Cellartis Y50035 Cellartis® Intestinal Epithelial Cells (from ChiPSC18) Kit 1 Kit酶试剂盒

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Human Stem Cell Derived Intestinal Epithelial Cells
品牌 Code No. 产品名称 包装量 价格(元) 说明书 数量
Cellartis Y50035 Cellartis® Intestinal Epithelial Cells (from ChiPSC18) Kit 1 Kit ¥20,754 Cellartis                      Y50035           Cellartis® Intestinal Epithelial Cells (from ChiPSC18) Kit            1 Kit Cellartis                      Y50035           Cellartis® Intestinal Epithelial Cells (from ChiPSC18) Kit            1 Kit Cellartis                      Y50035           Cellartis® Intestinal Epithelial Cells (from ChiPSC18) Kit            1 Kit
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来源于人类iPS细胞的小肠上皮细胞
(Intestinal Epithelial Cells Derived from Human Induced Pluripotent Stem Cells)
Cellartis Intestinal Epithelial Cells (from ChiPSC18) 是来源于人iPS细胞的小肠上皮细胞,具备肠上皮样特征,包括形成紧密连接、表达绒毛蛋白villin和肠转录因子CDX2。与人结肠癌Caco-2细胞系相比,该细胞具有更高的代谢酶CYP3A4和转运蛋白PEPT1的表达,为药物代谢吸收、药物相互作用评估提供更好的预测结果。
Cellartis Intestinal Epithelial Cells (from ChiPSC18) Kit 包括1管冻存的细胞Cellartis Intestinal Epithelial Cells (from ChiPSC18)和1个试剂盒Cellartis IEC Maturation Kit。解冻的Cellartis Intestinal Epithelial Cells (from ChiPSC18)使用Cellartis IEC Maturation Kit预先培养5天后,可用于各种检测。
 
■ 产品特点
· 解冻5天内细胞形成紧密连接,解冻5-8天后可用于检测
· 细胞高表达肠上皮标志物 (villin、CDX2等)
· 与Caco-2细胞相比,拥有更高的代谢酶CYP3A4和转运蛋白PEPT1的表达
· 细胞形成一个功能性的、可渗透的屏障,可用于预测药物在体内的渗透性和吸收
 
■ 产品应用
· 药物在小肠的吸收和代谢评价
· 药物研发中的ADME分析
· 药物渗透性检测
 
■ 产品组成
· 1 vial of~4.8 x 106 cells
· 1 Cellartis IEC Maturation Kit(IEC Maturation Basal Medium;IEC Supplement A;IEC Supplement B;IEC Supplement C;IEC Supplement D)
 
Cellartis                      Y50035           Cellartis® Intestinal Epithelial Cells (from ChiPSC18) Kit            1 Kit
 
实验例
Cellartis                      Y50035           Cellartis® Intestinal Epithelial Cells (from ChiPSC18) Kit            1 Kit
图1. 紧密连接蛋白ZO-1的免疫荧光染色结果显示,解冻后第5天,hiPSC-derived intestinal epithelial cells (IEC)细胞间形成紧密连接(如闭锁小带zonula occludens)。
 
Cellartis                      Y50035           Cellartis® Intestinal Epithelial Cells (from ChiPSC18) Kit            1 Kit
图2. 在解冻后第7天,与Caco-2细胞相比,hiPSC-derived intestinal epithelial cells (IEC)中高表达肠上皮标志物villin和CDX2。
 
Cellartis                      Y50035           Cellartis® Intestinal Epithelial Cells (from ChiPSC18) Kit            1 Kit
图3. 在解冻后第7天,与Caco-2细胞相比,hiPSC-derived intestinal epithelial cells (IEC)中高表达药物代谢酶CYP3A4和药物转运蛋白PEPT1。
 
 
■ 产品详情请点击:Cellartis                      Y50035           Cellartis® Intestinal Epithelial Cells (from ChiPSC18) Kit            1 Kit