1X4 Color V405染料套装

	货号	8000	存储条件	在零下15度以下保存, 避免光照
	规格	1 Set	价格	11988
	Ex (nm)		Em (nm)
	分子量		溶剂
	产品详细介绍

简要概述

产品基本信息

货号：8000

产品名称：1X4 Color V405染料套装

规格：1 Set

储存条件：-15℃避光防潮

保质期：24个月

产品介绍

1X4 Color V405染料套装为多色应用提供了一组高荧光染料。该组中的所有4种染料均被紫激光（405 nm）很好地激发，分别在450、500、550和610 nm处有4种不同的发射光。这种独特的染料组使具有单个激光器（例如，紫罗兰色激光器或LED）的荧光仪器和设备实现了多色检测。

参考文献

Cultivating DNA Sequencing Technology After the Human Genome Project.
Authors: Schloss, Jeffery A and Gibbs, Richard A and Makhijani, Vinod B and Marziali, Andre
Journal: Annual review of genomics and human genetics (2020): 117-138

Evaluation of PCR conditions for characterizing bacterial communities with full-length 16S rRNA genes using a portable nanopore sequencer.
Authors: Fujiyoshi, So and Muto-Fujita, Ai and Maruyama, Fumito
Journal: Scientific reports (2020): 12580

microRNA and exosome profiling in multiple sclerosis.
Authors: Mycko, Marcin P and Baranzini, Sergio E
Journal: Multiple sclerosis (Houndmills, Basingstoke, England) (2020): 599-604

Identification and Clinical Implications of a Novel MYO15A Variant in a Consanguineous Iranian Family by Targeted Exome Sequencing.
Authors: Zarepour, Narges and Koohiyan, Mahbobeh and Taghipour-Sheshdeh, Afsaneh and Nemati-Zargaran, Fatemeh and Saki, Nader and Mohammadi-Asl, Javad and Tabatabaiefar, Mohammad Amin and Hashemzadeh-Chaleshtori, Morteza
Journal: Audiology & neuro-otology (2019): 25-31

Overview of High-Throughput Cloning Methods for the Post-genomic Era.
Authors: Ortega, Claudia and Abreu, Cecilia and Oppezzo, Pablo and Correa, Agustín
Journal: Methods in molecular biology (Clifton, N.J.) (2019): 3-32

The Role of the Gut Microbiome in Multiple Sclerosis Risk and Progression: Towards Characterization of the “MS Microbiome”.
Authors: Pröbstel, Anne-Katrin and Baranzini, Sergio E
Journal: Neurotherapeutics : the journal of the American Society for Experimental NeuroTherapeutics (2018): 126-134

Best practices for germ-free derivation and gnotobiotic zebrafish husbandry.
Authors: Melancon, E and Gomez De La Torre Canny, S and Sichel, S and Kelly, M and Wiles, T J and Rawls, J F and Eisen, J S and Guillemin, K
Journal: Methods in cell biology (2017): 61-100

Differential Disease Susceptibilities in Experimentally Reptarenavirus-Infected Boa Constrictors and Ball Pythons.
Authors: Stenglein, Mark D and Sanchez-Migallon Guzman, David and Garcia, Valentina E and Layton, Marylee L and Hoon-Hanks, Laura L and Boback, Scott M and Keel, M Kevin and Drazenovich, Tracy and Hawkins, Michelle G and DeRisi, Joseph L
Journal: Journal of virology (2017)

MYO15A splicing mutations in hearing loss: A review literature and report of a novel mutation.
Authors: Motavaf, Mahsa and Soveizi, Mahdieh and Maleki, Majid and Mahdieh, Nejat
Journal: International journal of pediatric otorhinolaryngology (2017): 35-38

Mechanisms of Surface Antigenic Variation in the Human Pathogenic Fungus Pneumocystis jirovecii.
Authors: Schmid-Siegert, Emanuel and Richard, Sophie and Luraschi, Amanda and Mühlethaler, Konrad and Pagni, Marco and Hauser, Philippe M
Journal: mBio (2017)

Helixyte Green双链DNA定量检测试剂盒

简要概述

DNA定量是DNA样品制备过程中的一项重要工作，Helixyte 绿色双链DNA定量检测试剂盒提供了一种用Helixyte Green BR快速测定dsDNA的方法。该测定法在三个数量级上是线性的，并且比紫外线吸收度读数灵敏度高几个数量级。Helixyte Green-BR与dsDNA结合后荧光增强，序列依赖性小，可准确测定基因组DNA、病毒DNA、miniprep-DNA或PCR扩增产物等多种来源的DNA样品。该方法对RNA上的双链DNA（dsDNA）具有高度的选择性，并且被优化以测量从10 pg/ul到10 ng/ul的DNA浓度。金畔生物是AAT Bioquest的中国代理商，为您提供最优质的Helixyte 绿色双链DNA定量检测试剂盒。

