Gelite Safe核酸凝胶染料* 10,000X DMSO溶液*

	货号	17704	存储条件	室温储存不要冷冻, 避免光照
	规格	100 ul	价格	924
	Ex (nm)	513	Em (nm)	552
	分子量	N/A	溶剂	Water
	产品详细介绍

简要概述

Gelite Safe 核酸凝胶染料是一种非常敏感且稳定的荧光染料，可用于电泳凝胶中的DNA检测。 与高毒性的溴化乙锭（EtBr）不同，Gelite Safe经过专门设计，具有较低的危害性，无细胞毒性和诱变性，与EtBr和SYBR®Safe相比，具有更高的灵敏度和更低的背景荧光。 Gelite Safe的独特光谱特性扩展了其仪器兼容性，包括紫外和蓝光透射仪，凝胶记录系统和激光扫描仪。无需繁杂的操作，可在在绿色或红色通道中进行检测。金畔生物是AAT Bioquest的中国代理商，为您提供最优质的Gelite Safe核酸凝胶染料* 10,000X DMSO溶液*。 

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产品说明书

样品实验方案

工作溶液配制

Gelite Safe 工作溶液

在7.5-8.5的pH范围内使用（例如，TAE、TBE或TE pH 8.2）缓冲液稀释10,000X储备试剂来制备1X Gelite Safe工作溶液。
注意：在水中配制的染色溶液比在缓冲液中配制的溶液不稳定，必须在24小时内使用。

后染色方案
1.根据您的标准方案运行凝胶。
2.将凝胶放在合适的聚丙烯容器中。 轻轻加入足量的1X染色溶液以浸没凝胶。
注意：请勿使用玻璃容器，因为它会吸收染色溶液中的许多染料。
3.在室温下避光轻轻搅拌凝胶约30至60分钟。
4.使用300 nm / 254 nm紫外透射仪或使用长距离绿色滤光片（例如SYBR®滤光片，GelStar®滤光片，GelGreen®滤光片或GelRed®滤光片）的凝胶扫描仪对凝胶成像。

预染色方案
1.使用标准方案准备琼脂糖凝胶溶液。
2.在倒入凝胶之前，将10,000X Gelite Safe储备试剂以1：10,000的比例稀释到凝胶溶液中并彻底混合。
3.根据您的标准方案运行凝胶。
4.使用300 nm / 254 nm紫外透射仪或使用长距离绿色滤光片（例如SYBR®滤光片，GelStar®滤光片，GelGreen®滤光片或GelRed®滤光片）的凝胶扫描仪对凝胶成像。

图示

图1.使用Gelite Safe，EtBr和SYBR®Safe在TBE缓冲液中的1％琼脂糖凝胶中检测DNA的比较。 从左到右分别以100 ng，50 ng和25 ng的量加载1 kb DNA的两倍连续稀释液。 根据制造商推荐的浓度，将凝胶用Gelite Safe，EtBr和SYBR®Safe染色60分钟，并使用ChemiDoc 成像系统（Bio-Rad®）进行成像。 使用装有GelGreen和GelRed滤光片的300 nm荧光透射仪对凝胶进行照明。

参考文献

Limited Proteolysis and Gel Electrophoresis in the Presence of Metal Cations: Au(III)-binding Luminescent Domain in Serum Albumins.
Authors: Dixon, Jacob M and Egusa, Shunji
Journal: Journal of visualized experiments : JoVE (2021)

High affinity of AS1411 toward copper; its application in a sensitive aptasensor for copper detection.
Authors: Bahreyni, Amirhossein and Ramezani, Mohammad and Alibolandi, Mona and Hassanzadeh, Pirooz and Abnous, Khalil and Taghdisi, Seyed Mohammad
Journal: Analytical biochemistry (2019): 1-9

The efficacy and tolerability of 5-aminolevulinic acid 5% thermosetting gel photodynamic therapy (PDT) in the treatment of mild-to-moderate acne vulgaris. A two-center, prospective assessor-blinded, proof-of-concept study.
Authors: Serini, Stefano Maria and Cannizzaro, Maria Vittoria and Dattola, Annunziata and Garofalo, Virginia and Del Duca, Esther and Ventura, Alessandra and Milani, Massimo and Campione, Elena and Bianchi, Luca
Journal: Journal of cosmetic dermatology (2019): 156-162

Transfection Studies with Colloidal Systems Containing Highly Purified Bipolar Tetraether Lipids from Sulfolobus acidocaldarius.
Authors: Engelhardt, Konrad H and Pinnapireddy, Shashank Reddy and Baghdan, Elias and Jedelská, Jarmila and Bakowsky, Udo
Journal: Archaea (Vancouver, B.C.) (2017): 8047149

Metallo-supramolecular gels based on a multitopic cyclam bis-terpyridine platform.
Authors: Gasnier, Aurélien and Royal, Guy and Terech, Pierre
Journal: Langmuir : the ACS journal of surfaces and colloids (2009): 8751-62

Characterization of hepatic L-threonine dehydrogenase of chicken.
Authors: Yuan, J H and Austic, R E
Journal: Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology (2001): 65-73

[Cloning of group A streptococcal pyrogenic exotoxin-B gene and its recombinant protein expression in culture supernatant].
Authors: Watanabe, Y
Journal: Journal of Nippon Medical School = Nippon Ika Daigaku zasshi (2001): 222-32

Purification and characterization of chondroitin 4-sulfotransferase from the culture medium of a rat chondrosarcoma cell line.
Authors: Yamauchi, S and Hirahara, Y and Usui, H and Takeda, Y and Hoshino, M and Fukuta, M and Kimura, J H and Habuchi, O
Journal: The Journal of biological chemistry (1999): 2456-63

Comparison of tube and gel red blood cell agglutination techniques in detecting chimeras after major ABO-mismatched allogeneic hematopoietic stem cell transplantation.
Authors: Kupferman, M J and Cipolone, K M and Procter, J L and Stroncek, D F
Journal: Immunohematology (1998): 63-7

Expression of pig heart mitochondrial NADP-dependent isocitrate dehydrogenase in Escherichia coli.
Authors: Soundar, S and Jennings, G T and McAlister-Henn, L and Colman, R F
Journal: Protein expression and purification (1996): 305-12

