Hampton 蛋白质结晶试剂和耗材介绍

美国Hampton Research公司位于加利福尼亚州，是一家专门从事蛋白质晶体研究的生产商。该公司向全球科研人员提供全面的晶体研究试剂和相关实验耗材，同时提供晶体设计和合成方面的定制服务。Hampton Research公司以其先进的生物大分子结晶技术和广泛实用的产品线，不仅为全世界晶体研究人员的工作带来了便捷，而且也受益于科研人员的回馈不断发展自己，目前已成为晶体研究领域最受信赖的品牌之一。
上海金畔生物作为Hampton research品牌蛋白结晶产品的代理商，我们将竭诚为您服务，欢迎新老客户咨询。

Hampton research主要产品：

美国Hampton Research公司是世界上著名的蛋白质晶体试剂供应商，该公司的产品目录是由上海金畔生物科技有限公司提供，该目录分为12个大类和几十个小类。

Hampton蛋白质结晶试剂及耗材分类

第一类：Crystallization Screens   用于筛选和优化蛋白质/多肽/核酸结晶的一系列经验证的高精配方型溶液

1.PCT Pre-Crystallization Test（PCT预结晶试验）

应用：结晶筛选，确定相应的样品浓度，PCT试验用来预结晶结晶筛选，以确定适当的蛋白质浓度

2. Slice pH（PH切片）

应用：溶解度和结晶筛选

3. Solubility & Stability Screen（溶解度和稳定性的屏幕）

应用：溶解性和稳定性屏幕的设计，以协助识别解决方案的条件，促进蛋白质的溶解性和稳定性，并尽量减少蛋白质沉淀。

4. Index

应用：不同的试剂系统的结晶屏幕的蛋白质复合物，多肽，核酸，水溶性小分子。

5. Crystal Screen（晶体屏幕）

应用：

主屏幕的可溶性肽，蛋白质，核酸，和水溶性小分子。稀疏矩阵的加法屏幕

5.PEG/Ion Screen & PEG/Ion HT Screen 用于可溶性蛋白的结晶

6.PEG/Ion 2 Screen?用于可溶性生物大分子结晶

7.Crystal Screen? & Crystal Screen? HT 用于蛋白质，复合物，缩氨酸，核酸以及水溶性小分子的传统的经典结晶试剂

8.Crystal Screen 2?用于蛋白质，缩氨酸，核酸以及水溶性小分子的结晶

9.Crystal Screen Lite?用于蛋白质，缩氨酸和核酸的结晶试剂

10.Crystal Screen Cryo?用于蛋白质，缩氨酸，核酸的结晶

11.Grid ScreenTM用于蛋白质的结晶，分为七小类。

12.Quik Screen?用于可溶性蛋白的结晶

13.Quik Optimize?快速优化试剂，与Quik Screen? 或 custom screen配套使用

14.Low Ionic Strength Screen?用于完整的单克隆抗体，单克隆抗体片段以及低离子浓度很少溶解的蛋白的结晶

15.Nucleic Acid Mini Screen?用于核酸片段的结晶化

16.Natrix?用于核酸，蛋白核酸聚合物的结晶

17.MembFac?& MembFac? HT用于膜蛋白的结晶

18.Additive Screen?增强或改变样品可溶性的试剂

19.Detergent Screen?去垢剂

20.Heavy Atom Screens重原子结晶试剂

21.Izit Crystal Dye?蛋白染色试剂，用于区分蛋白晶体和盐晶体

第二类：Custom Shop Crystallization Reagents  结晶试剂盒中单独的试剂

1. Solubility & Stability Individual Reagents
2. Slice pH Individual Reagents
3. Ionic Liquid Screen Individual Reagents
4. Proti-Ace & Proti-Ace 2 Individual Reagents
5. PCT Individual Reagents
6. Silver Bullets & Silver Bullets HT Individual Reagents
7. Silver Bullets Bio & Silver Bullets Bio HT Individual Reagents
8. Index • Index HT Individual Reagents
9. PEGRx 1 Individual Reagents
10. PEGRx 2 Individual Reagents
11. SaltRx Individual Reagents
12. PEG/Ion Individual Reagents
13. Crystal Screen Lite Individual Reagents
14. Crystal Screen Cryo Individual Reagents
15. Crystal Screen 2 Cryo Individual Reagents
16. Grid Screen Ammonium Sulfate Individual Reagents
17. Grid Screen PEG 6000 Individual Reagent
18. Grid Screen Sodium Chloride Individual Reagents
19. Grid Screen PEG/LiCl Individual Reagents
20. Quik Screen Individual Reagents
21. Natrix Individual Reagents

