Aminopropargyl ddTTP [5-Propargylamino-2′,3′-dideoxyuridine-5′-triphosphate] CAS 114748-59-3 货号17072-AAT Bioquest荧光染料

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品,欢迎访问AAT Bioquest荧光染料官网了解更多信息。

Aminopropargyl ddTTP [5-Propargylamino-2′,3′-dideoxyuridine-5′-triphosphate] CAS 114748-59-3

Aminopropargyl ddTTP [5-Propargylamino-2′,3′-dideoxyuridine-5′-triphosphate] CAS 114748-59-3

Aminopropargyl ddTTP [5-Propargylamino-2',3'-dideoxyuridine-5'-triphosphate] CAS 114748-59-3     货号17072 货号 17072 存储条件 在零下15度以下保存, 避免光照
规格 10 umoles 价格 11988
Ex (nm) Em (nm)
分子量 593.13 溶剂 DMF
产品详细介绍

简要概述

产品基本信息

货号:17072

产品名称:Aminopropargyl ddTTP [5-Propargylamino-2′,3′-dideoxyuridine-5′-triphosphate]

CAS:114748-59-3 

规格:10 umoles

储存条件:-15℃避光防潮

保质期:24个月

 

产品物理化学光谱特性

分子量:593.13

外观:液体

溶剂:DMF

激发波长(nm):N/A

发射波长(nm):N/A

 

产品介绍

Sanger法是DNA测序中最早最靠谱的方法之一,DNA由四种脱氧核苷酸三磷酸(dNTP)合成。将每个新核苷酸添加到最后的dNTP的3′-OH基团中。可以将二脱氧胸苷三磷酸(ddTTPs)添加到正在生长的DNA链中,但是当它出现时,链延长会停止,因为下一个要连接的核苷酸没有3′-OH。待测序的DNA被制备成单链。该模板DNA带有大量dATP,dGTP,dCTP和dTTP的混合物。加入四种双脱氧核苷酸(ddATP,ddGTP,ddCTP和ddTTP)的混合物,每种混合物均以限量存在,并分别用发不同颜色的荧光的“标签​​”标记。因为所有四个正常核苷酸都存在,所以链延伸正常进行,直到偶然地DNA聚合酶插入ddNTP(而不是正常dNTP)。如果正常核苷酸与双脱氧形式的比率足够高,则在插入ddNTP之前,某些DNA链将成功添加数百个核苷酸,从而终止该过程。在孵育期结束时,片段的长度从最长到最短分离,一个核苷酸之间的差异足以使该链与下一较短和下一较长链分开。当被激光束照射时,四个DDNTP中的每一个都会发出不同的荧光,可以通过自动扫描仪打印输出序列。这些ddATP,ddGTP,ddCTP和ddTTP胺衍生物是开发Sanger测序试剂的重要组成部分。金畔生物是AAT Bioquest的中国代理商,为您提供最优质的Aminopropargyl ddTTP [5-Propargylamino-2′,3′-dideoxyuridine-5′-triphosphate]。 

 

参考文献

Polyadenylated sequencing primers enable complete readability of PCR amplicons analyzed by dideoxynucleotide sequencing
Authors: Beranek, M., Drastikova, M., Petera, J.
Journal: Acta Medica (Hradec Kralove) (2012): 160-4

UV-induced bond modifications in thymine and thymine dideoxynucleotide: structural elucidation of isomers by differential mobility mass spectrometry
Authors: St-Jacques, A., Anichina, J., Schneider, B. B., Covey, T. R., Bohme, D. K.
Journal: Anal Chem (2010): 6163-7

Analysis of processivity of mungbean dideoxynucleotide-sensitive DNA polymerase and detection of the activity and expression of the enzyme in the meristematic and meiotic tissues and following DNA damaging agent
Authors: Roy, S., Choudhury, S. R., Sengupta, D. N.
Journal: Arch Biochem Biophys (2008): 55-65

A dideoxynucleotide-sensitive DNA polymerase activity characterized from endoreduplicating cells of mungbean (Vigna radiata L.) during ontogeny of cotyledons
Authors: Roy, S., Sarkar, S. N., Singh, S. K., Sengupta, D. N.
Journal: FEBS J (2007): 2005-23

Mechanism-based suppression of dideoxynucleotide resistance by K65R human immunodeficiency virus reverse transcriptase using an alpha-boranophosphate nucleoside analogue
Authors: Selmi, B., Boretto, J., Sarfati, S. R., Guerreiro, C., Canard, B.
Journal: J Biol Chem (2001): 48466-72

Synthesis of the first ferrocene-labeled dideoxynucleotide and its use for 3′-redox end-labeling of 5′-modified single-stranded oligonucleotides
Authors: Anne, A., Blanc, B., Moiroux, J.
Journal: Bioconjug Chem (2001): 396-405

Improving dideoxynucleotide-triphosphate utilisation by the hyper-thermophilic DNA polymerase from the archaeon Pyrococcus furiosus
Authors: Evans, S. J., Fogg, M. J., Mamone, A., Davis, M., Pearl, L. H., Connolly, B. A.
Journal: Nucleic Acids Res (2000): 1059-66

Structure-based design of Taq DNA polymerases with improved properties of dideoxynucleotide incorporation
Authors: Li, Y., Mitaxov, V., Waksman, G.
Journal: Proc Natl Acad Sci U S A (1999): 9491-6

