1mM Biotin-11-dUTP溶液 CAS 86303-25-5 货号17016-AAT Bioquest荧光染料

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品,欢迎访问AAT Bioquest荧光染料官网了解更多信息。

1mM Biotin-11-dUTP溶液 CAS 86303-25-5

1mM Biotin-11-dUTP溶液 CAS 86303-25-5

1mM Biotin-11-dUTP溶液  CAS 86303-25-5    货号17016 货号 17016 存储条件 在零下15度以下保存, 避免光照
规格 25 nmoles 价格 1272
Ex (nm) Em (nm)
分子量 928.62 溶剂 Water
产品详细介绍

简要概述

产品基本信息

货号:17016

产品名称:1mM 生物素-11-dUTP溶液

CAS:86303-25-5

规格:25nmolse

储存条件:保存在冰箱-15℃干燥

保质期:12个月

 

产品物理化学光谱特性

分子量:928.62

外观:液体

溶剂:水

激发波长(nm):N/A

发射波长(nm):N/A

 

产品介绍

生物素修饰的脱氧尿苷5′-三磷酸广泛用于各种非放射性DNA标记反应,包括切口平移,随机引物标记,cDNA标记和3’末端标记。已显示生物素化的探针以与非生物素化探针相同的速率和程度与同源核酸杂交。杂交的生物素化DNA探针可以通过抗生物素蛋白和链霉抗生物素蛋白检测。生物素-11-dUTP可以通过切口平移,随机引发,3′-末端标记或在PCR过程中酶促地掺入DNA中。数字’11’是dUTP和生物素之间的接头主链中的碳原子数。接头越长,生物素与抗生物素蛋白的相互作用就越有效。另一方面,接头越短,dUTP更有效地掺入DNA。建议连接子’11’的长度对于大多数应用是最佳的。 Bio-11-dUTP用于在各种杂交应用中生产生物素化的DNA探针,包括Southern印迹,Northern印迹,斑点印迹,固定细胞和组织。

 

参考文献

A visual DNA chip for simultaneous detection of hepatitis B virus, hepatitis C virus and human immunodeficiency virus type-1
Authors: Wen JK, Zhang XE, Cheng Z, Liu H, Zhou YF, Zhang ZP, Yang JH, Deng JY.
Journal: Biosens Bioelectron (2004): 685

Fluorescent DNA hybridization probe preparation using amine modification and reactive dye coupling
Authors: Cox WG, Singer VL.
Journal: Biotechniques (2004): 114

Aminomodified nucleobases: functionalized nucleoside triphosphates applicable for SELEX
Authors: Schoetzau T, Langner J, Moyroud E, Roehl I, Vonhoff S, Klussmann S.
Journal: Bioconjug Chem (2003): 919

Simple method for preparation of fluor/hapten-labeled dUTP
Authors: Nimmakayalu M, Henegariu O, Ward DC, Bray-Ward P.
Journal: Biotechniques (2000): 518

Topology of yeast RNA polymerase II subunits in transcription elongation complexes studied by photoaffinity cross-linking
Authors: Wooddell CI, Burgess RR.
Journal: Biochemistry (2000): 13405

Quantitative analysis of polymerase chain reaction products using biotinylated dUTP incorporation
Authors: Duplaa C, Couffinhal T, Labat L, Moreau C, Lamaziere JM, Bonnet J.
Journal: Anal Biochem (1993): 229

A non-radioisotopic reverse transcriptase assay using biotin-11-deoxyuridinetriphosphate on primer-immobilized microtiter plates
Authors: Urabe T, Sano K, Tanno M, Mizoguchi J, Otani M, Lee MH, Takasaki T, Kusakabe H, Imagawa DT, Nakai M.
Journal: J Virol Methods (1992): 145

Affinity isolation of active murine erythroleukemia cell chromatin: uniform distribution of ubiquitinated histone H2A between active and inactive fractions
Authors: Dawson BA, Herman T, Haas AL, Lough J.
Journal: J Cell Biochem (1991): 166

Non-radioactive labeling and detection of nucleic acids. IV. Synthesis and properties of digoxigenin-modified 2′-deoxyuridine-5′-triphosphates and a photoactivatable analog of digoxigenin (photodigoxigenin)
Authors: Muhlegger K, Huber E, von der Eltz H, Ruger R, Kessler C.
Journal: Biol Chem Hoppe Seyler (1990): 953

 

相关产品

产品名称 货号
生物素-X氨三乙酸 CAS 856661-92-2 Cat#3009
生物素-4-荧光素 CAS 1032732-74-3 Cat#3006
生物素 CAS 58-85-5 Cat#3001

说明书
1mM Biotin-11-dUTP溶液 CAS 86303-25-5.pdf