第三类：Optimize Reagents 优化试剂

1.Optimize-Polymers聚合体

2.Optimize-Organics (Volatile) 挥发性有机物

3.Optimize-Organics (Non-Volatile)非挥发性的有机物

4.Optimize-Salts 盐类

5.Optimize-Buffers缓冲液

6.Optimize-Solubilizing Agents (NDSB) 溶解试剂NDSB

7.Optimize-Reducing Agent还原剂-TCEP氢氯化物

8.Optimize-Cryoprotectants

9.Optimize-Oils用于结晶的油类

10.Optimize-Silica Hydrogel硅胶

11.Optimize-LM Agarose低熔点琼脂糖

第四类：StockOptions Kits

1. StockOptions Salt
2. StockOptions pH
3. StockOptions Buffer
4. CryoPro

第五类：Crystallization Plates, Hardware & Accessories 结晶耗材

1.24 Well Crystallization Plates 24孔晶体板-分悬滴和坐滴两种

2.48 Well Crystallization Plates 48孔晶体板

3.72 Well Crystallization Plates72孔晶体板

4.96 Well Crystallization Plates 96孔晶体板

5.384 Well Crystallization Plates 384孔晶体板-用于坐滴的结晶

6.Cover Slides & Related Tools

7.Glass Sitting Drop Rods用于坐滴的结晶

8.Micro-Bridges?微桥-坐滴结晶的支撑物

9.Glass Plates – 3 and 9 Well3孔和9孔玻璃板

10.Sealants, Sealing Grease, Oils, Applicator密封油脂，油及其它器具

11.Sealing Films, Tapes, Mats & Covers

12.Microdialysis Buttons, Rods & Membranes微量渗析结晶化

13.Granada Crystallization Box

第六类：Tools, Seeding & Resin（结晶工具、树脂）

1. Tungsten-carbide Glass Cutter
2. X-Acto Gripster Knife
3. Micro-Tools™ Set
4. Micro-Tools II™ Set
5. Forceps
6. Crystal Probe™
7. Crystal Pencil
8. Seed Bead
9. Seeding Tool

第七类：Cryocrystallography

1. CrystalCap HT™ Systems
2. CrystalCap Magnetic™ Systems
3. CrystalCap™ Systems
4. CryoLoops & MicroTubes
5. Cryo Tools
6. Canes, Sleeves, Coders & Boxes
7. Dewars

第八类：Capillary Mounts & Supplies（毛细管座椅支架供应）

第九类：Goniometer Heads & Supplies（测角头及用品）

第十类：Xenon Derivatization（氙气衍生物）

第十一类：Labels & Pens（标签和记录笔）

第十二类：Protein Crystallization Standards（蛋白质结晶的标准）

Hampton代理，Hampton上海代理，Hampton北京代理，Hampton广州代理，Hampton总代理，Hampton一级代理，Hampton特约代理，Hampton现货上海金畔生物科技有限公司Hampton专业代理。

Sigma代理  Abcam代理  CST代理  Santa Cruz代理  Biolegend代理 ebioscience代理  Invitrogen代理   millipore代理  BD流式抗体代理   GeneTe x抗体代理  Novus抗体代理   R&D代理 Biovison代理  Jackson代理  MBL抗体代理  ProSpec抗体代理  Bethyl抗体代理  Antibody Revolution抗体代理  Torrey Pines Biolabs代理  Amresco代理  MPbio代理  Laysan bio代理  NANOCS代理  Avanti代理  wako代理  lumiprobe代理（活性荧光染料）   NEB酶代理  Roche酶代理  Toyobo酶代理   USP代理  EP代理  Dr代理  TRC代理  TCI代理  Reagecon代理 Megazyme代理 Hampton代理（蛋白结晶） whatman代理（滤膜滤纸） GE代理（蛋白纯化） Corning康宁代理  Axygen代理 Falcon代理  NISSUI日水代理 Himedia代理 OXOID代理  BD培养基代理 Ludger代理（糖蛋白分析产品）  Eppendorf代理  Labnet代理  标准品代理 抗体代理 酶试剂代理  培养基代理 耗材代理 Elisa试剂盒代理 。

Hampton 银弹 Silver Bullets

Hampton PEG/Ion • PEG/Ion 2 • PEG/Ion HT

PEG/Ion, developed by Hampton Research, is a crystallization screen designed to evaluate monodisperse, high purity Polyethylene glycol 3,350 and 48 unique salts representing a very complete range of anions and cations frequently used in the crystallization of biological macromolecules. The primary screening variables are PEG, ion type, ionic strength, and pH. More than 60% of the published crystallizations utilized PEG as a primary crystallization reagent and in approximately 50% of those reports, the PEG was combined with an ion as a secondary crystallization reagent. PEG/Ion reagents are formulated without a buffer and are not pH titrated.

