Clontech 632606 Guide-it Cas9 Polyclonal Antibody 3 x 100 μl酶试剂盒

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Cas9蛋白质检测抗体
品牌 Code No. 产品名称 包装量 价格(元) 说明书 数量
Clontech 632606 Guide-it Cas9 Polyclonal Antibody 3 x 100 μl ¥8,005 Clontech                      632606           Guide-it Cas9 Polyclonal Antibody            3 x 100 μl Clontech                      632606           Guide-it Cas9 Polyclonal Antibody            3 x 100 μl Clontech                      632606           Guide-it Cas9 Polyclonal Antibody            3 x 100 μl
Clontech 632607 Guide-it Cas9 Polyclonal Antibody 100 μl ¥2,669 Clontech                      632606           Guide-it Cas9 Polyclonal Antibody            3 x 100 μl Clontech                      632606           Guide-it Cas9 Polyclonal Antibody            3 x 100 μl Clontech                      632606           Guide-it Cas9 Polyclonal Antibody            3 x 100 μl
Clontech 632627 Guide-it Cas9 Monoclonal Antibody (Clone TG8C1) 3 × 100 μg ¥6,786 Clontech                      632606           Guide-it Cas9 Polyclonal Antibody            3 x 100 μl Clontech                      632606           Guide-it Cas9 Polyclonal Antibody            3 x 100 μl Clontech                      632606           Guide-it Cas9 Polyclonal Antibody            3 x 100 μl
Clontech 632628 Guide-it Cas9 Monoclonal Antibody (Clone TG8C1) 100 μg ¥2,511 Clontech                      632606           Guide-it Cas9 Polyclonal Antibody            3 x 100 μl Clontech                      632606           Guide-it Cas9 Polyclonal Antibody            3 x 100 μl Clontech                      632606           Guide-it Cas9 Polyclonal Antibody            3 x 100 μl
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Clontech 632606 Guide-it Cas9 Polyclonal Antibody 3 x 100 μl酶试剂盒 – 修饰性PEG

 
 
Clontech                      632606           Guide-it Cas9 Polyclonal Antibody            3 x 100 μl Clontech                      632606           Guide-it Cas9 Polyclonal Antibody            3 x 100 μl Clontech                      632606           Guide-it Cas9 Polyclonal Antibody            3 x 100 μl Clontech                      632606           Guide-it Cas9 Polyclonal Antibody            3 x 100 μl Clontech                      632606           Guide-it Cas9 Polyclonal Antibody            3 x 100 μl
 
 
Cas9蛋白质检测抗体
· 重组Cas9蛋白质检测用单克隆抗体/多克隆抗体
· 多克隆抗体可识别野生型Cas9蛋白质和切口酶突变体(单克隆抗体还未进行测验)
· 高特异性:检测哺乳动物细胞裂解液样品时背景很低
· 高灵敏度:这两种抗体均可检测低于1 ng的重组Cas9蛋白质
 
■ 产品说明:
Takara Bio提供的单克隆抗体和多克隆抗体可用于检测Streptococcus pyogenes来源的重组Cas9蛋白质。
Guide-it Cas9 Monoclonal Antibody (Clone TG8C1)是以Streptococcus pyogenes来源的全长重组Cas9蛋白质为抗原,采用杂交瘤细胞制备生产的小鼠源抗体。用于Western blot检测时,此抗体可以识别细菌和哺乳动物细胞裂解液样品中低于1 ng的重组Cas9蛋白质。
Guide-it Cas9 Polyclonal Antibody是用于检测Streptococcus pyogenes来源的重组Cas9蛋白质的兔源抗体。此抗体可以识别Cas9核酸酶的3种突变体:野生型Cas9蛋白质、Cas9切口酶和Cas9核酸酶-缺陷型突变体。此抗体具备高特异性和高灵敏度,检测哺乳动物细胞样品时其背景很低,用于Western blot检测时可识别低至0.5 ng的重组Cas9蛋白质。
 
