ddGTP [2′,3′-Dideoxyguanosine-5′-triphosphate]

	货号	17210	存储条件	在零下15度以下保存, 避免光照
	规格	1 umole	价格	2472
	Ex (nm)		Em (nm)
	分子量	491.18	溶剂	Water
	产品详细介绍

简要概述

产品基本信息

货号：17210

产品名称：ddGTP [2′,3′-Dideoxyguanosine-5′-triphosphate]

规格：1 umole

储存条件：-15℃避光防潮

保质期：24个月

产品物理化学光谱特性

分子量：491.18

溶剂：水

产品介绍

Sanger测序，也称为链终止法，是一种基于DNA聚合酶选择性掺入链终止双脱氧核苷酸（ddNTPs）的DNA测序技术。它是由Frederick Sanger及其同事于1977年开发的。尽管新的NGS技术由于其较高的通量能力和较低的每份样品成本而在临床研究实验室中变得很普遍，但Sanger测序仍具有99.99％的准确度，仍然是临床的“黄金标准”研究排序。 dd-GTP是执行Sanger测序的四个关键ddNTP组件之一。

参考文献

Detection and genotyping of human papillomavirus DNA in cervical cancer tissues with fluorescence polarization.
Authors: Gao, Yan-E and Zhang, Ju and Wu, Jing and Chen, Zhong-Can and Yan, Xiao-Jun
Journal: Sheng wu hua xue yu sheng wu wu li xue bao Acta biochimica et biophysica Sinica (2003): 1029-34

Microcapillary reactors using solid-phase DNA sequencing for direct sample introduction into slab gels.
Authors: Xu, Y and Bruch, R C and Soper, S A
Journal: BioTechniques (2000): 904-8, 910, 912

Isolation and characterization of a dideoxyguanosine triphosphate-resistant mutant of human immunodeficiency virus reverse transcriptase.
Authors: Prasad, V R and Lowy, I and de los Santos, T and Chiang, L and Goff, S P
Journal: Proceedings of the National Academy of Sciences of the United States of America (1991): 11363-7

Herpes simplex virus type 1 and human DNA polymerase interactions with 2′-deoxyguanosine 5′-triphosphate analogues. Kinetics of incorporation into DNA and induction of inhibition.
Authors: Reardon, J E
Journal: The Journal of biological chemistry (1989): 19039-44