Screen Quest 活细胞cAMP检测 货号36382-AAT Bioquest荧光染料

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Screen Quest 活细胞cAMP检测

Screen Quest 活细胞cAMP检测

Screen Quest 活细胞cAMP检测    货号36382 货号 36382 存储条件 在零下15度以下保存, 避免光照
规格 100 Tests 价格 17748
Ex (nm) 490 Em (nm) 520
分子量 溶剂
产品详细介绍

简要概述

产品基本信息

货号:36382

产品名称:Screen Quest 活细胞cAMP检测

规格:100 Tests

 

产品介绍

G蛋白偶联受体(GPCR)是药物发现计划中最大的靶向受体之一。钙通量(通过Gq途径耦合)分析是筛选GPCR靶点的首选方法。然而,超过60%的已知GPCRs信号通过腺苷酸环化酶活性偶联到cAMP。现有的cAMP检测方法不仅需要细胞裂解,而且缺乏时间和空间分辨率。Screen Quest 活细胞cAMP检测以高通量的形式提供细胞内cAMP变化的实时监测,无需细胞裂解步骤。该方法通过含有外源性环核苷酸门控通道(CNGC)或混杂G蛋白Gα16的细胞系进行。该通道被细胞内cAMP水平升高激活,导致离子通量和细胞膜去极化,可通过荧光钙(如Calbryte 520 AM、Cal-520 AM、Fluo-8 AM或Fluo-4 AM和相应的免洗钙试剂盒)或荧光膜电位染料检测到。Gα16与特异性非Gq偶联受体的共表达将在受体刺激下产生细胞内钙信号。Screen Quest 活细胞cAMP检测提供细胞系和试剂,用于使用FLIPR、FDSS或其他等效的荧光酶标仪检测细胞内cAMP的变化。它已成功地用于测定Gs和Gi偶联GPCR活性。金畔生物是AAT Bioquest的中国代理商,为您提供最优质的Screen Quest 活细胞cAMP检测。 

 

图示

Screen Quest 活细胞cAMP检测    货号36382

图1. Screen Quest 活细胞cAMP测定原理

 

参考文献

A cardiac mitochondrial cAMP signaling pathway regulates calcium accumulation, permeability transition and cell death
Authors: Wang Z, Liu D, Varin A, Nicolas V, Courilleau D, Mateo P, Caubere C, Rouet P, Gomez AM, V and ecasteele G, Fischmeister R, Brenner C.
Journal: Cell Death Dis (2016): e2198

Activation of P2X7 and P2Y11 purinergic receptors inhibits migration and normalizes tumor-derived endothelial cells via cAMP signaling
Authors: Avanzato, D and Genova, T and Pla, A Fiorio and Bernardini, M and Bianco, S and Bussolati, B and Mancardi, D and Giraudo, E and Maione, F and Cassoni, P and others
Journal: Scientific Reports (2016)

Changes in the Arabidopsis thaliana Proteome Implicate cAMP in Biotic and Abiotic Stress Responses and Changes in Energy Metabolism
Authors: Alqurashi M, Gehring C, Marondedze C.
Journal: Int J Mol Sci (2016): 852

Odor-induced cAMP production in Drosophila melanogaster olfactory sensory neurons
Authors: Miazzi F, Hansson BS, Wicher D.
Journal: J Exp Biol (2016): 1798

Role of the cAMP Pathway in Glucose and Lipid Metabolism
Authors: Ravnskjaer K, Madiraju A, Montminy M.
Journal: Handb Exp Pharmacol (2016): 29

The pleiotropic role of exchange protein directly activated by cAMP 1 (EPAC1) in cancer: implications for therapeutic intervention
Authors: Almahariq M, Mei FC, Cheng X.
Journal: Acta Biochim Biophys Sin (Shanghai) (2016): 75

cAMP-Induced Histones H3 Dephosphorylation Is Independent of PKA and MAP Kinase Activations and Correlates With mTOR Inactivation
Authors: Rodriguez P, Rojas J.
Journal: J Cell Biochem (2016): 741

A cAMP Biosensor-Based High-Throughput Screening Assay for Identification of Gs-Coupled GPCR Ligands and Phosphodiesterase Inhibitors
Authors: Vedel L, Brauner-Osborne H, Mathiesen JM.
Journal: J Biomol Screen (2015): 849

Cardiac Hypertrophy Is Inhibited by a Local Pool of cAMP Regulated by Phosphodiesterase 2
Authors: Zoccarato A, Surdo NC, Aronsen JM, Fields LA, Mancuso L, Dodoni G, Stangherlin A, Livie C, Jiang H, Sin YY, Gesellchen F, Terrin A, Baillie GS, Nicklin SA, Graham D, Szabo-Fresnais N, Krall J, V and eput F, Movsesian M, Furlan L, Corsetti V, Hamilton G, Lefkimmiatis K, Sjaastad I, Zaccolo M.
Journal: Circ Res (2015): 707

Cardiac cAMP: production, hydrolysis, modulation and detection
Authors: Boularan C, Gales C.
Journal: Front Pharmacol (2015): 203

说明书
Screen Quest 活细胞cAMP检测.pdf