Fura-10，AM

	货号	21114	存储条件	在零下15度以下保存, 避免光照
	规格	5×50 ug	价格	1200
	Ex (nm)	354	Em (nm)	524
	分子量	1194.14	溶剂	DMSO
	产品详细介绍

简要概述

产品基本信息

货号：21114

产品名称：Fura-10，AM

规格：5×50 ug

储存条件：-15℃避光防潮

保质期：12个月

产品物理化学光谱特性

分子量：1194.14

溶剂：DMSO

产品介绍

在比例钙离子指示剂中，Fura-2和Indo-1是两个最受欢迎的指示剂。 但是，使用这两种钙离子指示剂仍然存在一些挑战，特别是对于活细胞。 Fura-2的紫外线激发导致快速的光致漂白。 几年前推出了Fura-8 ，将激发光移向可见光。 尽管Fura-8表现出信号/背景比率的明显改善，但它不能像Fura-2那样很好地保留在活细胞中。 Fura-10最近被引入以解决该细胞保留问题。 在没有丙磺舒的情况下，Fura 10表现出信号/背景比的明显改善。金畔生物是AAT Bioquest的中国代理商，为您提供最优质的Fura-10，AM。 

参考文献

Acupotomy Alleviates Energy Crisis at Rat Myofascial Trigger Points.
Authors: Zhang, Yi and Du, Ning-Yu and Chen, Chen and Wang, Tong and Wang, Li-Juan and Shi, Xiao-Lu and Li, Shu-Ming and Guo, Chang-Qing
Journal: Evidence-based complementary and alternative medicine : eCAM (2020): 5129562

High-salt intake increases TRPC3 expression and enhances TRPC3-mediated calcium influx and systolic blood pressure in hypertensive patients.
Authors: Hu, Yingru and Xia, Weijie and Li, Yingsha and Wang, Qianran and Lin, Shaoyang and Wang, Bin and Zhou, Cui and Cui, Yuanting and Jiang, Yanli and Pu, Xiaona and Wei, Xiao and Wu, Hao and Zhang, Hengshu and Zhu, Zhiming and Liu, Daoyan and Li, Zhiyong
Journal: Hypertension research : official journal of the Japanese Society of Hypertension (2020)

Histamine induces intracellular Ca2+ oscillations and nitric oxide release in endothelial cells from brain microvascular circulation.
Authors: Berra-Romani, Roberto and Faris, Pawan and Pellavio, Giorgia and Orgiu, Matteo and Negri, Sharon and Forcaia, Greta and Var-Gaz-Guadarrama, Verónica and Garcia-Carrasco, Mario and Botta, Laura and Sancini, Giulio and Laforenza, Umberto and Moccia, Francesco
Journal: Journal of cellular physiology (2020): 1515-1530

Pharmacological and genetic characterisation of the canine P2X4 receptor.
Authors: Sophocleous, Reece A and Berg, Tracey and Finol-Urdaneta, Rocio K and Sluyter, Vanessa and Keshiya, Shikara and Bell, Lachlan and Curtis, Stephen J and Curtis, Belinda L and Seavers, Aine and Bartlett, Rachael and Dowton, Mark and Stokes, Leanne and Ooi, Lezanne and Sluyter, Ronald
Journal: British journal of pharmacology (2020)

Reduced store-operated Ca2+ entry impairs mesenteric artery function in response to high external glucose in type 2 diabetic ZDF rats.
Authors: Schach, Christian and Wester, Michael and Leibl, Florian and Redel, Andreas and Gruber, Michael and Maier, Lars S and Endemann, Dierk and Wagner, Stefan
Journal: Clinical and experimental pharmacology & physiology (2020)

Rumex acetosa modulates platelet function and inhibits thrombus formation in rats.
Authors: Jeong, Dahye and Irfan, Muhammad and Lee, Dong-Ha and Hong, Seung-Bok and Oh, Jae-Wook and Rhee, Man Hee
Journal: BMC complementary medicine and therapies (2020): 98

A Na+ /Ca2+ exchanger of the olive pathogen Pseudomonas savastanoi pv. savastanoi is critical for its virulence.
Authors: Moretti, Chiaraluce and Trabalza, Simone and Granieri, Letizia and Caballo-Ponce, Eloy and Devescovi, Giulia and Del Pino, Alberto Marco and Ramos, Cayo and Venturi, Vittorio and van den Burg, Harrold A and Buonaurio, Roberto and Palmerini, Carlo Alberto
Journal: Molecular plant pathology (2019): 716-730

A3 receptor agonist, Cl-IBMECA, potentiate glucose-induced insulin secretion from MIN6 insulinoma cells possibly through transient Ca2+ entry.
Authors: Keyvanloo Shahrestanaki, Mohammad and Aghaei, Mahmoud
Journal: Research in pharmaceutical sciences (2019): 107-114

Accumulation of intramyocyte TRPV1-mediated calcium during heat stress is inhibited by concomitant muscle contractions.
Authors: Ikegami, Ryo and Eshima, Hiroaki and Mashio, Takuro and Ishiguro, Tomosada and Hoshino, Daisuke and Poole, David C and Kano, Yutaka
Journal: Journal of applied physiology (Bethesda, Md. : 1985) (2019): 691-698

Acidification is an Essential Process of Cold Atmospheric Plasma and Promotes the Anti-Cancer Effect on Malignant Melanoma Cells.
Authors: Schneider, Christin and Gebhardt, Lisa and Arndt, Stephanie and Karrer, Sigrid and Zimmermann, Julia L and Fischer, Michael J M and Bosserhoff, Anja-Katrin
Journal: Cancers (2019)

Texas 红-X，酸 CAS 199745-67-0

	货号	469	存储条件	在零下15度以下保存, 避免光照
	规格	10 mg	价格	2388
	Ex (nm)	595	Em (nm)	613
	分子量	719.87	溶剂	DMF
	产品详细介绍

