Clontech 632635 Guide-it sgRNA In Vitro Transcription Kit 50 Rxns ¥8,771 ¥7,017 Clontech 632636 Guide-it Complete sgRNA Screening System 50 Rxns ¥10,701 ¥8,561 Clontech 632639 Guide-it sgRNA Screening Kit 50 Rxns ¥4,810 ¥3,848 Clontech 632638 Guide-it IVT RNA Clean-Up Kit 50 Rxns酶试剂盒

上海金畔生物科技有限公司代理Takara Clontech酶试剂盒全线产品,欢迎访问官网了解更多产品信息和订购。

sgRNA体外转录和筛选
品牌 Code No. 产品名称 包装量 价格(元) 说明书 数量
Clontech 632635 Guide-it sgRNA In Vitro Transcription Kit 50 Rxns ¥8,771
¥7,017
Clontech                      632635           Guide-it sgRNA In Vitro Transcription Kit            50 Rxns            ¥8,771 ¥7,017                          Clontech                      632636           Guide-it Complete sgRNA Screening System            50 Rxns            ¥10,701 ¥8,561                          Clontech                      632639           Guide-it sgRNA Screening Kit            50 Rxns            ¥4,810 ¥3,848                          Clontech                      632638           Guide-it IVT RNA Clean-Up Kit            50 Rxns Clontech                      632635           Guide-it sgRNA In Vitro Transcription Kit            50 Rxns            ¥8,771 ¥7,017                          Clontech                      632636           Guide-it Complete sgRNA Screening System            50 Rxns            ¥10,701 ¥8,561                          Clontech                      632639           Guide-it sgRNA Screening Kit            50 Rxns            ¥4,810 ¥3,848                          Clontech                      632638           Guide-it IVT RNA Clean-Up Kit            50 Rxns Clontech                      632635           Guide-it sgRNA In Vitro Transcription Kit            50 Rxns            ¥8,771 ¥7,017                          Clontech                      632636           Guide-it Complete sgRNA Screening System            50 Rxns            ¥10,701 ¥8,561                          Clontech                      632639           Guide-it sgRNA Screening Kit            50 Rxns            ¥4,810 ¥3,848                          Clontech                      632638           Guide-it IVT RNA Clean-Up Kit            50 Rxns
Clontech 632636 Guide-it Complete sgRNA Screening System 50 Rxns ¥10,701
¥8,561
Clontech                      632635           Guide-it sgRNA In Vitro Transcription Kit            50 Rxns            ¥8,771 ¥7,017                          Clontech                      632636           Guide-it Complete sgRNA Screening System            50 Rxns            ¥10,701 ¥8,561                          Clontech                      632639           Guide-it sgRNA Screening Kit            50 Rxns            ¥4,810 ¥3,848                          Clontech                      632638           Guide-it IVT RNA Clean-Up Kit            50 Rxns Clontech                      632635           Guide-it sgRNA In Vitro Transcription Kit            50 Rxns            ¥8,771 ¥7,017                          Clontech                      632636           Guide-it Complete sgRNA Screening System            50 Rxns            ¥10,701 ¥8,561                          Clontech                      632639           Guide-it sgRNA Screening Kit            50 Rxns            ¥4,810 ¥3,848                          Clontech                      632638           Guide-it IVT RNA Clean-Up Kit            50 Rxns Clontech                      632635           Guide-it sgRNA In Vitro Transcription Kit            50 Rxns            ¥8,771 ¥7,017                          Clontech                      632636           Guide-it Complete sgRNA Screening System            50 Rxns            ¥10,701 ¥8,561                          Clontech                      632639           Guide-it sgRNA Screening Kit            50 Rxns            ¥4,810 ¥3,848                          Clontech                      632638           Guide-it IVT RNA Clean-Up Kit            50 Rxns
