Cell Meter 荧光法细胞电位检测试剂盒 货号35000-AAT Bioquest荧光染料

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Cell Meter 荧光法细胞电位检测试剂盒

Cell Meter 荧光法细胞电位检测试剂盒

Cell Meter 荧光法细胞电位检测试剂盒    货号35000 货号 35000 存储条件 Multiple
规格 100 Tests 价格 8772
Ex (nm) Em (nm)
分子量 溶剂
产品详细介绍

简要概述

产品基本信息

货号:35000

产品名称:Cell Meter 荧光法细胞电位检测试剂盒

规格:100 Tests

保质期:12个月

 

适用仪器


荧光酶标仪  
激发: 405nm
发射: 460和580nm
cutoff: 435和515nm
推荐孔板: 黑色透明
读取模式: 底读模式
其他仪器
FDSS, FLIPR, ViewLux, ArrayScan, FlexStation, IN Cell Analyzer

 

产品介绍

几乎所有质膜在其上都具有电势,内部通常相对于外部为负。信号是通过打开或关闭膜上某一点的离子通道而产生的,从而在膜电位上产生局部变化。电场的这种变化会很快受到膜中相邻或较远的离子通道的影响。然后,由于电势变化,这些离子通道可以打开或关闭,从而产生信号。 Cell Meter 荧光细胞电位检测试剂盒使用FRET对(VSB 405和VSR 555)来检测细胞膜电位的变化。亲脂性VSB 405主要位于脂质膜的外层,而VSR 555的定位对细胞膜电位敏感。在静止状态下,的内层具有相对负的电势,从而使VSR 555主要位于细胞膜的外层附近,因此靠近蓝色荧光VSB405,从而导致从蓝色(VSB 405)到蓝色荧光的有效转移。红色(VSR 555)。当细胞去极化时,VSR 555易位到细胞膜的内层,从而分离FRET对并破坏FRET。蓝色/红色荧光比与细胞电位成正比。该检测法可用于筛选调节离子通道的化合物。

 

图示

Cell Meter 荧光法细胞电位检测试剂盒    货号35000

图1.使用Cell Meter 荧光细胞电位检测试剂盒检测HeLa细胞中的膜电位。根据试剂盒说明对HeLa细胞进行染色,并用去极化溶液(164.5 mM KCl,2 mM CaCl2、1 mM MgCl2、10 mM葡萄糖,20 mM HEPES,pH 7.4)刺激。使用FlexStation 3(分子设备)记录响应。

 

参考文献

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A cell-sparing electric field stimulation technique for high-throughput screening of voltage-gated ion channels.
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