简要概述

产品基本信息

货号：469

产品名称：Texas 红-X，酸

CAS：199745-67-0

规格：10mg

储存条件：-15℃，避光干燥

保质期：12个月

物理化学光谱特性

分子量：719.87

溶剂：DMF

Ex(nm):595

Em(nm):613

产品介绍

Texas Red-X,酸是美国AAT Bioquest生产的荧光染料，与TexasRed®相比，Texas Red-X染料在荧光团和羧基之间包含一个额外的七原子氨基己酰基间隔基（“X”）。该间隔物有助于将荧光团与其连接点分开，从而潜在地减少了荧光团与其结合的生物分子之间的相互作用。该产品是单一的5个异构体，可提供更可重复的结果。它具有与德州红（Texas Red）相同的光谱特性，可用于构建最大激发/发射〜595/615 nm的亮红色荧光生物共轭物（TexasRed®是ThermoFisher的商标）。金畔生物是AAT Bioquest的中国代理商，为您提供最优质的Texas Red-X,酸。 

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参考文献

Blood-brain barrier permeability towards small and large tracers in a mouse model of osmotic demyelination syndrome.
Authors: Scalisi, Joshua and Balau, Benoît and Deneyer, Lynn and Bouchat, Joanna and Gilloteaux, Jacques and Nicaise, Charles
Journal: Neuroscience letters (2021): 135665

Detection of Tumor-Specific PTPmu in Gynecological Cancer and Patient Derived Xenografts.
Authors: Vincent, Jason and Craig, Sonya E L and Johansen, Mette L and Narla, Jyosthna and Avril, Stefanie and DiFeo, Analisa and Brady-Kalnay, Susann M
Journal: Diagnostics (Basel, Switzerland) (2021)

Interactions of different lipoproteins with supported phospholipid raft membrane (SPRM) patterns to understand similar in-vivo processes.
Authors: Sapuri-Butti, Annapoorna R and Wang, Limin and Tetali, Sarada D and Rutledge, John C and Parikh, Atul N
Journal: Biochimica et biophysica acta. Biomembranes (2021): 183535

Novel aptasensor-based assay of sonic hedgehog ligand for detection of portal vein invasion of hepatocellular carcinoma.
Authors: Li, Hui-Yan and Yin, Fang-Fei and Li, Xin-Yi and Jia, Wan-Nan and Ding, Jia and Zhang, Li and Wang, Zhong-Hua and Hu, Qin-Qin and Zuo, Jie-Liang and Jia, Hu-Liang and Wang, Li-Hua and Fan, Chun-Hai and Gu, Hong-Zhou and Wu, Jian
Journal: Biosensors & bioelectronics (2021): 112738

Quirks of dye nomenclature. 16. Dyes, and a pigment, named after places.
Authors: Cooksey, C J
Journal: Biotechnic & histochemistry : official publication of the Biological Stain Commission (2021): 1-15

Visualization and quantification of pancreatic tumor stroma in fresh tissue via ultraviolet surface excitation.
Authors: Vincent, Phuong and Bruza, Petr and Palisoul, Scott M and Gunn, Jason R and Samkoe, Kimberley S and Hoopes, P Jack and Hasan, Tayyaba and Pogue, Brian W
Journal: Journal of biomedical optics (2021)

A multifunctional DNA nanostructure based on multicolor FRET for nuclease activity assay.
Authors: Hu, Juan and Li, Wen-Can and Qiu, Jian-Ge and Jiang, BingHua and Zhang, Chun-Yang
Journal: The Analyst (2020): 6054-6060

A new aminoglycoside etimicin shows low nephrotoxicity and ototoxicity in zebrafish embryos.
Authors: Shao, Weihao and Zhong, Dan and Jiang, Haowei and Han, Yujie and Yin, Yu and Li, Ruining and Qian, Xiuping and Chen, Daijie and Jing, Lili
Journal: Journal of applied toxicology : JAT (2020)

Acoustic Poration and Dynamic Healing of Mammalian Cell Membranes during Inkjet Printing.
Authors: Barui, Srimanta and Saunders, Rachel E and Naskar, Sharmistha and Basu, Bikramjit and Derby, Brian
Journal: ACS biomaterials science & engineering (2020): 749-757

Characterization of the Blood-Brain Barrier Integrity and the Brain Transport of SN-38 in an Orthotopic Xenograft Rat Model of Diffuse Intrinsic Pontine Glioma.
Authors: Chaves, Catarina and Declèves, Xavier and Taghi, Meryam and Menet, Marie-Claude and Lacombe, Joelle and Varlet, Pascale and Olaciregui, Nagore G and Carcaboso, Angel M and Cisternino, Salvatore
Journal: Pharmaceutics (2020)

相关产品 

一氧化氮NO荧光探针DAF-FM CAS 254109-20-1

	货号	16296	存储条件	在零下15度以下保存, 避免光照
	规格	1 mg	价格	3732
	Ex (nm)		Em (nm)
	分子量	412.35	溶剂	DMSO
	产品详细介绍

简要概述

产品基本信息

货号：16296

产品名称：一氧化氮NO荧光探针DAF-FM

CAS：254109-20-1

规格：1mg

储存条件：-15℃避光防潮

保质期：12个月

产品物理化学光谱特性

分子量：412.35

溶剂：DMSO

产品介绍

DAF-2被广泛用于检测细胞中的一氧化氮（NO）。DAF-FM是DAF的改进版本。与DAF-2相比，DAF-FM是用于检测NO的更加灵敏且光稳定性更高的荧光探针。此外，DAF-FM对pH的敏感性比DAF-2低得多。DAF-FM不能像双乙酸酯衍生物DAF-DA那样具有细胞渗透性，但是它不需要被胞质酶激活。金畔生物是AAT Bioquest的中国代理商，为您提供最优质的一氧化氮NO荧光探针DAF-FM。 

参考文献

Association between expression of AMPK pathway and adiponectin, leptin, and vascular endothelial function in rats with coronary heart disease.
Authors: Li, J-M and Lu, W and Ye, J and Han, Y and Chen, H and Wang, L-S
Journal: European review for medical and pharmacological sciences (2020): 905-914

Histamine induces intracellular Ca2+ oscillations and nitric oxide release in endothelial cells from brain microvascular circulation.
Authors: Berra-Romani, Roberto and Faris, Pawan and Pellavio, Giorgia and Orgiu, Matteo and Negri, Sharon and Forcaia, Greta and Var-Gaz-Guadarrama, Verónica and Garcia-Carrasco, Mario and Botta, Laura and Sancini, Giulio and Laforenza, Umberto and Moccia, Francesco
Journal: Journal of cellular physiology (2020): 1515-1530