Clontech 632639 Guide-it sgRNA Screening Kit 50 Rxns ¥4,810
¥3,848
Clontech                      632635           Guide-it sgRNA In Vitro Transcription Kit            50 Rxns            ¥8,771 ¥7,017                          Clontech                      632636           Guide-it Complete sgRNA Screening System            50 Rxns            ¥10,701 ¥8,561                          Clontech                      632639           Guide-it sgRNA Screening Kit            50 Rxns            ¥4,810 ¥3,848                          Clontech                      632638           Guide-it IVT RNA Clean-Up Kit            50 Rxns Clontech                      632635           Guide-it sgRNA In Vitro Transcription Kit            50 Rxns            ¥8,771 ¥7,017                          Clontech                      632636           Guide-it Complete sgRNA Screening System            50 Rxns            ¥10,701 ¥8,561                          Clontech                      632639           Guide-it sgRNA Screening Kit            50 Rxns            ¥4,810 ¥3,848                          Clontech                      632638           Guide-it IVT RNA Clean-Up Kit            50 Rxns Clontech                      632635           Guide-it sgRNA In Vitro Transcription Kit            50 Rxns            ¥8,771 ¥7,017                          Clontech                      632636           Guide-it Complete sgRNA Screening System            50 Rxns            ¥10,701 ¥8,561                          Clontech                      632639           Guide-it sgRNA Screening Kit            50 Rxns            ¥4,810 ¥3,848                          Clontech                      632638           Guide-it IVT RNA Clean-Up Kit            50 Rxns
Clontech 632638 Guide-it IVT RNA Clean-Up Kit 50 Rxns ¥4,439 Clontech                      632635           Guide-it sgRNA In Vitro Transcription Kit            50 Rxns            ¥8,771 ¥7,017                          Clontech                      632636           Guide-it Complete sgRNA Screening System            50 Rxns            ¥10,701 ¥8,561                          Clontech                      632639           Guide-it sgRNA Screening Kit            50 Rxns            ¥4,810 ¥3,848                          Clontech                      632638           Guide-it IVT RNA Clean-Up Kit            50 Rxns Clontech                      632635           Guide-it sgRNA In Vitro Transcription Kit            50 Rxns            ¥8,771 ¥7,017                          Clontech                      632636           Guide-it Complete sgRNA Screening System            50 Rxns            ¥10,701 ¥8,561                          Clontech                      632639           Guide-it sgRNA Screening Kit            50 Rxns            ¥4,810 ¥3,848                          Clontech                      632638           Guide-it IVT RNA Clean-Up Kit            50 Rxns Clontech                      632635           Guide-it sgRNA In Vitro Transcription Kit            50 Rxns            ¥8,771 ¥7,017                          Clontech                      632636           Guide-it Complete sgRNA Screening System            50 Rxns            ¥10,701 ¥8,561                          Clontech                      632639           Guide-it sgRNA Screening Kit            50 Rxns            ¥4,810 ¥3,848                          Clontech                      632638           Guide-it IVT RNA Clean-Up Kit            50 Rxns

