Helixyte mRNA转染试剂 货号60031-AAT Bioquest荧光染料

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Helixyte mRNA转染试剂

Helixyte mRNA转染试剂

Helixyte mRNA转染试剂    货号60031 货号 60031 存储条件 在2-8度冷藏保存, 避免光照
规格 5 ml 价格 11628
Ex (nm) Em (nm)
分子量 溶剂 Water


Helixyte mRNA 转染试剂是一种功能强大且用途广泛的转染试剂,它是将更多的 mRNA 引入真核细胞,或更具体地说,引入动物细胞。 它在各种贴壁和悬浮细胞系中提供高转染效率,包括难以转染的细胞。不需要核摄取,这导致比 DNA 转染更快的蛋白质表达,而没有基因组整合的风险。 Helixyte mRNA转染试剂的低毒性可提高转染细胞的生存能力。 Helixyte mRNA 转染试剂不需要特殊培养基,与大多数商业转染试剂相比更易于使用。




2.制备 Helixyte mRNA 转染试剂-RNA 混合物
3.将 Helixyte mRNA 转染试剂-RNA 混合物添加到细胞培养物中



1.在转染时将细胞培养至 ~ 90% 汇合。
2.转染前更换新鲜培养基。例如,6 孔板每孔更换 2 mL 培养基,10 cm 板更换 6 mL 培养基。



1.Helixyte mRNA 转染试剂-RNA 混合物
2.将 2.5 µg mRNA 与 200 µL 无血清培养基混合。
3.在步骤 1 中加入 7.5 µL Helixyte mRNA 转染试剂。
4.混匀并在室温下孵育 20 分钟。
注意:Helixyte mRNA 转染试剂与 mRNA 的比例需要针对不同的细胞系进行优化。 通常,Helixyte mRNA 转染试剂 (µL) 与 mRNA (µg) 的比率 =(3 至 5 µL)至 1 µg。


组分 6孔板(每孔)
新鲜培养基 2ml
纯化的mRNA ~2.5ug
无血清培养基 200ul
Helixyte mRNA 转染试剂 -7.5ul




1.1将 Helixyte mRNA 转染试剂 – mRNA 混合物加入培养板并培养过夜。
注意:重组蛋白表达可以在转染后的8小时内检测到。 转染后约24小时可观察到最大表达水平。

Helixyte mRNA转染试剂    货号60031

图 1. HeLa 细胞中的转染效率比较(上图)和细胞毒性比较(下图)。 HeLa 细胞在 6 孔板中培养至~90% 汇合。 2.5 µg mRNA 分别用 Lipofactamin MessengerMAX 和 Helixyte mRNA 转染试剂转染。 转染后 18 小时,使用带有 FITC 通道的荧光显微镜(上图)拍摄图像。 Lipofactamin MessengerMAX 和 Helixyte mRNA 转染试剂的转染效率相似。用 Helixyte mRNA 转染试剂转染的细胞看起来比用 Lipofatamin MessengerMAX 转染的细胞健康得多(下图)。



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Helixyte mRNA转染试剂.pdf