Methods for Measuring Nitrate Reductase, Nitrite Levels, and Nitric Oxide from Plant Tissues.
Authors: Wany, Aakanksha and Pathak, Pradeep Kumar and Gupta, Kapuganti Jagadis
Journal: Methods in molecular biology (Clifton, N.J.) (2020): 15-26

Nitric oxide restores peripheral blood mononuclear cell adhesion against hypoxia via NO-cGMP signalling.
Authors: Behera, Jyotirmaya and Nagarajan, Shunmugam and Saran, Uttara and Kumar, Ravi and Keshri, Gaurav K and Suryakumar, Geetha and Chatterjee, Suvro
Journal: Cell biochemistry and function (2020)

Nitro-Oleic Acid in Seeds and Differently Developed Seedlings of Brassica napus L.
Authors: Vollár, Martin and Feigl, Gábor and Oláh, Dóra and Horváth, Attila and Molnár, Árpád and Kúsz, Norbert and Ördög, Attila and Csupor, Dezső and Kolbert, Zsuzsanna
Journal: Plants (Basel, Switzerland) (2020)

Omentin-1 protects against high glucose-induced endothelial dysfunction via the AMPK/PPARδ signaling pathway.
Authors: Liu, Fang and Fang, Shaohong and Liu, Xinxin and Li, Ji and Wang, Xuedong and Cui, Jinjin and Chen, Tao and Li, Zhaoying and Yang, Fan and Tian, Jiangtian and Li, Hulun and Yin, Li and Yu, Bo
Journal: Biochemical pharmacology (2020): 113830

7-Keto-Cholesterol and Cholestan-3beta, 5alpha, 6beta-Triol Induce Eryptosis through Distinct Pathways Leading to NADPH Oxidase and Nitric Oxide Synthase Activation.
Authors: Attanzio, Alessandro and Frazzitta, Anna and Cilla, Antonio and Livrea, Maria A and Tesoriere, Luisa and Allegra, Mario
Journal: Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology (2019): 933-947

A reliable fluorimetric method to screen the nitric oxide synthase inhibitors in 96 well plate.
Authors: Agrawal, Saurabh and Kumari, Rita and Luthra, Pratibha Mehta
Journal: Analytical biochemistry (2019): 42-44

Acute spatial spread of NO-mediated potentiation during hindpaw ischaemia in mice.
Authors: Onishi, Takeshi and Watanabe, Tatsunori and Sasaki, Mika and Kamiya, Yoshinori and Horie, Masao and Tsukano, Hiroaki and Hishida, Ryuichi and Kohno, Tatsuro and Takebayashi, Hirohide and Baba, Hiroshi and Shibuki, Katsuei
Journal: The Journal of physiology (2019): 3441-3455

Androgens drive microvascular endothelial dysfunction in women with polycystic ovary syndrome: role of the endothelin B receptor.
Authors: Usselman, Charlotte W and Yarovinsky, Timur O and Steele, Frances E and Leone, Cheryl A and Taylor, Hugh S and Bender, Jeffrey R and Stachenfeld, Nina S
Journal: The Journal of physiology (2019): 2853-2865

iFluor 665马来酰亚胺

简要概述

产品基本信息

货号：1554

产品名称：iFluor 665马来酰亚胺

规格：1mg

储存条件：-15℃，避光干燥

保质期：12个月

物理化学光谱特性

分子量：1012.08

溶剂：DMSO

Ex(nm):667

Em(nm):692

Abs（nm）:661

产品介绍

AAT Bioquest的iFluor 染料针对标记蛋白质（特别是抗体）进行了优化。这些染料是明亮的，耐光的，并且对蛋白质的淬灭作用极小。它们可以被荧光仪器的主要激光线（例如350、405、488、532、555、633和647 nm）激发。 iFluor 665系列的光谱特性类似于AlexaFluor®660（AlexaFluor®是Invitrogen的商标）。 iFluor 665系列的荧光在pH值从3到11时不受pH值的影响。这些光谱特性使该新染料系列成为AlexaFluor®660的出色替代品。在相同条件下，iFluor 665在某些抗体上可提供更强的荧光信号。 iFluor 665马来酰亚胺相当稳定，即使在中性或弱酸性条件下，对蛋白质硫醇基也表现出良好的反应性和选择性。

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参考文献

Recognition of Invasive Prostate Cancer Using a GHRL Polypeptide Probe Targeting GHSR in a Mouse Model In Vivo.
Authors: Ye, Huamao and Yang, Yue and Chen, Rui and Shi, Xiaolei and Fang, Yu and Yang, Jun and Dong, Yuanzhen and Chen, Lili and Xia, Jianghua and Wang, Chao and Yang, Chenghua and Feng, Jun and Wang, Yang and Feng, Xiang and Lü, Chen
Journal: Current pharmaceutical design (2020): 1614-1621

A rapid sensitive, flow cytometry-based method for the detection of Plasmodium vivax-infected blood cells.
Authors: Roobsoong, Wanlapa and Maher, Steven P and Rachaphaew, Nattawan and Barnes, Samantha J and Williamson, Kim C and Sattabongkot, Jetsumon and Adams, John H
Journal: Malaria journal (2014): 55

Reengineering the optical absorption cross-section of photosynthetic reaction centers.
Authors: Dutta, Palash K and Lin, Su and Loskutov, Andrey and Levenberg, Symon and Jun, Daniel and Saer, Rafael and Beatty, J Thomas and Liu, Yan and Yan, Hao and Woodbury, Neal W
Journal: Journal of the American Chemical Society (2014): 4599-604

Comparison of a chimeric anti-carcinoembryonic antigen antibody conjugated with visible or near-infrared fluorescent dyes for imaging pancreatic cancer in orthotopic nude mouse models.
Authors: Maawy, Ali A and Hiroshima, Yukihiko and Kaushal, Sharmeela and Luiken, George A and Hoffman, Robert M and Bouvet, Michael
Journal: Journal of biomedical optics (2013): 126016

Nucleic acid sandwich hybridization assay with quantum dot-induced fluorescence resonance energy transfer for pathogen detection.
Authors: Chou, Cheng-Chung and Huang, Yi-Han
Journal: Sensors (Basel, Switzerland) (2012): 16660-72