*红字为促销价格,促销时间: 2022年9月1日-2022年10月31日

收藏产品 加入购物车

Sgrna – 修饰性PEG

 
 
          基因敲除    
          Clontech                      632635           Guide-it sgRNA In Vitro Transcription Kit            50 Rxns            ¥8,771 ¥7,017                          Clontech                      632636           Guide-it Complete sgRNA Screening System            50 Rxns            ¥10,701 ¥8,561                          Clontech                      632639           Guide-it sgRNA Screening Kit            50 Rxns            ¥4,810 ¥3,848                          Clontech                      632638           Guide-it IVT RNA Clean-Up Kit            50 Rxns Clontech                      632635           Guide-it sgRNA In Vitro Transcription Kit            50 Rxns            ¥8,771 ¥7,017                          Clontech                      632636           Guide-it Complete sgRNA Screening System            50 Rxns            ¥10,701 ¥8,561                          Clontech                      632639           Guide-it sgRNA Screening Kit            50 Rxns            ¥4,810 ¥3,848                          Clontech                      632638           Guide-it IVT RNA Clean-Up Kit            50 Rxns Clontech                      632635           Guide-it sgRNA In Vitro Transcription Kit            50 Rxns            ¥8,771 ¥7,017                          Clontech                      632636           Guide-it Complete sgRNA Screening System            50 Rxns            ¥10,701 ¥8,561                          Clontech                      632639           Guide-it sgRNA Screening Kit            50 Rxns            ¥4,810 ¥3,848                          Clontech                      632638           Guide-it IVT RNA Clean-Up Kit            50 Rxns
Clontech                      632635           Guide-it sgRNA In Vitro Transcription Kit            50 Rxns            ¥8,771 ¥7,017                          Clontech                      632636           Guide-it Complete sgRNA Screening System            50 Rxns            ¥10,701 ¥8,561                          Clontech                      632639           Guide-it sgRNA Screening Kit            50 Rxns            ¥4,810 ¥3,848                          Clontech                      632638           Guide-it IVT RNA Clean-Up Kit            50 Rxns Clontech                      632635           Guide-it sgRNA In Vitro Transcription Kit            50 Rxns            ¥8,771 ¥7,017                          Clontech                      632636           Guide-it Complete sgRNA Screening System            50 Rxns            ¥10,701 ¥8,561                          Clontech                      632639           Guide-it sgRNA Screening Kit            50 Rxns            ¥4,810 ¥3,848                          Clontech                      632638           Guide-it IVT RNA Clean-Up Kit            50 Rxns Clontech                      632635           Guide-it sgRNA In Vitro Transcription Kit            50 Rxns            ¥8,771 ¥7,017                          Clontech                      632636           Guide-it Complete sgRNA Screening System            50 Rxns            ¥10,701 ¥8,561                          Clontech                      632639           Guide-it sgRNA Screening Kit            50 Rxns            ¥4,810 ¥3,848                          Clontech                      632638           Guide-it IVT RNA Clean-Up Kit            50 Rxns            
  基因敲入        
          Clontech                      632635           Guide-it sgRNA In Vitro Transcription Kit            50 Rxns            ¥8,771 ¥7,017                          Clontech                      632636           Guide-it Complete sgRNA Screening System            50 Rxns            ¥10,701 ¥8,561                          Clontech                      632639           Guide-it sgRNA Screening Kit            50 Rxns            ¥4,810 ¥3,848                          Clontech                      632638           Guide-it IVT RNA Clean-Up Kit            50 Rxns        
 
 
sgRNA的体外转录和筛选试剂盒
In Vitro Transcription and Screening Kits for sgRNA
我们的Guide-it in vitro transcription and screening kits for sgRNA 包括四个试剂盒,用于CRISPR/Cas9 研究中单链向导RNAs(single guide RNAs ,sgRNAs)的简单制备、清理和评价。每个试剂盒提供足够 50次反应的材料。
· Guide-it sgRNA In Vitro Transcription Kit (Code No. 632635) 被用于通过体外转录制备大量的 sgRNAs。这个试剂盒同样包括Guide-it IVT RNA Clean-Up Kit (Code No. 632638), 被用于快速纯化参与体外转录(vitro transcription,IVT) 反应的sgRNA。从这个试剂盒纯化后的 sgRNA 可以直接被用于转染、电穿孔以及体外剪切实验。
· Guide-it sgRNA Screening Kit (Code No. 632639) 可以在Cas9介导的基因编辑相关研究前,先在体外检测不同sgRNAs的效率,这样就不必浪费时间将无效的sgRNAs转入您的细胞了。使用这个试剂盒,首先通过PCR产生包含sgRNA靶点的模板,随后再与您想测试的sgRNA以及重组Cas9核酸酶结合。Cas9 核酸酶剪切模板的效率可以通过使用琼脂糖凝胶电泳检测。
· Guide-it Complete sgRNA Screening System (Code No. 632636) 包含以上提到的全部试剂盒组分,可以被用来合成和检测sgRNAs效率。该试剂盒包含了用于体外转录和纯化sgRNAs,以及检测重组Cas9核酸酶对PCR靶向片段剪切效率的所有试剂。
 