PEG/Ion 2 is an extension to the fundamental crystallization strategy in PEG/Ion. PEG/Ion 2 reagents cover the monodisperse, high purity Polyethylene glycol 3,350 and an array of neutralized and pH adjusted organic acids, multivalent ions, a novel Citrate BIS-TRIS propane buffer system and pH (4 – 8.8). The formulation of PEG/Ion 2 was developed at Hampton Research. Each of the 48 reagents in PEG/Ion 2 contains PEG 3,350 as the polymer (precipitant). The concentration of PEG is varied from 12% w/v to 20% w/v depending upon the type and concentration of buffer/salt paired with the polymer. Thirteen of the forty-eight PEG/Ion 2 reagents contain a separate buffer component. The remaining PEG/Ion 2 reagents are buffered by the titrated organic acid salt. Six of these thirteen conditions feature a novel Citric acid BIS-TRIS propane (CBTP) buffer. The CBTP buffer uses Citric acid and BIS-TRIS propane as the acid base pair to create a two component buffer system effective across pH 2.5 to 9.5. The ratio of Citric acid to BIS-TRIS propane determines the solution pH. Thirty-five of the forty-eight PEG/Ion 2 reagents contain a neutralized or pH adjusted organic acid in the presence of the polymer. Neutralized organic acids are highly effective crystallization salts.¹ Four PEG/Ion 2 reagents feature polyvalent cations. Two of these reagents contain cation mixes, saving sample by screening six different cations with only two reagents. Tryptone, a casein digest combinatorial library of peptides, is included in PEG/Ion 2. PEG/Ion 2 reagents 1-30, 42, 45-47 are formulated without a buffer and are not pH titrated.

PEG/Ion contains 48 unique reagents, 10 ml each.

PEG/Ion 2 contains 48 unique reagents, 10 ml each.

PEG/Ion HT contains 1 ml of each reagent from PEG/Ion and PEG/Ion 2 in a single Deep Well block format.

Ready-to-use reagents are sterile filtered and formulated with ultra-pure Type 1 water, using the highest purity salts, polymers, organics and buffers. Individual reagents are available through the Hampton Research Custom Shop.

Crystals of Peptidoglycan Recognition Protein (PGRP) from camel milk grown using the Hampton Research PEG/Ion screen. Sharma, P., Singh, N., Sinha, M., Sharma, S., Perbandt, M., Betzel, C., Kaur, P., Srinivasan, A. & Singh, T.P. All India Institute of Medical Sciences, Department of Biophysics, New Delhi, India. CAT NO NAME DESCRIPTION HR2-126 PEG/Ion Screen 10 ml, tube format HR2-098 PEG/Ion 2 Screen 10 ml, tube format HR2-139 PEG/Ion HT 1 ml, Deep Well block format

Wizard Tube 
The Wizard™ Classic line of random sparse matrix screens is designed to increase your probability of producing crystals during the coarse screening phase when crystallizing biological macromolecules (proteins, nucleic acids, peptides, and combinations thereof).
The Wizard Classic reagents are proven to be a highly effective starting point in the screening of biological macromolecules. The Wizard Classic formulations include a large range of crystallants, buffers, and salts covering a broad range of crystallization space at pH levels from pH 4.5 to pH 10.5.Choose from Wizard Classic 1, 2, 3, or 4 non-overlapping formulations in matrix blocks or tubes.

Wizard Cubic LCP
The tools in the Wizard Cubic LCP (lipidic cubic phase) Kit enable researchers to prepare LCP-type crystallizations by hand. Ideal for low-protein experiments: effective protein volume for a single crystallization experiment is about 80 nanoliters. Wizard Cubic LCP Kit tools work especially well when traditional methods have failed to yield crystals. Lipidic cubic phase has worked well for the crystallization of 7TM membrane proteins (proteins with seven transmembrane helices). Four out of six GPCRs (G-protein coupled receptors), an important membrane protein class, and several microbial 7TM proteins have been crystallized using the LCP approach.