Single-molecule dynamics of phytochrome-bound fluorophores probed by fluorescence correlation spectroscopy.
Authors: Miller, Abigail E and Fischer, Amanda J and Laurence, Ted and Hollars, Christopher W and Saykally, Richard J and Lagarias, J Clark and Huser, Thomas
Journal: Proceedings of the National Academy of Sciences of the United States of America (2006): 11136-41

A far-red fluorescent contrast agent to image epidermal growth factor receptor expression.
Authors: Hsu, Elizabeth R and Anslyn, Eric V and Dharmawardhane, Su and Alizadeh-Naderi, Reza and Aaron, Jesse S and Sokolov, Konstantin V and El-Naggar, Adel K and Gillenwater, Ann M and Richards-Kortum, Rebecca R
Journal: Photochemistry and photobiology (2004): 272-9

小麦胚芽凝集素，AF488标记

简要概述

产品基本信息

货号：25500

产品名称：小麦胚芽凝集素，AF488标记

规格：1mg

储存条件：-15℃避光防潮

保质期：12个月

产品物理化学光谱特性

Ex(nm)：499

Em(nm)：520

量子产率(cm ^-1 M ^-1)：73000

消光系数：0.92

产品介绍

小麦胚芽凝集素（WGA）是一种凝集素，可与N-乙酰基-D-葡萄糖胺和唾液酸结合。它是生物学研究最多且最有用的凝集素。由于WGA与糖结合物结合，其衍生物和结合物被广泛用于标记细胞膜和纤维化疤痕组织，以进行荧光成像和分析。WGA的碳水化合物结合特异性针对β-1,4-GlcNAc连接的残基，即壳聚糖。每个单体包含两个相同的，非相互作用的结合位点，它们与3或4个β-1,4-GlcNAc单元互补。在检查的单糖中，只有GlcNAc与WGA结合。ManNAc不结合，而GalNAc仅弱结合。WGA的AF488共轭物相当于WGA的AlexaFluor®488共轭物。AF488 WGA共轭物与唾液酸和N-乙酰氨基葡萄糖残基结合后表现出明亮的荧光（AlexaFluor®是ThermoFisher的商标）。金畔生物是AAT Bioquest的中国代理商，为您提供最优质的小麦胚芽凝集素，AF488标记。 

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参考文献

Membrane-associated gamma-glutamyl transferase and alkaline phosphatase in the context of concanavalin A- and wheat germ agglutinin-reactive glycans mark seminal prostasome populations from normozoospermic and oligozoospermic men.
Authors: Janković, Tamara and Goč, Sanja and Mitić, Ninoslav and Danilović Luković, Jelena and Janković, Miroslava
Journal: Upsala journal of medical sciences (2020): 10-18

Succinylated Wheat Germ Agglutinin Colocalizes with the Toxoplasma gondii Cyst Wall Glycoprotein CST1.
Authors: Guevara, Rebekah B and Fox, Barbara A and Bzik, David J
Journal: mSphere (2020)

Wheat germ agglutinin is a biomarker of whole grain content in wheat flour and pasta.
Authors: Killilea, David W and McQueen, Rebecca and Abegania, Judi R
Journal: Journal of food science (2020): 808-815

Wheat germ agglutinin is involved in the protective action of 24-epibrassinolide on the roots of wheat seedlings under drought conditions.
Authors: Avalbaev, Azamat and Bezrukova, Marina and Allagulova, Chulpan and Lubyanova, Alsu and Kudoyarova, Guzel and Fedorova, Kristina and Maslennikova, Dilara and Yuldashev, Ruslan and Shakirova, Farida
Journal: Plant physiology and biochemistry : PPB (2020): 420-427

Wheat germ agglutinin liposomes with surface grafted cyclodextrins as bioadhesive dual-drug delivery nanocarriers to treat oral cells.
Authors: Wijetunge, Sashini S and Wen, Jianchuan and Yeh, Chih-Ko and Sun, Yuyu
Journal: Colloids and surfaces. B, Biointerfaces (2020): 110572

Wheat germ agglutinin-conjugated fluorescent pH sensors for visualizing proton fluxes.
Authors: Zhang, Lejie and Zhang, Mei and Bellve, Karl and Fogarty, Kevin E and Castro, Maite A and Brauchi, Sebastian and Kobertz, William R
Journal: The Journal of general physiology (2020)

Bacterial expression, purification and biophysical characterization of wheat germ agglutinin and its four hevein-like domains.
Authors: Leyva, Eduardo and Medrano-Cerano, Jorge L and Cano-Sánchez, Patricia and López-González, Itzel and Gómez-Velasco, Homero and Del Río-Portilla, Federico and García-Hernández, Enrique
Journal: Biopolymers (2019): e23242

Enhanced anti-colon cancer efficacy of 5-fluorouracil by epigallocatechin-3- gallate co-loaded in wheat germ agglutinin-conjugated nanoparticles.
Authors: Wang, Ruoning and Huang, Jinyu and Chen, Jian and Yang, Mengmeng and Wang, Honglan and Qiao, Hongzhi and Chen, Zhipeng and Hu, Lihong and Di, Liuqing and Li, Junsong
Journal: Nanomedicine : nanotechnology, biology, and medicine (2019): 102068

Targeted delivery of etoposide, carmustine and doxorubicin to human glioblastoma cells using methoxy poly(ethylene glycol)‑poly(ε‑caprolactone) nanoparticles conjugated with wheat germ agglutinin and folic acid.
Authors: Kuo, Yung-Chih and Chang, Yu-Hsuan and Rajesh, Rajendiran
Journal: Materials science & engineering. C, Materials for biological applications (2019): 114-128

Towards the preparation of synthetic outer membrane vesicle models with micromolar affinity to wheat germ agglutinin using a dialkyl thioglycoside.
Authors: Fayolle, Dimitri and Berthet, Nathalie and Doumeche, Bastien and Renaudet, Olivier and Strazewski, Peter and Fiore, Michele
Journal: Beilstein journal of organic chemistry (2019): 937-946

DNP-PEG4酸 CAS 858126-76-8

	货号	2022	存储条件	在零下15度以下保存, 避免光照
	规格	25 mg	价格	1164
	Ex (nm)		Em (nm)
	分子量	431.40	溶剂	DMSO
	产品详细介绍