■ 产品特点
· 共有4个试剂盒,可用于Cas9介导的基因编辑研究中sgRNA (single guide RNA)的简单制备、清洁以及效率评价。
· Lenti-X Packaging Single Shots易于产生高滴度病毒。
· 使用Guide-it sgRNA In Vitro Transcription Kit (Code No. 632635; 包括Guide-it IVT RNA Clean-Up Kit, Code No. 632638)体外转录和纯化大量sgRNA,用于转染或体外筛选。
· 使用Guide-it IVT RNA Clean-Up Kit (Code No. 632638) 对IVT 反应后sgRNA的快速纯化,直接用于转染、电穿孔以及体外剪切试验。
· Guide-it sgRNA Screening Kit (Code No. 632639)包含高质量的重组Cas9蛋白质。
· Guide-it Complete sgRNA Screening System (Code No. 632636)是Guide-it sgRNA In Vitro Transcription Kit (Code No. 632635) 和 Guide-it sgRNA Screening Kit (Code No. 632639)的组合,允许您进行sgRNA制备和对预设靶点体外剪切能力的测试。
 
■ 实验例
Clontech                      632635           Guide-it sgRNA In Vitro Transcription Kit            50 Rxns            ¥8,771 ¥7,017                          Clontech                      632636           Guide-it Complete sgRNA Screening System            50 Rxns            ¥10,701 ¥8,561                          Clontech                      632639           Guide-it sgRNA Screening Kit            50 Rxns            ¥4,810 ¥3,848                          Clontech                      632638           Guide-it IVT RNA Clean-Up Kit            50 Rxns
图1. Guide-it sgRNA In Vitro Transcription and Screening System试剂盒组成和操作流程
 
Clontech                      632635           Guide-it sgRNA In Vitro Transcription Kit            50 Rxns            ¥8,771 ¥7,017                          Clontech                      632636           Guide-it Complete sgRNA Screening System            50 Rxns            ¥10,701 ¥8,561                          Clontech                      632639           Guide-it sgRNA Screening Kit            50 Rxns            ¥4,810 ¥3,848                          Clontech                      632638           Guide-it IVT RNA Clean-Up Kit            50 Rxns
图2. 采用Guide-it试剂盒制备高产量single guide RNA (sgRNA)和重组Cas9蛋白质剪切靶位点检测sgRNA剪切效率操作步骤概要
 
Clontech                      632635           Guide-it sgRNA In Vitro Transcription Kit            50 Rxns            ¥8,771 ¥7,017                          Clontech                      632636           Guide-it Complete sgRNA Screening System            50 Rxns            ¥10,701 ¥8,561                          Clontech                      632639           Guide-it sgRNA Screening Kit            50 Rxns            ¥4,810 ¥3,848                          Clontech                      632638           Guide-it IVT RNA Clean-Up Kit            50 Rxns
图3. Guide-it试剂和同类产品所制备的sgRNA品质比较
采用Guide-it sgRNA In Vitro Transcription Kit制备的所有sgRNAs均为单一条带,而其他公司同类产品制备的sgRNAs均可观察到杂带,因此Guide-it试剂盒所制备的sgRNAs品质更高。
* 比较结果来自于Takara Bio USA, Inc.
 
Clontech                      632635           Guide-it sgRNA In Vitro Transcription Kit            50 Rxns            ¥8,771 ¥7,017                          Clontech                      632636           Guide-it Complete sgRNA Screening System            50 Rxns            ¥10,701 ¥8,561                          Clontech                      632639           Guide-it sgRNA Screening Kit            50 Rxns            ¥4,810 ¥3,848                          Clontech                      632638           Guide-it IVT RNA Clean-Up Kit            50 Rxns
图4. 以不同基因为靶标制备的sgRNA产量和品质比较
采用Guide-it Complete sgRNA Screening System以多个基因为靶基因制备sgRNA。
Panel A. 将20 μl sgRNA体外转录(IVT)反应液置于37°C孵育4 hr,采用NanoDrop分光光度计检测所制备产生的sgRNA产量。
Panel B. 采用安捷伦生物分析仪分析分析每一个sgRNA IVT反应液,检测sgRNA质量。
 
