Cell Meter IX650固定化细胞活性染料 货号22529-AAT Bioquest荧光染料

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Cell Meter IX650固定化细胞活性染料

Cell Meter IX650固定化细胞活性染料

Cell Meter IX650固定化细胞活性染料    货号22529 货号 22529 存储条件 在零下15度以下保存, 避免光照
规格 200 Tests 价格 2472
Ex (nm) 811 Em (nm) 822
分子量 溶剂 DMSO
产品详细介绍

简要概述

产品基本信息

货号:22529

产品名称:Cell Meter IX650固定化细胞活性染料

规格:200 Tests

储存条件:保存在冰箱-15℃干燥

保质期:12个月

 

产品物理化学光谱特性

分子量:N/A

激发波长(nm):811

发射波长(nm):822

 

适用仪器


流式细胞仪  
激发: 634nm激光 or IR激光
发射: 780/50 nm or IR滤波片
滤波片: APC/Cy7 or IR滤波片组

 

产品介绍

从活细胞中区分和排除死细胞可以更清晰地分离和鉴定细胞群。Cell Meter IX650固定化细胞活性染料是一类细胞不可渗透的荧光活性染料,它们经过优化以匹配常见流式细胞仪的主要激发激光,例如350、405、488、633和647 nm。这些染料不渗入活细胞,但渗入膜受损的细胞。它们与含胺和硫醇的蛋白质以及其他细胞成分发生不可逆的反应。由于具有受损膜的死细胞或固定细胞更容易与Cell Meter IX650固定化细胞活性染料发生反应,因此比具有完整膜的活细胞的染色更亮,因此这些染料可用于评估哺乳动物细胞的活与死状态。使用这些染料时,需要考虑一些因素,例如,每种染料的滴定,以确保活细胞几乎没有染色。Cell Meter IX650固定化细胞活性染料经过优化,可以在808 nm的红色激光激发下以830 nm的波长发射。与其他商业上类似的活性染料相比,这种固定化细胞活性染料更加耐用、稳定。金畔生物是AAT Bioquest的中国代理商,为您提供最优质的Cell Meter IX650固定化细胞活性染料。 

点击查看光谱

 

图示

 

 

Cell Meter IX650固定化细胞活性染料    货号22529

图1.通过Cell Meter 固定化细胞活性染料检测Jurkat细胞活力。 处理Jurkat细胞并用Cell Meter IX830(Cat#22529)染色,然后固定在3.7%甲醛中,并通过流式细胞仪进行分析。 通过APC-Cy7通道可以很容易地将死细胞群体(蓝峰)与活细胞群体(红峰)区分开,在固定之前和之后可获得几乎相同的结果。

 

 

参考文献

Hsa_circ_0011385 accelerates the progression of thyroid cancer by targeting miR-361-3p.
Authors: Xia, Fada and Chen, Yong and Jiang, Bo and Bai, Ning and Li, Xinying
Journal: Cancer cell international (2020): 49

Immunomodulatory effects of some Namibian plants traditionally used for treating inflammatory diseases.
Authors: Du Preez, C I and Gründemann, C and Mumbengegwi, D R and Huber, R
Journal: Journal of ethnopharmacology (2020): 112683

UHPLC-Q/Orbitrap/MS/MS fingerprinting and antitumoral effects of Prosopis strombulifera (LAM.) BENTH. queous extract on allograft colorectal and melanoma cancer models.
Authors: Persia, Fabio Andrés and Troncoso, Mariana Elizabeth and Rinaldini, Estefanía and Simirgiotis, Mario and Tapia, Alejandro and Bórquez, Jorge and Mackern-Oberti, Juan Pablo and Hapon, María Belén and Gamarra-Luques, Carlos
Journal: Heliyon (2020): e03353

Evaluation of the Compatibility of Electric Tumor Treating Fields with Key Anti-tumoral T-Cell Functions.
Authors: Simchony, Hadar and Diamant, Diamant and Ram, Zvi and Volovitz, Ilan
Journal: The Israel Medical Association journal : IMAJ (2019): 503

Influence of traditionally used Nepalese plants on wound healing and immunological properties using primary human cells in vitro.
Authors: Zimmermann-Klemd, Amy M and Konradi, Viktoria and Steinborn, Carmen and Ücker, Annekathrin and Falanga, Chiara Madlen and Woelfle, Ute and Huber, Roman and Jürgenliemk, Guido and Rajbhandari, Meena and Gründemann, Carsten
Journal: Journal of ethnopharmacology (2019): 415-423

Lignins isolated from Prickly pear cladodes of the species Opuntia fícus-indica (Linnaeus) Miller and Opuntia cochenillifera (Linnaeus) Miller induces mice splenocytes activation, proliferation and cytokines production.
Authors: da Cruz Filho, Iranildo José and da Silva Barros, Bárbara Rafaela and de Souza Aguiar, Lethícia Maria and Navarro, Claudia Daniele Carvalho and Ruas, Juliana Silveira and de Lorena, Virgínia Maria Barros and de Moraes Rocha, George Jackson and Vercesi, Aníbal Eugênio and de Melo, Cristiane Moutinho Lagos and Maior, Ana Maria Souto
Journal: International journal of biological macromolecules (2019): 1331-1339

Nerigoside suppresses colorectal cancer cell growth and metastatic potential through inhibition of ERK/GSK3β/β-catenin signaling pathway.
Authors: Wen, Shi-Yuan and Chen, Yan-Yan and Deng, Chun-Miao and Zhang, Cui-Qiong and Jiang, Miao-Miao
Journal: Phytomedicine: international journal of phytotherapy and phytopharmacology (2019): 352-363

Parthenolide inhibits the proliferation and induces the apoptosis of human uveal melanoma cells.
Authors: Che, Song-Tian and Bie, Li and Li, Xu and Qi, Hui and Yu, Peng and Zuo, Ling
Journal: International journal of ophthalmology (2019): 1531-1538

Research on the effect and mechanism of antimicrobial peptides HPRP-A1/A2 work against Toxoplasma gondii infection.
Authors: Liu, Ran and Ni, Yangyue and Song, Jingwei and Xu, Zhipeng and Qiu, Jingfan and Wang, Lijuan and Zhu, Yuxiao and Huang, Yibing and Ji, Minjun and Chen, Yuxin
Journal: Parasite immunology (2019): e12619

Tracking keratinocytes and melanocytes using carboxyfluorescein hydroxysuccinimidyl ester staining.
Authors: Lönnqvist, Susanna and Junker, Johan P E and Sedell, Maria and Nyman, Erika and Kratz, Gunnar
Journal: PloS one (2019): e0221878

说明书
Cell Meter IX650固定化细胞活性染料.pdf