百日咳毒素Pertussis toxin P2980
名称 品牌 规格 原始型号 价格
Sigma 百日咳毒素Pertussis toxin P2980 EAE模型 多发性硬化模型EAE
Sigma 百日咳毒素Pertussis toxin P2980
Pertussis toxin from Bordetella pertussis Sigma 50UG P2980
Pertussis toxin from Bordetella pertussis Sigma 0.2MG P2980
Pertussis toxin from Bordetella pertussis
buffered aqueous glycerol solution
Synonym: Histamine-sensitizing factor, IAP, Islet Activating Protein, Pertussigen
CAS Number: 70323-44-3
MDL number: MFCD00131841
百日咳毒素Pertussis toxinDescription
Physical form Aseptically filled solution in 50% glycerol, containing 50 mM TRIS, 10 mM glycine, 0.5 M NaCl, pH 7.5
Application The toxin is released from B. pertussis in an inactive form. When the pertussis toxin B oligomer binds to the cell membrane, the S1 subunit of its A protomer becomes activated, perhaps through the action of glutathione and ATP. A protocol for activating pertussis toxin in vitro is given by Kaslow, et al.
百日咳毒素Pertussis toxin Biochem/physiol Actions Pertussis toxin catalyzes the ADP-ribosylation of the α subunits of the heterotrimeric guanine nucleotide regulatory proteins Gi, Go, and Gt. This prevents the G protein heterotrimers from interacting with receptors, thus blocking their coupling and activation. Since the Gα subunits remain in their GDP-bound, inactive state, they are unable to inactivate adenylyl cyclase or open K+ channels.
 
性质
sterility aseptically packaged
form buffered aqueous glycerol solution
storage temp. −20°C
百日咳毒素 Pertussis toxin from Bordetella pertussis
Product Name Pertussis toxin from Bordetella pertussis,
buffered aqueous glycerol solution
Product Number P2980
Product Brand SIGMA
CAS Number 70323-44-3
Storage Temp −20°C
TEST SPECIFICATION
Purity Pass
Migrates as five distinct bands when run on
Polyacrylamide SDS-Urea gels.
Miscellaneous Assay ≤0.33
CHO Cell Assay Lowest concentration of toxin at
which a positive response （clustered growth
pattern） is obtained is
Concentration 0.2 mg/ml
Protein by Bradford