■ 产品组份
Guide-it sgRNA In Vitro Transcription Kit (Code No. 632635; 50 rxns)
· 1 each Guide-it sgRNA In Vitro Transcription Components v2 (Code No. 632637; 50 rxns; Not sold separately)
    ○ 50 μl Guide-it Scaffold Template (1 ng/μl)
    ○ 350 μl Guide-it In Vitro Transcription Buffer
    ○ 1 ml RNase Free Water
    ○ 500 μl RNase Free Water
    ○ 150 μl Guide-it T7 Polymerase Mix (33 U/μl)
    ○ 625 μl PrimeSTAR Max Premix (2X)
    ○ 100 μl Recombinant DNase I (RNase-Free) (5 U/μl)
· 1 each Guide-it IVT RNA Clean-Up Kit (Code No. 632638; 50 rxns)
    ○ 13 ml RNase Free Water
    ○ 3 ml IVT Binding Buffer
    ○ 50 IVT RNA Clean-Up Spin Columns
    ○ 2 x 6 ml IVT Wash Buffer
    ○ 50 Collection Tubes (2 ml)

Guide-it sgRNA Clean-Up Kit (Code No. 632638; 50 rxns)
· 13 ml RNase Free Water
· 3 ml IVT Binding Buffer
· 50 IVT RNA Clean-Up Spin Columns
· 2 x 6 ml IVT Wash Buffer
· 50 Collection Tubes (2 ml)

Guide-it sgRNA Screening Kit (Code No. 632639; 50 rxns)
· 25 μl Guide-it Recombinant Cas9 Nuclease (500 ng/μl)
· 50 μl 15X Cas9 Reaction Buffer
· 50 μl 15X BSA
· 5 μl Control sgRNA (50 ng/μl)
· 25 μl Control Fragment (20 ng/μl)
· 50 μl Terra PCR Direct Polymerase Mix (1.25 U/μl)
· 1.25 ml 2X Terra PCR Direct Buffer (with Mg2+, dNTP)
· 5 ml RNase Free Water
· 5 ml Extraction Buffer 1
· 0.5 ml Extraction Buffer 2

Guide-it Complete sgRNA Screening System (Code No. 632636; 50 rxns)
· Guide-it sgRNA In Vitro Transcription Kit (Code No. 632635)
· Guide-it sgRNA Screening Kit (Code No. 632639)

 
 
 
■ 关联资料
点击查看Guide-it sgRNA体外转录和筛选系统宣传页 Clontech                      632635           Guide-it sgRNA In Vitro Transcription Kit            50 Rxns            ¥8,771 ¥7,017                          Clontech                      632636           Guide-it Complete sgRNA Screening System            50 Rxns            ¥10,701 ¥8,561                          Clontech                      632639           Guide-it sgRNA Screening Kit            50 Rxns            ¥4,810 ¥3,848                          Clontech                      632638           Guide-it IVT RNA Clean-Up Kit            50 Rxns
 
 
产品详情请点击:Clontech                      632635           Guide-it sgRNA In Vitro Transcription Kit            50 Rxns            ¥8,771 ¥7,017                          Clontech                      632636           Guide-it Complete sgRNA Screening System            50 Rxns            ¥10,701 ¥8,561                          Clontech                      632639           Guide-it sgRNA Screening Kit            50 Rxns            ¥4,810 ¥3,848                          Clontech                      632638           Guide-it IVT RNA Clean-Up Kit            50 Rxns
 