CRYO (I、II)
The Wizard Cryo™ line of random sparse matrix screens is designed for scientists who want to avoid the additional step of optimizing a cryoprotectant condition. Every Wizard Cryo formulation flash-freezes to a clear, amorphous glass in liquid nitrogen or in a cryo-stream at 100K. Crystals can be frozen directly from their growth drops, avoiding the additional step of pre-equilibration with an artificial cryo-condition that can damage the crystal. Eleven different cryocrystallants and sparing use of glycerol ensures a broad sampling of possible cryo conditions. Choose from Wizard Cryo 1 or 2 formulations in tubes or Wizard Cryo 1 and 2 together in a 96-well matrix block.

Hampton 24孔坐滴板 Cryschem Plate

Applications

 Sitting drop crystallization

  Heavy atom soaks

  Seeding

The Cryschem Plate is a 24 well sitting drop plate that is sealed with clear sealing tape. The polystyrene plate is supplied with a raised cover so the plate can also be sealed with vacuum grease and plain glass or plastic cover slides (22 mm diameter). The stackable, 24 well plates have distortion-free, flat bottom wells and a concave sitting drop post in the center of the well for concentric equilibration of the drop with the reagent. Each plate is individually sealed. The top of the plate and each of the 24 wells is a single, flat plane for smooth and easy seal application. Well spacing provides for a large seal area between wells to prevent cross contamination and evaporation.

上海金畔生物作为Hampton蛋白结晶产品的代理商，竭诚为您服务，欢迎新老客户咨询。

Cryschem Plate is large plate footprint with circular reagent reservoir. Cryschem plate approximate size: 15.1 cm x 10.6 cm x 2.2 cm. Approximate well size: 1.6 cm x 1.5 cm. Typical fill volume: 500 to 1,000 µl. Well capacity: 1.5 ml. Maximum drop volume on post: 40 µl. Recommended seal: HR3-511 Crystal Clear Sealing Tape (1.88 inch x 43.7 yard roll, with cutter) or HR4-511 Crystal Clear Sealing Tape (1.88 inch x 60 yard roll, without cutter).

Cryschem M Plate is microplate footprint with circular reagent reservoir. Cryschem M microplate approximate size: 128 mm x 86 mm x 14 mm. Approximate circular reservoir size: 15 mm wide x 9 mm deep. Typical reservoir volume: 500 µl. Well capacity: 1.1 ml. Maximum drop volume on post: 30 µl. Recommended seal: HR3-609 Crystal Clear Sealing Film (100 pack), HR4-521 ClearSeal Film (100 pack) or HR4-506 Crystal Clear Sealing Tape (3 inch x 55 yard roll, without cutter). HR4-508 Crystal Clear Sealing Tape (0.75 inch x 650 inch, with cutter) seals a single row at a time.

Cryschem S Plate is microplate footprint with square reagent reservoir. Cryschem S microplate approximate size: 128 mm x 86 mm x 14 mm. Approximate square reservoir size: 15 mm wide x 9 mm deep. Typical reservoir volume: 500 µl. Well capacity: 1.1 ml. Maximum drop volume on post: 30 µl. Recommended seal: HR3-609 Crystal Clear Sealing Film (100 pack), HR4-521 ClearSeal Film (100 pack) or HR4-506 Crystal Clear Sealing Tape (3 inch x 55 yard roll, without cutter). HR4-508 Crystal Clear Sealing Tape (0.75 inch x 650 inch, with cutter) seals a single row at a time.

Cryschem plate

Sigma代理  Abcam代理  CST代理  Santa Cruz代理  Biolegend代理 ebioscience代理  Invitrogen代理   millipore代理  BD流式抗体代理   GeneTe x抗体代理  Novus抗体代理   R&D代理 Biovison代理  Jackson代理  MBL抗体代理  ProSpec抗体代理  Bethyl抗体代理  Antibody Revolution抗体代理  Torrey Pines Biolabs代理  Amresco代理  MPbio代理  Laysan bio代理  NANOCS代理  Avanti代理  wako代理  lumiprobe代理（活性荧光染料）   NEB酶代理  Roche酶代理  Toyobo酶代理   USP代理  EP代理  Dr代理  TRC代理  TCI代理  Reagecon代理 Megazyme代理 Hampton代理（蛋白结晶） whatman代理（滤膜滤纸） GE代理（蛋白纯化） Corning康宁代理  Axygen代理 Falcon代理  NISSUI日水代理 Himedia代理 OXOID代理  BD培养基代理 Ludger代理（糖蛋白分析产品）  Eppendorf代理  Labnet代理  标准品代理 抗体代理 酶试剂代理  培养基代理 耗材代理 Elisa试剂盒代理 。

