The NEBNext® End Repair Module has been optimized to convert 1 μg – 5μg of fragmented DNA to blunt-

ended DNA having 5´ phosphates, and 3´-hydroxyls. The module is optimized for use with the NEBNext dA-Tailing Module (NEB #E6053), and is part of the original standard DNA library prep workflow.

NEBNext End Repair Enzyme Mix

NEBNext End Repair Reaction Buffer

-20°C

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The NEBNext® End Repair Module has been optimized to convert 1 μg – 5μg of fragmented DNA to blunt-

ended DNA having 5´ phosphates, and 3´-hydroxyls. The module is optimized for use with the NEBNext dA-Tailing Module (NEB #E6053), and is part of the original standard DNA library prep workflow.

 NEBNext End Repair Enzyme Mix

 NEBNext End Repair Reaction Buffer

-20°C
 

请登陆 NEBNextSelector.neb.com 可以使用在线产品选择工具进行产品选择。

The NEBNext® dA-Tailing Module enables incorporation of a non-templated dAMP on the 3´ end of a blunt-ended DNA fragment. The module is optimized for use with the NEBNext dA-Tailing Module (NEB #E6053), and is part of the original standard DNA library prep workflow, which is suitable for 1 – 5 µg of input DNA.

 Klenow Fragment (3´→ 5´ exo–)

 NEBNext dA-Tailing Reaction Buffer

-20°C
 

请登陆 NEBNextSelector.neb.com 可以使用在线产品选择工具进行产品选择。

The NEBNext® dA-Tailing Module enables incorporation of a non-templated dAMP on the 3´ end of a blunt-ended DNA fragment. The module is optimized for use with the NEBNext dA-Tailing Module (NEB #E6053), and is part of the original standard DNA library prep workflow, which is suitable for 1 – 5 µg of input DNA.

 Klenow Fragment (3´→ 5´ exo–)

 NEBNext dA-Tailing Reaction Buffer

-20°C

请登陆 NEBNextSelector.neb.com 可以使用在线产品选择工具进行产品选择。

The NEBNext® Quick Ligation Module has been optimized to ligate efficiently DNA adaptors compatible

with Illumina® sequencing to end-repaired, dA-tailed DNA fragments. The module is optimized for use with the NEBNext End Repair Module (NEB #E6050) and the NEBNext dA-Tailing Module (NEB #E6053) and is part of the original standard DNA library prep workflow, which is suitable for 1 – 5 μg of input DNA.

 Quick T4 DNA Ligase

 NEBNext Quick Ligation Reaction Buffer

-20°C

请登陆 NEBNextSelector.neb.com 可以使用在线产品选择工具进行产品选择。

The NEBNext® Quick Ligation Module has been optimized to ligate efficiently DNA adaptors compatible

with Illumina® sequencing to end-repaired, dA-tailed DNA fragments. The module is optimized for use with the NEBNext End Repair Module (NEB #E6050) and the NEBNext dA-Tailing Module (NEB #E6053) and is part of the original standard DNA library prep workflow, which is suitable for 1 – 5 μg of input DNA.

 Quick T4 DNA Ligase

 NEBNext Quick Ligation Reaction Buffer

-20°C

请登陆 NEBNextSelector.neb.com 可以使用在线产品选择工具进行产品选择。

 简单方便的操作流程 – 产品可用于快速操作和低样本起始量。
 简单方便的剂型 – 试剂盒包含的酶、核苷酸和缓冲液都按照操作所需的浓度和体积提供；
    许多产品都有预混液形式；客户可根据需要个性化选择模块试剂。
 严格的质量监控 – 更多的质量监控确保产品的质量及纯度
 功能验证 – 每种试剂盒和模块都经过相应的文库制备，再通过相应测序平台测序验证（Illumina、
    Ion torrent、454 或 SOLiD）。
 更高性价比   

使用下面的图表，选择最适合 454 文库制备的 NEBNext 产品。 

适用于 454 的 NEBNext DNA 文库制备试剂以试剂盒的形式提供，您可以选择以下两种方法中的任何一种，DNA 快速文库制备法：其中包括末端修复、加 dA 尾、连接接头和片段筛选；也可以选择经典 DNA 文库制备法：其中包括末端修复、连接接头、缺口填充和 ssDNA 筛选步骤。每批次试剂盒都经过构建基因组 DNA 文库，再在 454 测序平台测序进行验证。下表列出了用于 454 DNA 测序文库制备的试剂盒，根据文库制备流程，每个步骤所需试剂也列在表中。适用于 SOLiD 的 NEBNext Small RNA 文库制备试剂以试剂盒形式提供。根据文库制备流程，每个步骤所需试剂也列在表中。