主要激发激光,例如350、405、488、633和647 nm。这些染料不渗入活细胞,但渗入膜受损的细胞。它们与含胺和硫醇的蛋白质以及其他细胞成分发生不可逆的反应。由于具有受损膜的死细胞或固定细胞更容易与Cell Meter BX590固定化细胞活性染料发生反应,因此比具有完整膜的活细胞的染色更亮,因此这些染料可用于评估哺乳动物细胞的活与死状态。使用这些染料时,需要考虑一些因素,例如,每种染料的滴定,以确保活细胞几乎没有染色。Cell Meter BX590固定化细胞活性染料通过优化,可在488 nm的蓝色激光激发并在590 nm发射。与其他商业上类似的活性染料相比,这种Cell Meter BX590固定化细胞活性染料更加耐用、稳定。金畔生物是AAT Bioquest的中国代理商,为您提供最优质的Cell Meter BX590固定化细胞活性染料。 

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图示

 

 

Cell Meter BX590固定化细胞活性染料    货号22514

图1.通过Cell Meter BX590固定化细胞活性染料检测Jurkat细胞活性。处理Jurkat细胞并用Cell Meter BX590(Cat#22514)染色,然后固定在3.7%甲醛中,并通过流式细胞仪进行分析。带有PE-TexasRed通道的死细胞数量(蓝峰)很容易与活细胞数量(红峰)区分开,在固定之前和之后获得的结果几乎相同。

 

 

参考文献

CFSE dilution to study human T and NK cell proliferation in vitro.
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Journal: Methods in enzymology (2020): 239-255

Using Carboxy Fluorescein Succinimidyl Ester (CFSE) to Identify Quiescent Glioblastoma Stem-Like Cells.
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Journal: Methods in molecular biology (Clifton, N.J.) (2018): 59-67

Estimates and impact of lymphocyte division parameters from CFSE data using mathematical modelling.
Authors: Mazzocco, Pauline and Bernard, Samuel and Pujo-Menjouet, Laurent
Journal: PloS one (2017): e0179768

Analysis of CFSE time-series data using division-, age- and label-structured population models.
Authors: Hross, Sabrina and Hasenauer, Jan
Journal: Bioinformatics (Oxford, England) (2016): 2321-9

Assessment of lymphocyte proliferation for diagnostic purpose: Comparison of CFSE staining, Ki-67 expression and 3H-thymidine incorporation.
Authors: Lašťovička, Jan and Rataj, Michal and Bartůňková, Jiřina
Journal: Human immunology (2016): 1215-1222

Poor association of allergen-specific antibody, T- and B-cell responses revealed with recombinant allergens and a CFSE dilution-based assay.
Authors: Eckl-Dorna, J and Campana, R and Valenta, R and Niederberger, V
Journal: Allergy (2015): 1222-9

Quality control of extracorporeal photochemotherapy: Proliferation assay using CFSE validated according to ISO 15189:2007 standards.
Authors: Faivre, Lionel and Lecouflet, Lucie and Liu, Wang-Qing and Khadher, Isabelle and Lahaie, Camille and Vidal, Michel and Legouvello, Sabine and Beaumont, Jean-Louis and Bierling, Philippe and Rouard, Hélène and Birebent, Brigitte
Journal: Cytometry. Part B, Clinical cytometry (2015): 30-9

Mathematical models for CFSE labelled lymphocyte dynamics: asymmetry and time-lag in division.
Authors: Luzyanina, Tatyana and Cupovic, Jovana and Ludewig, Burkhard and Bocharov, Gennady
Journal: Journal of mathematical biology (2014): 1547-83

Quality control of extracorporeal photochemotherapy: Proliferation assay using CFSE validated according to ISO 15189:2007 standards.
Authors: Lionel, Faivre and Lucie, Lecouflet and Wang-Qing, Liu and Isabelle, Khadher and Camille, Lahaie and Michel, Vidal and Sabine, Legouvello and Jean-Louis, Beaumont and Philippe, Bierling and Hélène, Rouard and Brigitte, Birebent
Journal: Cytometry. Part B, Clinical cytometry (2014)

Asymmetry of Cell Division in CFSE-Based Lymphocyte Proliferation Analysis.
Authors: Bocharov, Gennady and Luzyanina, Tatyana and Cupovic, Jovana and Ludewig, Burkhard
Journal: Frontiers in immunology (2013): 264

说明书
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