7-Deaza-7-Propargylamino-3′-azidomethyl-dGTP 货号17092-AAT Bioquest荧光染料

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品,欢迎访问AAT Bioquest荧光染料官网了解更多信息。

7-Deaza-7-Propargylamino-3′-azidomethyl-dGTP

7-Deaza-7-Propargylamino-3′-azidomethyl-dGTP

7-Deaza-7-Propargylamino-3'-azidomethyl-dGTP    货号17092 货号 17092 存储条件 在零下15度以下保存, 避免光照
规格 50 nmoles 价格 4368
Ex (nm) Em (nm)
分子量 614.30 溶剂 Water
产品详细介绍

简要概述

产品基本信息

货号:17092

产品名称:7-Deaza-7-Propargylamino-3′-azidomethyl-dGTP

规格:50 nmoles

储存条件:-15℃避光防潮

保质期:24个月

 

产品物理化学光谱特性

分子量:614.30

溶剂:水

 

产品介绍

7-Deaza-7-Propargylamino-3′-azidomethylmethyl-dGTP是制备用于下一代测序(NGS)的荧光缀合物的关键组成部分。 NGS使用与早期Sanger测序相似的链终止方法,但NGS是通过荧光标记的核苷酸类似物作为扩增反应的可逆终止剂来进行的。 NGS依赖于可逆的DNA聚合阻断,而Sanger测序则使用ddNTP不可逆转的DNA聚合阻断。 NGS的另一个不同特征是通过桥式PCR进行体外克隆扩增以增加待测序分子的数量。在此平台上,片段与固定在固体表面上的引物连接,进行原位扩增,生成具有相同分子的DNA簇。在每个循环中,同时添加可逆终止的四个核苷酸,并通过它们互补的聚合酶掺入。通过将3′-OH基团替换为3′-o-叠氮基甲基,这些核苷酸被化学封闭,以防止聚合酶在每个循环中掺入一个以上的核苷酸。掺入核苷酸后,在不同通道中针对不同碱基测量荧光信号。关于下一循环,洗涤未掺入的核苷酸,并用TCEP去除3’端的化学封锁。一旦收集到荧光信号,就会开始一个新的循环,重复此动态过程,直到完成每个片段的测序为止。总之,NGS测序反应分三个步骤进行:核苷酸的添加,成像和通过荧光团裂解的3′-OH再生。

 

参考文献

A novel target enrichment strategy in next-generation sequencing through 7-deaza-dGTP-resistant enzymatic digestion.
Authors: Peng, Peng and Xu, Yanjuan and Di Bisceglie, Adrian M and Fan, Xiaofeng
Journal: BMC research notes (2020): 445

SAMHD1-deficient fibroblasts from Aicardi-Goutières Syndrome patients can escape senescence and accumulate mutations.
Authors: Franzolin, Elisa and Coletta, Sara and Ferraro, Paola and Pontarin, Giovanna and D’Aronco, Giulia and Stevanoni, Martina and Palumbo, Elisa and Cagnin, Stefano and Bertoldi, Loris and Feltrin, Erika and Valle, Giorgio and Russo, Antonella and Bianchi, Vera and Rampazzo, Chiara
Journal: FASEB journal : official publication of the Federation of American Societies for Experimental Biology (2020): 631-647

Suppressors of dGTP Starvation in Escherichia coli.
Authors: Itsko, Mark and Schaaper, Roel M
Journal: Journal of bacteriology (2017)

Single-tube, highly parallel mutation enrichment in cancer gene panels by use of temperature-tolerant COLD-PCR.
Authors: Castellanos-Rizaldos, Elena and Richardson, Katherine and Lin, Rui and Wu, Grant and Makrigiorgos, Mike G
Journal: Clinical chemistry (2015): 267-77

Transcriptome analysis of Capsicum annuum varieties Mandarin and Blackcluster: assembly, annotation and molecular marker discovery.
Authors: Ahn, Yul-Kyun and Tripathi, Swati and Kim, Jeong-Ho and Cho, Young-Il and Lee, Hye-Eun and Kim, Do-Sun and Woo, Jong-Gyu and Cho, Myeong-Cheoul
Journal: Gene (2014): 494-9

Deep sequencing analysis of mutations resulting from the incorporation of dNTP analogs.
Authors: Petrie, Katherine L and Joyce, Gerald F
Journal: Nucleic acids research (2010): 8095-104

Design and synthesis of a photocleavable fluorescent nucleotide 3′-O-allyl-dGTP-PC-Bodipy-FL-510 as a reversible terminator for DNA sequencing by synthesis.
Authors: Meng, Qinglin and Kim, Dae Hyun and Bai, Xiaopeng and Bi, Lanrong and Turro, Nicholas J and Ju, Jingyue
Journal: The Journal of organic chemistry (2006): 3248-52

说明书
7-Deaza-7-Propargylamino-3′-azidomethyl-dGTP.pdf