sgRNA体外转录和筛选酶试剂盒Takara Clontech

上海金畔生物科技有限公司代理Takara Clontech酶试剂盒全线产品,欢迎访问官网了解更多产品信息和订购。

sgRNA体外转录和筛选
品牌 Code No. 产品名称 包装量 价格(元) 说明书 数量
Clontech 632635 Guide-it sgRNA In Vitro Transcription Kit 50 Rxns ¥8,771
¥7,017
sgRNA体外转录和筛选 sgRNA体外转录和筛选 sgRNA体外转录和筛选
Clontech 632636 Guide-it Complete sgRNA Screening System 50 Rxns ¥10,701
¥8,561
sgRNA体外转录和筛选 sgRNA体外转录和筛选 sgRNA体外转录和筛选
Clontech 632639 Guide-it sgRNA Screening Kit 50 Rxns ¥4,810
¥3,848
sgRNA体外转录和筛选 sgRNA体外转录和筛选 sgRNA体外转录和筛选
Clontech 632638 Guide-it IVT RNA Clean-Up Kit 50 Rxns ¥4,439 sgRNA体外转录和筛选 sgRNA体外转录和筛选 sgRNA体外转录和筛选

*红字为促销价格,促销时间: 2022年9月1日-2022年10月31日

收藏产品 加入购物车

Sgrna – 修饰性PEG

 
 
          基因敲除    
          sgRNA体外转录和筛选 sgRNA体外转录和筛选 sgRNA体外转录和筛选
sgRNA体外转录和筛选 sgRNA体外转录和筛选 sgRNA体外转录和筛选            
  基因敲入        
          sgRNA体外转录和筛选        
 
 
sgRNA的体外转录和筛选试剂盒
In Vitro Transcription and Screening Kits for sgRNA
我们的Guide-it in vitro transcription and screening kits for sgRNA 包括四个试剂盒,用于CRISPR/Cas9 研究中单链向导RNAs(single guide RNAs ,sgRNAs)的简单制备、清理和评价。每个试剂盒提供足够 50次反应的材料。
· Guide-it sgRNA In Vitro Transcription Kit (Code No. 632635) 被用于通过体外转录制备大量的 sgRNAs。这个试剂盒同样包括Guide-it IVT RNA Clean-Up Kit (Code No. 632638), 被用于快速纯化参与体外转录(vitro transcription,IVT) 反应的sgRNA。从这个试剂盒纯化后的 sgRNA 可以直接被用于转染、电穿孔以及体外剪切实验。
· Guide-it sgRNA Screening Kit (Code No. 632639) 可以在Cas9介导的基因编辑相关研究前,先在体外检测不同sgRNAs的效率,这样就不必浪费时间将无效的sgRNAs转入您的细胞了。使用这个试剂盒,首先通过PCR产生包含sgRNA靶点的模板,随后再与您想测试的sgRNA以及重组Cas9核酸酶结合。Cas9 核酸酶剪切模板的效率可以通过使用琼脂糖凝胶电泳检测。
· Guide-it Complete sgRNA Screening System (Code No. 632636) 包含以上提到的全部试剂盒组分,可以被用来合成和检测sgRNAs效率。该试剂盒包含了用于体外转录和纯化sgRNAs,以及检测重组Cas9核酸酶对PCR靶向片段剪切效率的所有试剂。
 
■ 产品特点
· 共有4个试剂盒,可用于Cas9介导的基因编辑研究中sgRNA (single guide RNA)的简单制备、清洁以及效率评价。
· Lenti-X Packaging Single Shots易于产生高滴度病毒。
· 使用Guide-it sgRNA In Vitro Transcription Kit (Code No. 632635; 包括Guide-it IVT RNA Clean-Up Kit, Code No. 632638)体外转录和纯化大量sgRNA,用于转染或体外筛选。
· 使用Guide-it IVT RNA Clean-Up Kit (Code No. 632638) 对IVT 反应后sgRNA的快速纯化,直接用于转染、电穿孔以及体外剪切试验。
· Guide-it sgRNA Screening Kit (Code No. 632639)包含高质量的重组Cas9蛋白质。
· Guide-it Complete sgRNA Screening System (Code No. 632636)是Guide-it sgRNA In Vitro Transcription Kit (Code No. 632635) 和 Guide-it sgRNA Screening Kit (Code No. 632639)的组合,允许您进行sgRNA制备和对预设靶点体外剪切能力的测试。
 