产品说明书

样品实验方案

简要概述

1. 准备dSDNA标准品，测试样品和染料工作溶液
2. 添加DNA标准液或测试样品（50 uL）
3. 添加Helixyte Green-BR工作溶液（50 uL）
4. 在室温下孵育2分钟
5. 检测Ex / Em = 490/530 nm的荧光强度

提示：操作前将所有组件加热到室温。暂时没有关于Helixyte Green dsDNA染色剂的致突变性或毒性的数据。由于该试剂与核酸结合，因此应将其视为潜在的诱变剂，应谨慎处理。DMSO储备溶液应特别小心，因为已知DMSO有助于有机分子进入组织。

 

溶液配制

标准溶液配制

将100 uL的100 ug / mL dsDNA标准溶液（组分C）添加到400 uL的测定缓冲液（组分B）中，以生成20 ug / mL的dsDNA标准溶液。然后用测定缓冲液（组分B）进行1：3的系列稀释，以得到0至20 ug / mL的系列稀释的dsDNA标准品。

 

工作溶液配制

Helixyte Green BR工作溶液：将50 uL Helixyte Green BR（组分A）添加到5 mL的测定缓冲液（组分B）中，使总体积为5.050 mL。通过用箔纸覆盖或将其置于黑暗中来保护工作溶液免受光照。 注意：我们建议在塑料容器中而不是玻璃中制备该溶液，因为染料可能会吸附到玻璃表面。为了获得最佳结果，应在准备后的几个小时内使用此溶液。

 

实验步骤

表1.  在透明底部96孔微孔板中的dsDNA标准品和测试样品的布局。DS = dsDNA标准品（DS1-DS7，20至0.027 ug / mL）; BL =空白对照；TS =测试样品

BL	BL	TS	TS
DS1	DS1	…	…
DS2	DS2	…	…
DS3	DS3
DS4	DS4
DS5	DS5
DS6	DS6
DS7	DS7

表2.  每个孔的试剂组成

Well	Volume	Reagent
DS1-DS7	50 uL	连续稀释 (20 to 0.027 ug/mL)
BL	50 uL	缓冲液 (Component B)
TS	50 uL	实验样品

1. 根据表1和表2提供的布局，制备dsDNA标准品（DS）、空白对照品（BL）和测试样品（TS）。对于384孔板，每孔使用25 uL试剂，而不是50 uL。注：根据需要处理细胞或组织样本。
   
2. 添加50 uL Helixyte Green-BR染料工作液（2X）分别加入dsDNA标准液、空白对照液和供试品中，使总分析体积为100ul/孔。对于384孔板，添加25 uL的Helixyte Green-BR染料工作溶液，每孔总体积为50 uL/孔。
   
3. 在室温下避光培养2分钟。
   
4. 在Ex/Em=490/530 nm（截止=515nm）处用荧光酶标仪检测荧光强度。

 

图示

 

 

图1.用Helixyte Green dsDNA定量试剂盒在96孔黑色孔板中测量了dsDNA剂量反应。

 

 

参考文献

A new reporter design based on DNA origami nanostructures for quantification of short oligonucleotides using microbeads
Authors: Choi, Y., Schmidt, C., Tinnefeld, P., Bald, I., Rodiger, S.
Journal: Sci Rep (2019): 4769

A universal fluorescence-based toolkit for real-time quantification of DNA and RNA nuclease activity
Authors: Sheppard, E. C., Rogers, S., Harmer, N. J., Chahwan, R.
Journal: Sci Rep (2019): 8853

Effects of Quantification Methods, Isolation Kits, Plasma Biobanking, and Hemolysis on Cell-Free DNA Analysis in Plasma
Authors: Streleckiene, G., Forster, M., Inciuraite, R., Lukosevicius, R., Skieceviciene, J.
Journal: Biopreserv Biobank (2019): ersion=”1.0″ encoding=”UTF-8″ ?>17645.enlEndNote1117Streleckiene, G.Forster, M.Inciuraite, R.Lukosevicius, R.Skieceviciene, J.1Institute for Digestive Research, Lithuanian University of Health Sciences, Kaunas, Lithuania. 2Institute of Clinical Molecu

Flat-top TIRF illumination boosts DNA-PAINT imaging and quantification
Authors: Stehr, F., Stein, J., Schueder, F., Schwille, P., Jungmann, R.
Journal: Nat Commun (2019): 1268

Molecular-Recognition-Based DNA Nanodevices for Enhancing the Direct Visualization and Quantification of Single Vesicles of Tumor Exosomes in Plasma Microsamples
Authors: He, D., Ho, S. L., Chan, H. N., Wang, H., Hai, L., He, X., Wang, K., Li, H. W.
Journal: Anal Chem (2019): 2768-2775