Clontech                      632606           Guide-it Cas9 Polyclonal Antibody            3 x 100 μl
图1. Western blot评估Guide-it Cas9 Polyclonal Antibody检测灵敏度
 
0.15-20.0 ng重组Cas9蛋白质(rCas9)电泳后采用Guide-it Cas9 Polyclonal Antibody(1:1,000稀释)进行Western Blot检测。采用HRP-conjugated goat anti-rabbit secondary antibody(1:5,000 稀释)作为二抗,ECL作为检测试剂。HT1080、HEK293细胞裂解液作为阴性对照。
 
Lane 1:HT1080細胞裂解液(3.5×104 cells)
Lane 6:rCas9 protein 1.25 ng
Lane 2:HEK293細胞裂解液(3.5×104 cells)
Lane 7:rCas9 protein 2.5 ng
Lane 3:rCas9 protein 0.15 ng
Lane 8:rCas9 protein 5.0 ng
Lane 4:rCas9 protein 0.30 ng
Lane 9:rCas9 protein 10.0 ng
Lane 5:rCas9 protein 0.625 ng
Lane 10:rCas9 protein 20.0 ng
 
Clontech                      632606           Guide-it Cas9 Polyclonal Antibody            3 x 100 μl
图2.Western blot评估Guide-it Cas9 Polyclonal Antibody检测特异性
 
制备多种未转染细胞和表达Cas9重组蛋白质的HEK 293细胞(CMV启动子)的细胞裂解液,以细胞裂解液为样品通过Western blot方法评估Guide-it Cas9 Polyclonal Antibody检测特异性。1 ng重组Cas9蛋白质(rCas9)作为阳性对照。Guide-it Cas9 Polyclonal Antibody (1:1,000稀释)作为一抗,HRP-conjugated goat anti-rabbit secondary antibody(1:5,000 稀释)作为二抗,ECL作为检测试剂。
 
Lane 1:HepG2细胞裂解液(3.5×104 cells)
Lane 8:裂解缓冲液
Lane 2:CHO-K1细胞裂解液(3.5×104 cells)
Lane 9:rCas9 protein 1 ng
Lane 3:HeLa细胞裂解液(3.5×104 cells)
Lane 10:Cas9表达HEK293细胞裂解液(2.0×103 cells)
Lane 4:MCF7细胞裂解液(3.5×104 cells)
Lane 11:Cas9表达HEK293细胞裂解液(5.0×103 cells)
Lane 5:HEK293细胞裂解液(3.5×104 cells)
Lane 12:Cas9表达HEK293细胞裂解液(1.0×104 cells)
Lane 6:HT1080细胞裂解液(3.5×104 cells)
Lane 13:Cas9表达HEK293细胞裂解液(1.5×104 cells)
Lane 7:NIH3T3细胞裂解液(3.5×104 cells)
 
 
Clontech                      632606           Guide-it Cas9 Polyclonal Antibody            3 x 100 μl
图3. Western blot评估Guide-it Cas9 Monoclonal Antibody (Clone TG8C1) 检测灵敏度
 
A. 1~5 ng重组Cas9蛋白质(rCas9)电泳后采用Guide-it Cas9 Monoclonal Antibody (Clone TG8C1)(1:3,000稀释)进行Western Blot检测。HEK293细胞裂解液作为阴性对照。
B. 以表达Cas9重组蛋白质的HEK 293细胞的细胞裂解液为样品,采用Guide-it Cas9 Monoclonal Antibody (Clone TG8C1)(1:3,000稀释)进行Western Blot检测。HT1080、HEK293细胞裂解液作为阴性对照。
 
■ 保存
-20℃
 
■ 用途
Western blot(1:1,000~1:5,000稀释)
 
 
产品详情请点击:Clontech                      632606           Guide-it Cas9 Polyclonal Antibody            3 x 100 μl