简要概述

产品基本信息

货号：2022

产品名称：DNP-PEG4酸

CAS：858126-76-8

规格：25mg

储存条件：-15℃，避光干燥

保质期：12个月

物理化学光谱特性

分子量：431.40

溶剂：DMSO

产品介绍

与常用的DNP-X酸（＃2020）相比，DNP-PEG4酸具有更好的水溶性。它是可被抗DNP抗体识别的探针的胺反应性构建基块。它也可以用作与Trp或Tyr配对的出色的胺反应性FRET淬灭剂。在EDC和DCC存在下，其末端羧基容易与伯胺反应形成稳定的酰胺键。亲水性PEG接头增加了化合物在水性介质中的水溶性。

参考文献

Nano-Snowflower of Gold Nanoparticles-Ruthenium Metallopolymer-Carbon Nanotubes Binding Anti-DNP IgE Antibody.
Authors: Li, Huayang and Wu, Jie and Melnyczuk, John M and Olubi, Omotunde and Lewis, Lauchon I and Cao, Yang and Nagappan, Peri and Khan, Shafiq A and Ingram, Conrad W and Harruna, Issifu I
Journal: Journal of nanoscience and nanotechnology (2015): 5733-40

Peptide mimics of hapten DNP: the effect of affinity of anti-DNP monoclonal antibodies for the selection of phage-displayed mimotopes.
Authors: Kalaycioglu, Atila T and Russell, Peter H and Howard, Colin R
Journal: Journal of immunological methods (2011): 36-42

Generation of heterogeneous rabbit anti-DNP antibodies by gene conversion and hypermutation of rearranged VL and VH genes during clonal expansion of B cells in splenic germinal centers.
Authors: Sehgal, D and Schiaffella, E and Anderson, A O and Mage, R G
Journal: European journal of immunology (2000): 3634-44

Determination of the binding of ligands containing the N-2,4-dinitrophenyl group to bivalent monoclonal rat anti-DNP antibody using affinity capillary electrophoresis.
Authors: Mammen, M and Gomez, F A and Whitesides, G M
Journal: Analytical chemistry (1995): 3526-35

Fast solid support detection of human papillomavirus in in vitro amplified DNA using a DNP-anti DNP monoclonal antibody couple.
Authors: Baccard-Longère, M and Alpha-Bazin, B and Chypre, C and Sauvaigo, S and Téoule, R and Bernard, P and Seigneurin, J M
Journal: Journal of virological methods (1994): 29-38

[Effects of Celosia argentea and Cucurbita moschata extracts on anti-DNP IgE antibody production in mice].
Authors: Imaoka, K and Ushijima, H and Inouye, S and Takahashi, T and Kojima, Y
Journal: Arerugi = [Allergy] (1994): 652-9

[Effects of Perilla frutescens extract on anti-DNP IgE antibody production in mice].
Authors: Imaoka, K and Inouye, S and Takahashi, T and Kojima, Y
Journal: Arerugi = [Allergy] (1993): 74-80

Effect of anti-DNP IgG1- and IgG2a-secreting hybridomas in vivo on the development of an anti-DNP IgE antibody response in mice.
Authors: Hagen, M and Morrison, B and Robbinson, D and Strejan, G H
Journal: International archives of allergy and immunology (1992): 146-53

Mechanism of allergic cross-reactions–I. Multispecific binding of ligands to a mouse monoclonal anti-DNP IgE antibody.
Authors: Varga, J M and Kalchschmid, G and Klein, G F and Fritsch, P
Journal: Molecular immunology (1991): 641-54

Mechanism of allergic cross-reactions–II. Cross-stimulation, by chemically unrelated ligands, of rat basophilic leukemia cells sensitized with an anti-DNP IgE antibody.
Authors: Varga, J M and Kalchschmid, G and Klein, G F and Fritsch, P
Journal: Molecular immunology (1991): 655-9

小麦胚芽凝集素，AF594标记

简要概述

产品基本信息

货号：25509

产品名称：小麦胚芽凝集素，AF594标记

规格：1mg

储存条件：-15℃避光防潮

保质期：12个月

产品物理化学光谱特性

Ex(nm)：590

Em(nm)：618

量子产率(cm ^-1 M ^-1)：92000

消光系数：0.66

产品介绍

小麦胚芽凝集素（WGA）是一种凝集素，可与N-乙酰基-D-葡萄糖胺和唾液酸结合。由于WGA与糖结合物结合，其衍生物和结合物被广泛用于标记细胞膜和纤维化疤痕组织，以进行荧光成像和分析。WGA的AF594缀合物等同于WGA的594祝贺我。AF594 WGA共轭物与唾液酸和N-乙酰氨基葡萄糖残基结合后具有AlexaFluor®594染料的明亮红色荧光（AlexaFluor®是ThermoFisher的商标）。金畔生物是AAT Bioquest的中国代理商，为您提供最优质的小麦胚芽凝集素，AF594标记。 

点击查看光谱

参考文献

Membrane-associated gamma-glutamyl transferase and alkaline phosphatase in the context of concanavalin A- and wheat germ agglutinin-reactive glycans mark seminal prostasome populations from normozoospermic and oligozoospermic men.
Authors: Janković, Tamara and Goč, Sanja and Mitić, Ninoslav and Danilović Luković, Jelena and Janković, Miroslava
Journal: Upsala journal of medical sciences (2020): 10-18

Succinylated Wheat Germ Agglutinin Colocalizes with the Toxoplasma gondii Cyst Wall Glycoprotein CST1.
Authors: Guevara, Rebekah B and Fox, Barbara A and Bzik, David J
Journal: mSphere (2020)

Wheat germ agglutinin is a biomarker of whole grain content in wheat flour and pasta.
Authors: Killilea, David W and McQueen, Rebecca and Abegania, Judi R
Journal: Journal of food science (2020): 808-815

Wheat germ agglutinin is involved in the protective action of 24-epibrassinolide on the roots of wheat seedlings under drought conditions.
Authors: Avalbaev, Azamat and Bezrukova, Marina and Allagulova, Chulpan and Lubyanova, Alsu and Kudoyarova, Guzel and Fedorova, Kristina and Maslennikova, Dilara and Yuldashev, Ruslan and Shakirova, Farida
Journal: Plant physiology and biochemistry : PPB (2020): 420-427