Hampton Siliconized Glass Cover Slides

ApplicationsHanging, sitting or sandwich drop crystallization
FeaturesHigh purity of glassSiliconized, hydrophobic glass surface0.22 mm & 0.96 mm glass thicknessThicker glass slides preserve temperature longer12 mm, 18 mm, & 22 mm glass diameterCircle & squareUV Compatible (>85% transmission)
12 x 0.22 mm Circle
18 x 0.22 mm Circle
22 x 0.22 mm Circle & Square

Description
Siliconized glass cover slides allow a droplet to be suspended in a position which provides near optimal conditions for vapor diffusion with the surrounding reservoir solution. These siliconized glass cover slides provide a consistent, high quality finish for crystallization experiments. The hydrophobic surface produces a drop which “stands well” and does not flatten on the glass. The siliconized surface prevents the adhesion of crystals and precipitate onto the glass surface. Use Dow Corning Vacuum Grease (HR3-510), Dow Corning 7 Release Compound (HR3-508), or immersion oil to seal cover slide to plate. Available in 12, 18 or 22 mm diameter circles and 22 mm diameter squares. Available in 0.22 mm or 0.96 mm glass thickness. The 0.96 mm thick slides are virtually unbreakable during crystallization handling.

The following slides are UV transmissable (>85%):
HR3-277 and HR3-279 – 12 mm x 0.22 mm Siliconized circle cover slides
HR3-231 and HR3-233 – 22 mm x 0.22 mm Siliconized circle cover slides
HR3-239 and HR3-241 – 18 mm x 0.22 mm Siliconized circle cover slides
HR3-215 and HR3-217 – 22 mm x 0.22 mm Siliconized square cover slides

Plain Glass Cover Slides
ApplicationsHanging, sitting or sandwich drop crystallization
Features
0.22 mm glass thickness
12 mm glass diameter
Hydrophilic glass surface
Circle & squareDescription
Plain, non-siliconized, hydrophilic glass cover slides. Useful for hanging drop, sitting drop, and sandwich drop vapor diffusion crystallization methods. The hydrophilic surface creates a drop which is more flat than a drop on a siliconized surface. This offers enhanced imaging for very small drops. Use Dow Corning® Vacuum Grease (HR3-510), Dow Corning® 7 Release Compound (HR3-508), or immersion oil to seal cover slide to plate.

HR3-209T 12 mm Plain circle cover slides for Tecan robot 5 oz case (~2,400 slides)

HR3-209T 12 mm Plain circle cover slides for Tecan robot 5 oz case (~2,400 slides)

PEN-VAC Kit
ApplicationsHolding and manipulating small, flat surfaces such as glass cover slides
FeaturesSelf-contained vacuumNo power neededCompactDescription
PEN-VAC is a great tool for handling small, flat surfaced objects such as plain and siliconized cover slides, as well as plastic slides. Simply depress the side button, touch the tip to the object to be lifted and release the button. PEN-VAC creates a totally self-contained vacuum that lifts up to 50 grams for up to one minute. Less than an ounce, the lightweight aluminum body fits in a pocket. No power supply needed. The kit includes one PEN-VAC with 5 3/4″ blue body, (3) angled and (3) straight probes with (2) each 1/8”, 1/4”, 3/8” diameter blue silicone vacuum cups . It is packaged in a clear plastic storage case.

