iFluor 647-PEG12-dUTP *1 mM Tris 缓冲液 (pH 7.5)* 货号17043-AAT Bioquest荧光染料

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iFluor 647-PEG12-dUTP *1 mM Tris 缓冲液 (pH 7.5)*

iFluor 647-PEG12-dUTP *1 mM Tris 缓冲液 (pH 7.5)*

iFluor 647-PEG12-dUTP *1 mM Tris 缓冲液 (pH 7.5)*    货号17043 货号 17043 存储条件 在零下15度以下保存, 避免光照
规格 25 nmoles 价格 1164
Ex (nm) 656 Em (nm) 670
分子量 2293.72 溶剂 Water
产品详细介绍

简要概述

产品基本信息

货号:17043

产品名称:iFluor 647-PEG12-dUTP *1 mM Tris 缓冲液 (pH 7.5)*

规格:25 nmoles

储存条件:-15℃避光防潮

保质期:12个月

 

产品物理化学光谱特性

分子量:2293.72

溶剂:水

外观:液体

激发波长(nm):656

发射波长(nm):670

 

产品介绍

染料修饰的脱氧尿苷 5′-三磷酸是通过常规酶促掺入方法(如逆转录、切口平移、随机引物标记或 PCR)生产染料标记 DNA 的最常用方法之一。 这种酶促荧光标记方法广泛用于 FISH 探针和基于微阵列的实验。 这种 iFluor 647-PEG12-dUTP 缀合物具有与 ChromaTide Alexa Fluor 647-dUTP 相同的光谱特性。 其 PEG12 间隔区使其更有效地通过聚合酶整合到 DNA 中。 它用作带有 SpectrumFRed 滤光片组的远红色荧光颜色。 它提供了具有高光稳定性且不受 pH 影响的明亮信号。

点击查看光谱

 

参考文献

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dUTPs conjugated with zwitterionic Cy3 or Cy5 fluorophore analogues are effective substrates for DNA amplification and labelling by Taq polymerase.
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Journal: Nucleic acids research (2018): e73

Mass-spectrometry analysis of modifications at DNA termini induced by DNA polymerases.
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Separation of replication and transcription domains in nucleoli.
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Journal: Journal of structural biology (2014): 259-66

A prenatally ascertained, maternally inherited 14.8 Mb duplication of chromosomal bands Xq13.2-q21.31 associated with multiple congenital abnormalities in a male fetus.
Authors: Sismani, C and Donoghue, J and Alexandrou, A and Karkaletsi, M and Christopoulou, S and Konstantinidou, A E and Livanos, P and Patsalis, P C and Velissariou, V
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[Establishment of a hydrogel chip for high-throughput detection of Y chromosome microdeletions].
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Journal: Zhonghua nan ke xue = National journal of andrology (2012): 109-14

Selection of reliable reference genes for gene expression study in nasopharyngeal carcinoma.
Authors: Guo, Yi and Chen, Jia-xin and Yang, Shu and Fu, Xu-ping and Zhang, Zheng and Chen, Ke-he and Huang, Yan and Li, Yao and Xie, Yi and Mao, Yu-min
Journal: Acta pharmacologica Sinica (2010): 1487-94

Time-resolved three-dimensional molecular tracking in live cells.
Authors: Wells, Nathan P and Lessard, Guillaume A and Goodwin, Peter M and Phipps, Mary E and Cutler, Patrick J and Lidke, Diane S and Wilson, Bridget S and Werner, James H
Journal: Nano letters (2010): 4732-7

[Expression of COX10 in human non-obstructive azoospermia testes].
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Journal: Zhonghua nan ke xue = National journal of andrology (2009): 599-603

[Expression of cell cycle molecules in human azoospermic testes].
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说明书
iFluor 647-PEG12-dUTP *1 mM Tris 缓冲液 (pH 7.5)*.pdf