Wheat germ agglutinin liposomes with surface grafted cyclodextrins as bioadhesive dual-drug delivery nanocarriers to treat oral cells.
Authors: Wijetunge, Sashini S and Wen, Jianchuan and Yeh, Chih-Ko and Sun, Yuyu
Journal: Colloids and surfaces. B, Biointerfaces (2020): 110572

Wheat germ agglutinin-conjugated fluorescent pH sensors for visualizing proton fluxes.
Authors: Zhang, Lejie and Zhang, Mei and Bellve, Karl and Fogarty, Kevin E and Castro, Maite A and Brauchi, Sebastian and Kobertz, William R
Journal: The Journal of general physiology (2020)

Bacterial expression, purification and biophysical characterization of wheat germ agglutinin and its four hevein-like domains.
Authors: Leyva, Eduardo and Medrano-Cerano, Jorge L and Cano-Sánchez, Patricia and López-González, Itzel and Gómez-Velasco, Homero and Del Río-Portilla, Federico and García-Hernández, Enrique
Journal: Biopolymers (2019): e23242

Enhanced anti-colon cancer efficacy of 5-fluorouracil by epigallocatechin-3- gallate co-loaded in wheat germ agglutinin-conjugated nanoparticles.
Authors: Wang, Ruoning and Huang, Jinyu and Chen, Jian and Yang, Mengmeng and Wang, Honglan and Qiao, Hongzhi and Chen, Zhipeng and Hu, Lihong and Di, Liuqing and Li, Junsong
Journal: Nanomedicine : nanotechnology, biology, and medicine (2019): 102068

Targeted delivery of etoposide, carmustine and doxorubicin to human glioblastoma cells using methoxy poly(ethylene glycol)‑poly(ε‑caprolactone) nanoparticles conjugated with wheat germ agglutinin and folic acid.
Authors: Kuo, Yung-Chih and Chang, Yu-Hsuan and Rajesh, Rajendiran
Journal: Materials science & engineering. C, Materials for biological applications (2019): 114-128

Towards the preparation of synthetic outer membrane vesicle models with micromolar affinity to wheat germ agglutinin using a dialkyl thioglycoside.
Authors: Fayolle, Dimitri and Berthet, Nathalie and Doumeche, Bastien and Renaudet, Olivier and Strazewski, Peter and Fiore, Michele
Journal: Beilstein journal of organic chemistry (2019): 937-946

6-ROXtra,SE 优于6-ROX

	货号	401	存储条件	在零下15度以下保存, 避免光照
	规格	1 mg	价格	1164
	Ex (nm)	578	Em (nm)	595
	分子量	1030.23	溶剂	DMSO
	产品详细介绍

简要概述

产品基本信息

货号：401

产品名称：6-ROXtra,SE 优于6-ROX

规格：1mg

储存条件：-15℃避光防潮

保质期：12个月

产品物理化学光谱特性

分子量：1030.23

溶剂：DMSO

激发波长（nm）：578

发射波长（nm）：595

产品介绍

6-ROXtra,SE是美国AAT Bioquest生产的ROX系列染料。尽管ROX染料具有很强的荧光性，但它极不稳定，一些市售试剂保质期只有几个月。与6-ROX标记化合物相比，我们的6-ROXtra 染料具有更高的稳定性和相同的光谱特性。此外，我们的6-ROXtra 染料比6-ROX染料水溶性更高。我们的初步研究表明，在大多数情况下，6-ROXtra衍生的寡核苷酸比相应的6-ROX寡核苷酸更容易纯化。当与蛋白质偶联时，6-ROX的荧光会被严重淬灭，而6-ROXtra 仍表现出强荧光。金畔生物是AAT Bioquest的中国代理商，为您提供最优质的6-ROXtra,SE。 

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参考文献

Four-color DNA sequencing by synthesis on a chip using photocleavable fluorescent nucleotides
Authors: Seo TS, Bai X, Kim DH, Meng Q, Shi S, Ruparel H, Li Z, Turro NJ, Ju J.
Journal: Proc Natl Acad Sci U S A (2005): 5926

Adsorption of oligonucleotides on PMMA/PNIPAM core-shell latexes: polarity of the PNIPAM shell probed by fluorescence
Authors: Prazeres TJ, Santos AM, Martinho JM, Elaissari A, Pichot C.
Journal: Langmuir (2004): 6834

Evaluation of surface-enhanced resonance Raman scattering for quantitative DNA analysis
Authors: Faulds K, Smith WE, Graham D.
Journal: Anal Chem (2004): 412

Photocleavable fluorescent nucleotides for DNA sequencing on a chip constructed by site-specific coupling chemistry
Authors: Seo TS, Bai X, Ruparel H, Li Z, Turro NJ, Ju J.
Journal: Proc Natl Acad Sci U S A (2004): 5488

Blue light-induced generation of reactive oxygen species in photoreceptor ellipsoids requires mitochondrial electron transport
Authors: Yang JH, Basinger SF, Gross RL, Wu SM.
Journal: Invest Ophthalmol Vis Sci (2003): 1312

HLA-DRB fluorotyping by dark quenching and automated analysis
Authors: Slateva K, Elsner HA, Albis-Camps M, Blasczyk R.
Journal: Tissue Antigens (2001): 250

Influence of fluorophor dye labels on the migration behavior of polymerase chain reaction–amplified short tandem repeats during denaturing capillary electrophoresis
Authors: Hahn M, Wilhelm J, Pingoud A.
Journal: Electrophoresis (2001): 2691

Design, synthesis, and spectroscopic properties of peptide-bridged fluorescence energy-transfer cassettes
Authors: Li Y, Glazer AN.
Journal: Bioconjug Chem (1999): 241

Comparison of fluorescence energy transfer primers with different donor-acceptor dye combinations
Authors: Hung SC, Mathies RA, Glazer AN.
Journal: Anal Biochem (1998): 32