HR3-251  PEN-VAC Kit

Hampton 0.1毫米50号玻璃毛细管

货号：HR6-104
包装：25 pack

• 英文名：0.1 mm Glass Number 50 Capillary
• 品牌：Hampton

Hampton代理–金畔生物

硼硅玻璃50号毛细管壁厚0.01毫米，全长80毫米+ / – 5毫米。提供外径从0.1毫米到2毫米的毛细管，用于X射线数据收集，晶体密度测量和晶体生长实验。毛细管可以用蜡，环氧树脂等密封材料封闭，防止水分和气体。

应用

X射线数据采集

液-液扩散结晶

凝胶针状结晶

特征

50号硼硅玻璃

壁厚 10微米

描述

硼硅玻璃50号毛细管一端呈漏斗形，另一端封闭，壁厚0.01毫米，全长80毫米+/-5毫米，外径从0.1毫米到2毫米。可嵌入，盛放，储存小分子和生物大分子晶体用于X射线数据收集，晶体密度测量和晶体生长实验。毛细管可以用蜡，环氧树脂和其它密封材料密封，防止水分和气体。

请参照“线性吸收系数µcm-1表“，以决定适合的玻璃/石英玻璃毛细管。本表展示的是X射线数据收集时的辐射吸收量。

0.1mm至2.0mm毛细管开口端的基础尺寸为：3.0+/-0.15mm外径 x 0.13+/-0.10 mm壁厚，直径为在毛细管封闭端40毫米处的测量值。

 

货号	产品说明	公差
HR6-104	Size: 0.1 mm – 25 pack	0.1 mm -0.075 / +0.075 mm
HR6-106	Size: 0.2 mm – 25 pack	0.2 mm -0.050 / +0.050 mm
HR6-108	Size: 0.3 mm – 25 pack	0.3 mm -0.050 / +0.100 mm
HR6-110	Size: 0.4 mm – 25 pack	0.4 mm -0.100 / +0.100 mm
HR6-112	Size: 0.5 mm – 25 pack	0.5 mm -0.100 / +0.100 mm
HR6-114	Size: 0.6 mm – 25 pack	0.6 mm -0.100 / +0.100 mm
HR6-116	Size: 0.7 mm – 25 pack	0.7 mm -0.150 / +0.150 mm
HR6-118	Size: 0.8 mm – 25 pack	0.8 mm -0.150 / +0.150 mm
HR6-120	Size: 0.9 mm – 25 pack	0.9 mm -0.150 / +0.150 mm
HR6-122	Size: 1.0 mm – 25 pack	1.0 mm -0.150 / +0.250 mm
HR6-124	Size: 1.5 mm – 25 pack	1.5 mm -0.250 / +0.250 mm
HR6-126	Size: 2.0 mm – 25 pack	2.0 mm -0.250 / +0.250 mm

Hampton 蛋白结晶试剂–Crystal Screen

Crystal Screen • Crystal Screen 2 • Crystal Screen HT

Description
The Crystal Screen and Crystal Screen 2 reagent kits are designed to provide a highly effective and rapid screening method for the crystallization of macromolecules. The screens are simple and practical for finding initial crystallization conditions. The initial crystallization conditions for more than 1,000 proteins, peptides, oligonucleotides, and small molecules have been determined using Crystal Screen.

A highly effective approach to overcome the exhaustive search for suitable crystallization conditions is the use of a sparse matrix method of trial conditions that is biased and selected from known crystallization conditions for macromolecules. The formulation utilized in Crystal Screen and Crystal Screen 2 evaluates 96 unique mixtures of pH, salts, polymers and organics, and their ability to promote crystal growth.

Crystal Screen contains 50 unique reagents, 10 ml each and is based on the sparse matrix formulation first described by Jancarik and Kim in 1991.

Crystal Screen 2, an extension of Crystal Screen, contains 48 unique reagents, 10 ml each and is based on the formulation first described by Cudney et al in 1994.

Crystal Screen HT contains 1 ml each of reagents 1-48 from Crystal Screen and all 48 reagents from Crystal Screen 2 in a single Deep Well block format.

Ready-to-use reagents are sterile filtered and formulated with ultra-pure Type 1 water, using the highest purity salts, polymers, organics and buffers. Individual reagents are available through the Hampton Research Custom Shop.

Crystals of a carbohydrate processing enzyme grown using Hampton Research Crystal Screen. Shirley Roberts, York Structural Biology Laboratory, York, United Kingdom.

Crystal of zRFP574 red fluorescent protein grown using Hampton Research Crystal Screen 2. Pletneva et al. Acta Cryst. D62, 527-532 (2006). Nadya, Russian Academy of Science, Moscow, Russia.