■ 实验例
sgRNA体外转录和筛选
图1. Guide-it sgRNA In Vitro Transcription and Screening System试剂盒组成和操作流程
 
sgRNA体外转录和筛选
图2. 采用Guide-it试剂盒制备高产量single guide RNA (sgRNA)和重组Cas9蛋白质剪切靶位点检测sgRNA剪切效率操作步骤概要
 
sgRNA体外转录和筛选
图3. Guide-it试剂和同类产品所制备的sgRNA品质比较
采用Guide-it sgRNA In Vitro Transcription Kit制备的所有sgRNAs均为单一条带,而其他公司同类产品制备的sgRNAs均可观察到杂带,因此Guide-it试剂盒所制备的sgRNAs品质更高。
* 比较结果来自于Takara Bio USA, Inc.
 
sgRNA体外转录和筛选
图4. 以不同基因为靶标制备的sgRNA产量和品质比较
采用Guide-it Complete sgRNA Screening System以多个基因为靶基因制备sgRNA。
Panel A. 将20 μl sgRNA体外转录(IVT)反应液置于37°C孵育4 hr,采用NanoDrop分光光度计检测所制备产生的sgRNA产量。
Panel B. 采用安捷伦生物分析仪分析分析每一个sgRNA IVT反应液,检测sgRNA质量。
 
■ 产品组份
Guide-it sgRNA In Vitro Transcription Kit (Code No. 632635; 50 rxns)
· 1 each Guide-it sgRNA In Vitro Transcription Components v2 (Code No. 632637; 50 rxns; Not sold separately)
    ○ 50 μl Guide-it Scaffold Template (1 ng/μl)
    ○ 350 μl Guide-it In Vitro Transcription Buffer
    ○ 1 ml RNase Free Water
    ○ 500 μl RNase Free Water
    ○ 150 μl Guide-it T7 Polymerase Mix (33 U/μl)
    ○ 625 μl PrimeSTAR Max Premix (2X)
    ○ 100 μl Recombinant DNase I (RNase-Free) (5 U/μl)
· 1 each Guide-it IVT RNA Clean-Up Kit (Code No. 632638; 50 rxns)
    ○ 13 ml RNase Free Water
    ○ 3 ml IVT Binding Buffer
    ○ 50 IVT RNA Clean-Up Spin Columns
    ○ 2 x 6 ml IVT Wash Buffer
    ○ 50 Collection Tubes (2 ml)

Guide-it sgRNA Clean-Up Kit (Code No. 632638; 50 rxns)
· 13 ml RNase Free Water
· 3 ml IVT Binding Buffer
· 50 IVT RNA Clean-Up Spin Columns
· 2 x 6 ml IVT Wash Buffer
· 50 Collection Tubes (2 ml)

Guide-it sgRNA Screening Kit (Code No. 632639; 50 rxns)
· 25 μl Guide-it Recombinant Cas9 Nuclease (500 ng/μl)
· 50 μl 15X Cas9 Reaction Buffer
· 50 μl 15X BSA
· 5 μl Control sgRNA (50 ng/μl)
· 25 μl Control Fragment (20 ng/μl)
· 50 μl Terra PCR Direct Polymerase Mix (1.25 U/μl)
· 1.25 ml 2X Terra PCR Direct Buffer (with Mg2+, dNTP)
· 5 ml RNase Free Water
· 5 ml Extraction Buffer 1
· 0.5 ml Extraction Buffer 2

Guide-it Complete sgRNA Screening System (Code No. 632636; 50 rxns)
· Guide-it sgRNA In Vitro Transcription Kit (Code No. 632635)
· Guide-it sgRNA Screening Kit (Code No. 632639)

 
 
 
■ 关联资料
点击查看Guide-it sgRNA体外转录和筛选系统宣传页 sgRNA体外转录和筛选
 
 
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