Characterization of the native and recombinant catalytic subunit of human DNA polymerase gamma: identification of residues critical for exonuclease activity and dideoxynucleotide sensitivity
Authors: Longley, M. J., Ropp, P. A., Lim, S. E., Copel and W. C.
Journal: Biochemistry (1998): 10529-39

Comparative performance of high-density oligonucleotide sequencing and dideoxynucleotide sequencing of HIV type 1 pol from clinical samples
Authors: Gunthard, H. F., Wong, J. K., Ignacio, C. C., Havlir, D. V., Richman, D. D.
Journal: AIDS Res Hum Retroviruses (1998): 869-76

说明书
Aminopropargyl ddTTP [5-Propargylamino-2′,3′-dideoxyuridine-5′-triphosphate] CAS 114748-59-3 .pdf

ddTTP [2′,3′-Dideoxythymidine-5′-triphosphate] 货号17208-AAT Bioquest荧光染料

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品,欢迎访问AAT Bioquest荧光染料官网了解更多信息。

ddTTP [2′,3′-Dideoxythymidine-5′-triphosphate]

ddTTP [2′,3′-Dideoxythymidine-5′-triphosphate]

ddTTP [2',3'-Dideoxythymidine-5'-triphosphate]    货号17208 货号 17208 存储条件 在零下15度以下保存, 避免光照
规格 1 umole 价格 2472
Ex (nm) Em (nm)
分子量 466.17 溶剂 Water
产品详细介绍

简要概述

产品基本信息

货号:17208

产品名称:ddTTP [2′,3′-Dideoxythymidine-5′-triphosphate]

规格:1 umole

储存条件:-15℃避光防潮

保质期:24个月

 

产品物理化学光谱特性

分子量:466.17

溶剂:水

 

产品介绍

Sanger测序,也称为链终止法,是一种基于DNA聚合酶选择性掺入链终止双脱氧核苷酸(ddNTPs)的DNA测序技术。它是由Frederick Sanger及其同事于1977年开发的。尽管新的NGS技术由于其较高的通量能力和较低的每份样品成本而在临床研究实验室中变得很普遍,但Sanger测序的准确度仍为99.99%,仍然是临床的“黄金标准”研究排序。 dd-TTP是执行Sanger测序的四个关键ddNTP组件之一。

 

参考文献

Strong positional preference in the interaction of LNA oligonucleotides with DNA polymerase and proofreading exonuclease activities: implications for genotyping assays.
Authors: Di Giusto, Daniel A and King, Garry C
Journal: Nucleic acids research (2004): e32

Detection and genotyping of human papillomavirus DNA in cervical cancer tissues with fluorescence polarization.
Authors: Gao, Yan-E and Zhang, Ju and Wu, Jing and Chen, Zhong-Can and Yan, Xiao-Jun
Journal: Sheng wu hua xue yu sheng wu wu li xue bao Acta biochimica et biophysica Sinica (2003): 1029-34

Dye structure affects Taq DNA polymerase terminator selectivity.
Authors: Brandis, J W
Journal: Nucleic acids research (1999): 1912-8

Synergistic inhibition of HIV-1 reverse transcriptase by combinations of chain-terminating nucleotides.
Authors: Villahermosa, M L and Martinez-Irujo, J J and Cabodevilla, F and Santiago, E
Journal: Biochemistry (1997): 13223-31

Characterization of the p68/p58 heterodimer of human immunodeficiency virus type 2 reverse transcriptase.
Authors: Fan, N and Rank, K B and Poppe, S M and Tarpley, W G and Sharma, S K
Journal: Biochemistry (1996): 1911-7

Mechanism of inhibition of DNA synthesis by 1-beta-D-arabinofuranosyl-E-5-(2-bromovinyl)uracil.
Authors: Suzutani, T and Machida, H and Honess, R W
Journal: Microbiology and immunology (1993): 511-3

Expression of enzymatically active reverse transcriptase of simian immunodeficiency virus in bacteria: sensitivity to nucleotide analogue inhibitors.
Authors: Prasad, V R and Myrick, K and Haseltine, W and Goff, S P
Journal: Virology (1990): 896-900

Wheat embryo DNA polymerase A reverse transcribes natural and synthetic RNA templates. Biochemical characterization and comparison with animal DNA polymerase gamma and retroviral reverse transcriptase.
Authors: Laquel, P and Sallafranque-Andreola, M and Tarrago-Litvak, L and Castroviejo, M and Litvak, S
Journal: Biochimica et biophysica acta (1990): 139-48

3′-Deoxy-3′-fluorothymidinetriphosphate: inhibitor and terminator of DNA synthesis catalysed by DNA polymerase beta, terminal deoxynucleotidyl transferase and DNA polymerase I.
Authors: Matthes, E and Lehmann, C and Drescher, B and Büttner, W and Langen, P
Journal: Biomedica biochimica acta (1985): K63-73

A fidelity assay using “dideoxy” DNA sequencing: a measurement of sequence dependence and frequency of forming 5-bromouracil X guanine base mispairs.
Authors: Lasken, R S and Goodman, M F
Journal: Proceedings of the National Academy of Sciences of the United States of America (1985): 1301-5

说明书
ddTTP [2′,3′-Dideoxythymidine-5′-triphosphate].pdf