Hampton蛋白结晶工具及仪器

CryoTong™ – Long
ApplicationsCrystal transfer under cryo temperature
FeaturesArtery clamp style closure30 seconds of cryo temperature180 mm length
Description
The CryoTong is a tool used to manually transfer a crystal mounted on a CrystalCap™ from liquid nitrogen to a magnetic base in a goniometer head positioned in a cryogenic stream, and then back to liquid nitrogen. The one-piece, compact CryoTong is available in two lengths. The long CryoTong is approximately 180 mm in overall length. The artery clamp style maintains the CryoTong in the closed position until the clamp is squeezed, which opens the opposing heads. The heads are non-magnetic stainless steel. The handle is magnetic stainless steel. The inside of the head is machined to closely surround the CrystalCap with loop and crystal in place. A small retaining lip is machined into the lower portion of the head to prevent the CrystalCap from slipping out of the CryoTong when the tool is in the closed position. The CryoTong can maintain the temperature of the crystal at -160°C for up to 30 seconds during room temperature crystal transfers. Choose the proper CryoTong size based upon the pin length required by the configuration of the x-ray data collection hardware used. The 18 mm CryoTong (HR5-112) is to be used with the CrystalCap HT and any other CrystalCap configured for 18 mm.HR5-114 Long CryoTong 18 mm, 180° features the head in alignment with the handle of the CryoTong.

Vial Clamp™ – Straight
ApplicationsVial support and manipulation
FeaturesStraight end tipHemostat style closureDescription
The Vial Clamp™ – Straight is a chrome plated, hemostat style tool. It has a tip shaped to hold the storage vial of all the CrystalCap Systems™ straight when the clamp is closed. The clamp can be locked using the hemostat style closure. This clamp makes it easy to dip the storage vial into liquid nitrogen for crystal storage. The complete length of the clamp is straight, with an overall length of 195 mm. When the vial is placed in the clamp, the length of it is positioned perpendicular, 90° to the length of the clamp.

CrystalWand™ Magnetic
ApplicationsCrystalCap Magnetic & CrystalCap Copper Magnetic handling toolDescription
The CrystalWand Magnetic is designed to be used exclusively with the CrystalCap Magnetic, CrystalCap Copper Magnetic, and CrystalCap HT systems during transfer of the caps from the vial to the goniometer head and from the goniometer to the vial. The 6 1/2” (165 mm) long chrome plated steel wand features a plastic housing enclosing a spring tensioned plunger that when depressed, moves a non-magnetic steel platform away from the magnet housed in the end of the wand. This causes the steel CrystalCap to detach from the wand and be placed readily into the vial or the CryoTong™. When the platform is retracted, the wand securely holds the steel CrystalCap Magnetic. The CrystalWand Magnetic 45° (McMiken Tool) offers the same functionality but is bent at a 45° angle at approximately 2.5 cm from the magnet end.HR4-729 CrystalWand Magnetic, StraightHR4-315 CrystalWand Magnetic, 45° (McMiken Tool)

Vial Clamp™ – Curved
ApplicationsVial support & manipulation
FeaturesCurved end tipHemostat style closureDescription
The Vial Clamp – Curved is a chrome plated, hemostat style tool. It has a tip shaped to hold the storage vial of all the CrystalCap Systems at an angle when the clamp is closed. The clamp can be locked using the hemostat style closure. The end of the clamp, where the vial is held, is curved at either a 45°/135° or 110°/ 70° angle. When the vial is placed in the clamp, the length of it is positioned to the clamp at either of those same angles. The overall length of each clamp is the same, 195 mm.HR4-671 Vial Clamp – Curved 45°/135°HR4-672 Vial Clamp – Curved 110°/70°

CrystalCap™ Holder
pplicationsCrystalCap support inside stainless steel dewars
FeaturesAdjustable heightSupports up to 8 CrystalCaps15 ml vial position for liquid propane500 and 1,000 ml versionsDescription
CrystalCap Holders are convenient stands for holding all CrystalCap™ systems in liquid nitrogen dewars during crystal cryogenic procedures. Available in two sizes. Both holders feature spring-clips to secure the CrystalCap Vials in the holder. Both stands have 3 inches of adjustable Z (height) which allows one to raise or lower the CrystalCaps to the appropriate height, depending upon the liquid nitrogen level in the dewar. Each holder is supplied with a socket driver for height adjustment.500 ml version holds five CrystalCaps, two 15 ml vials (for liquid propane procedures) and fits into the 500 ml dewar. 1,000 ml version holds eight CrystalCaps, two 15 ml vials (for liquid propane procedures) and fits into the 1,000 ml dewar.HR4-707 CrystalCap Holder – 500 ml
HR4-706  Replacement Height Adjustment Tool