活性氧 Cell Meter 荧光法胞内总ROS检测试剂盒 适合于流式细胞仪 货号22904-AAT Bioquest荧光染料

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活性氧 Cell Meter 荧光法胞内总ROS检测试剂盒 适合于流式细胞仪

活性氧 Cell Meter 荧光法胞内总ROS检测试剂盒 适合于流式细胞仪

活性氧 Cell Meter 荧光法胞内总ROS检测试剂盒 适合于流式细胞仪      货号22904 货号 22904 存储条件 在零下15度以下保存, 避免光照
规格 100 Tests 价格 3924
Ex (nm) 490 Em (nm) 520
分子量 溶剂
产品详细介绍

简要概述

活性氧(ROS)是氧正常代谢的天然副产物,在细胞信号传导中起重要作用。但是,在与氧化应激相关的状态下,ROS水平会急剧增加。 ROS的积累会严重破坏细胞结构。氧化应激在心血管疾病,糖尿病,骨质疏松症,中风,炎性疾病,许多神经退行性疾病和癌症中的作用已得到公认。 ROS检测将有助于确定氧化应激如何调节各种细胞内途径。 Cell Meter 荧光法胞内总ROS检测试剂盒使用我们独特的Amplite ROS Green荧光定量活细胞中的ROS,Amplite ROS Green具有细胞渗透性。与ROS反应时会生成绿色荧光。 Cell Meter 荧光法胞内总ROS检测试剂盒提供了一种灵敏的一步荧光测定法,可在培养一小时后检测活细胞中的细胞内ROS。该试剂盒针对流式细胞仪应用进行了优化,其信号可以通过Ex / Em = 490/520 nm(FL1通道)进行检测。金畔生物是AAT Bioquest的中国代理商,为您提供最优质的Cell Meter 荧光法胞内总ROS检测试剂盒。 

活性氧(ROS)篇:包含总ROS和多种活性氧离子检测试剂大全

 

适用仪器


流式细胞仪  
激发: 488nm激光
发射: 530/30nm滤波片
通道: FITC通道

产品说明书

样品实验方案

简要概述

1.准备密度为0.5-1×106细胞/ mL的细胞
2.在0.5 mL细胞悬液中加入1 µL 500X Amplite ROS Green
3.在37℃下将细胞染色1小时
4.处理细胞以诱导ROS
5.使用带有FL1通道的流式细胞仪分析细胞(Ex / Em = 490/520 nm)

 

溶液配制

1.储备溶液配制

        除非另有说明,否则所有未使用的储备溶液应分成一次性等分试样,并在制备后储存在-20°C。 避免反复冻融循环。

Amplite ROS绿色储备溶液(500X):将100 µL DMSO(组分C)添加到Amplite ROS Green(组分A)的小瓶中,并充分混合以制成500X Amplite ROS Green储备液。 避光。 注意:要存放,请密封管。有关细胞样品制备的指南,请点击查看

 

操作步骤

1.对于每个样品,以0.5×105至1×106细胞/ mL的密度在0.5 mL测定缓冲液(组分B)或自备缓冲液中制备细胞。注意:应单独评估每个细胞系,以确定诱导ROS的最佳细胞密度。

2.将1 µL 500X Amplite ROS Green储备溶液加入0.5 mL细胞悬液中。

3.在37ºC下孵育1小时。注意:对于贴壁细胞,用0.5 mM EDTA轻轻提起细胞以保持细胞完整,并在与Amplite ROS Green孵育之前用含血清的培养基洗涤细胞一次。合适的孵育时间取决于所用的单个细胞类型和测试化合物,优化每个实验的孵育时间。

4.通过在所需的缓冲液(例如PBS或HBSS)中添加50 µL 11X测试化合物来处理细胞。对于对照孔(未处的细胞),添加相应量的缓冲液。

5.将细胞在37ºC下孵育,以诱导ROS(避光)。注意:我们在37℃下用100 µM TBHP(氢过氧化叔丁基)处理Jurkat细胞30分钟,以诱导ROS。有关详细信息,请参见图1。

6.使用具有FL1通道(Ex / Em = 490/520 nm)的流式细胞仪检测荧光强度。

 

图示

活性氧 Cell Meter 荧光法胞内总ROS检测试剂盒 适合于流式细胞仪      货号22904

图1.使用Cell Meter 荧光法细胞内总ROS活性测定试剂盒在TBHP处理后检测Jurkat细胞中的细胞内ROS。 将细胞与Amplite ROS Green在37°C孵育1小时。 然后将细胞在不使用(蓝色)或(红色)100 µM TBHP的情况下在37°C下处理30分钟。 使用流式细胞仪(BD FACSCalibur)在FL1通道处检测荧光信号。

 

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Notoginsenoside R1 attenuates high glucose-induced endothelial damage in rat retinal capillary endothelial cells by modulating the intracellular redox state
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Journal: Drug Design, Development and Therapy (2017): 3343

Good hydration and cell-biological performances of superparamagnetic calcium phosphate cement with concentration-dependent osteogenesis and angiogenesis induced by ferric iron
Authors: Zhang, J and Shi, HS and Liu, JQ and Yu, T and Shen, ZH and Ye, JD
Journal: Journal of Materials Chemistry B (2015): 8782–8795

Topiramate Protects Pericytes from Glucotoxicity: Role for Mitochondrial CA VA in Cerebromicrovascular Disease in Diabetes
Authors: Patrick, Ping and Price, Tulin O and Diogo, Ana L and Sheibani, Nader and Banks, William A and Shah, Gul N
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Down-regulated peroxisome proliferator-activated receptor γ (PPARγ) in lung epithelial cells promotes a PPARγ agonist-reversible proinflammatory phenotype in chronic obstructive pulmonary disease (COPD)
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Xanthine oxidase inhibition by febuxostat attenuates experimental atherosclerosis in mice
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说明书
活性氧 Cell Meter 荧光法胞内总ROS检测试剂盒 适合于流式细胞仪 .pdf