Differential display with carboxy-X-rhodamine-labeled primers and the selection of differentially amplified cDNA fragments without cloning
Authors: Yoshikawa Y, Mukai H, Asada K, Hino F, Kato I.
Journal: Anal Biochem (1998): 82

iFluor 594小麦胚芽凝集素（WGA）缀合物

	货号	25550	存储条件	在零下15度以下保存, 避免光照
	规格	1 mg	价格	1200
	Ex (nm)	588	Em (nm)	604
	分子量	N/A	溶剂	Water
	产品详细介绍

简要概述

产品说明书

样品分析方案

溶液配制

储备溶液配制 

iFluor 594 小麦胚芽凝集素 (WGA) 缀合物储备液 (200X)

将 500 µL ddH₂O 添加到粉末小瓶中，制成 2 mg/mL 的储备溶液。 注意：重新配制的缀合物溶液可在 2-8 °C 下短期储存，或在 -20 °C 下长期储存。

工作溶液配制 

iFluor 594-小麦胚芽凝集素 (WGA) 缀合物​​工作溶液 (1X)

将 5 μL 200X WGA 储备溶液添加到 1 mL HHBS 缓冲液中。
注意：不同细胞系的优化染色浓度可能不同。 活细胞的推荐起始浓度为 5-10 µg/mL。

操作步骤

1.活细胞染色
1.1 用 HHBS 缓冲液清洗细胞两次。
1.2 添加 100 µL iFluor 594-WGA 工作溶液。
1.3 将细胞与 WGA 工作溶液在 37°C 下孵育 10-30 分钟。
1.4 用 HHBS 缓冲液清洗细胞两次。
1.5 使用 Cy3/TRITC 滤光片组在荧光显微镜上成像细胞。

2.固定细胞染色
2.1 在PBS中使用4％的甲醛固定细胞。
注意：对于固定细胞膜染色，建议在没有透化步骤的情况下进行染色。固定后透化步骤会导致细胞内区室染色，如高尔基体和内质网 (ER) 结构染色。
2.2 添加 100 µL iFluor 594-WGA 工作溶液。
2.3 在室温下用 WGA 工作溶液孵育细胞 10-30 分钟。
2.4 用 HHBS 缓冲液清洗细胞两次。
2.5 使用 Cy3/TRITC 滤光片组在荧光显微镜上成像细胞。

图示

图 1. 使用 iFluor 594-小麦胚芽凝集素 (WGA) 缀合物​​以 5 µg/mL的浓度在活细胞（Hela细胞） 染色 30 分钟，然后用 Hoechst 33342（Cat# 17535）染色。最后使用带 Cy3/TRITC和DAPI滤波片组的荧光显微镜成像。

参考文献

Membrane-associated gamma-glutamyl transferase and alkaline phosphatase in the context of concanavalin A- and wheat germ agglutinin-reactive glycans mark seminal prostasome populations from normozoospermic and oligozoospermic men.
Authors: Janković, Tamara and Goč, Sanja and Mitić, Ninoslav and Danilović Luković, Jelena and Janković, Miroslava
Journal: Upsala journal of medical sciences (2020): 10-18

Succinylated Wheat Germ Agglutinin Colocalizes with the Toxoplasma gondii Cyst Wall Glycoprotein CST1.
Authors: Guevara, Rebekah B and Fox, Barbara A and Bzik, David J
Journal: mSphere (2020)

Wheat germ agglutinin is a biomarker of whole grain content in wheat flour and pasta.
Authors: Killilea, David W and McQueen, Rebecca and Abegania, Judi R
Journal: Journal of food science (2020): 808-815

Wheat germ agglutinin is involved in the protective action of 24-epibrassinolide on the roots of wheat seedlings under drought conditions.
Authors: Avalbaev, Azamat and Bezrukova, Marina and Allagulova, Chulpan and Lubyanova, Alsu and Kudoyarova, Guzel and Fedorova, Kristina and Maslennikova, Dilara and Yuldashev, Ruslan and Shakirova, Farida
Journal: Plant physiology and biochemistry : PPB (2020): 420-427

Wheat germ agglutinin liposomes with surface grafted cyclodextrins as bioadhesive dual-drug delivery nanocarriers to treat oral cells.
Authors: Wijetunge, Sashini S and Wen, Jianchuan and Yeh, Chih-Ko and Sun, Yuyu
Journal: Colloids and surfaces. B, Biointerfaces (2020): 110572

Wheat germ agglutinin-conjugated fluorescent pH sensors for visualizing proton fluxes.
Authors: Zhang, Lejie and Zhang, Mei and Bellve, Karl and Fogarty, Kevin E and Castro, Maite A and Brauchi, Sebastian and Kobertz, William R
Journal: The Journal of general physiology (2020)

Bacterial expression, purification and biophysical characterization of wheat germ agglutinin and its four hevein-like domains.
Authors: Leyva, Eduardo and Medrano-Cerano, Jorge L and Cano-Sánchez, Patricia and López-González, Itzel and Gómez-Velasco, Homero and Del Río-Portilla, Federico and García-Hernández, Enrique
Journal: Biopolymers (2019): e23242

Enhanced anti-colon cancer efficacy of 5-fluorouracil by epigallocatechin-3- gallate co-loaded in wheat germ agglutinin-conjugated nanoparticles.
Authors: Wang, Ruoning and Huang, Jinyu and Chen, Jian and Yang, Mengmeng and Wang, Honglan and Qiao, Hongzhi and Chen, Zhipeng and Hu, Lihong and Di, Liuqing and Li, Junsong
Journal: Nanomedicine : nanotechnology, biology, and medicine (2019): 102068

Targeted delivery of etoposide, carmustine and doxorubicin to human glioblastoma cells using methoxy poly(ethylene glycol)‑poly(ε‑caprolactone) nanoparticles conjugated with wheat germ agglutinin and folic acid.
Authors: Kuo, Yung-Chih and Chang, Yu-Hsuan and Rajesh, Rajendiran
Journal: Materials science & engineering. C, Materials for biological applications (2019): 114-128

Towards the preparation of synthetic outer membrane vesicle models with micromolar affinity to wheat germ agglutinin using a dialkyl thioglycoside.
Authors: Fayolle, Dimitri and Berthet, Nathalie and Doumeche, Bastien and Renaudet, Olivier and Strazewski, Peter and Fiore, Michele
Journal: Beilstein journal of organic chemistry (2019): 937-946

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