Quantification of fixed adherent cells using a strong enhancer of the fluorescence of DNA dyes
Authors: Ligasova, A., Koberna, K.
Journal: Sci Rep (2019): 8701

A fluorescent reporter for quantification and enrichment of DNA editing by APOBEC-Cas9 or cleavage by Cas9 in living cells
Authors: St Martin, A., Salamango, D., Serebrenik, A., Shaban, N., Brown, W. L., Donati, F., Munagala, U., Conticello, S. G., Harris, R. S.
Journal: Nucleic Acids Res (2018): e84

Accuracy of human sperm DNA oxidation quantification and threshold determination using an 8-OHdG immuno-detection assay
Authors: Vorilhon, S., Brugnon, F., Kocer, A., Dollet, S., Bourgne, C., Berger, M., Janny, L., Pereira, B., Aitken, R. J., Moazamian, A., Gharagozloo, P., Drevet, J., Pons-Rejraji, H.
Journal: Hum Reprod (2018): 553-562

Cell Type-Specific Quantification of Telomere Length and DNA Double-strand Breaks in Individual Lung Cells by Fluorescence In Situ Hybridization and Fluorescent Immunohistochemistry
Authors: van Batenburg, A. A., Kazemier, K. M., Peeters, T., van Oosterhout, M. F. M., van der Vis, J. J., Grutters, J. C., Goldschmeding, R., van Moorsel, C. H. M.
Journal: J Histochem Cytochem (2018): 485-495

Identification and Quantification of Heterogeneously-methylated DNA Fragments Using Epiallele-sensitive Droplet Digital Polymerase Chain Reaction (EAST-ddPCR)
Authors: Menschikowski, M., J and eck, C., Friedemann, M., Richter, S., Thiem, D., Lange, B. S., Suttorp, M.
Journal: Cancer Genomics Proteomics (2018): 299-312

1X4 Color B488染料套装

	货号	8005	存储条件	在零下15度以下保存, 避免光照
	规格	1 Set	价格	11988
	Ex (nm)		Em (nm)
	分子量		溶剂
	产品详细介绍

简要概述

产品基本信息

货号：8005

产品名称：1X4 Color B488染料套装

规格：1 Set

储存条件：-15℃避光防潮

保质期：24个月

产品介绍

1X4 Color B488染料套装为多色应用提供了一组高荧光染料。该组中的所有4种染料均被氩激光（488 nm）充分激发，分别在520、560、590和630 nm处有4种不同的发射光。这种独特的染料组使具有单个激光器（例如，氩激光器或LED）的荧光仪器和设备实现了多色检测。

参考文献

BEON: A Functional Fluorescence Reporter for Quantification and Enrichment of Adenine Base-Editing Activity.
Authors: Wang, Peipei and Xu, Li and Gao, Yandi and Han, Renzhi
Journal: Molecular therapy : the journal of the American Society of Gene Therapy (2020): 1696-1705

DNA bioassays based on the fluorescence ‘turn off’ of silver nanocluster beacon.
Authors: Wen, Qiu-Lin and Peng, Jun and Liu, An-Yong and Wang, Jun and Hu, Yi-Lin and Ling, Jian and Cao, Qiu-E
Journal: Luminescence : the journal of biological and chemical luminescence (2020): 702-708

Advancing Wireframe DNA Nanostructures Using Single-Molecule Fluorescence Microscopy Techniques.
Authors: Platnich, Casey M and Hariri, Amani A and Sleiman, Hanadi F and Cosa, Gonzalo
Journal: Accounts of chemical research (2019): 3199-3210

Approach to molecular subgrouping of medulloblastomas: Comparison of NanoString nCounter assay versus combination of immunohistochemistry and fluorescence in-situ hybridization in resource constrained centres.
Authors: Kaur, Kavneet and Jha, Prerana and Pathak, Pankaj and Suri, Vaishali and Sharma, Mehar Chand and Garg, Ajay and Suri, Ashish and Sarkar, Chitra
Journal: Journal of neuro-oncology (2019): 393-403

Determination of the activity of uracil-DNA glycosylase by using two-tailed reverse transcription PCR and gold nanoparticle-mediated silver nanocluster fluorescence: a new method for gene therapy-related enzyme detection.
Authors: Zhang, Kai and Huang, Wanting and Huang, Yue and Wang, Ke and Zhu, Xue and Xie, Minhao
Journal: Mikrochimica acta (2019): 181

Fluorescent sensor for detection of miR-141 based on target-induced fluorescence enhancement and PicoGreen.
Authors: Lavaee, Parirokh and Taghdisi, Seyed Mohammad and Abnous, Khalil and Danesh, Noor Mohammad and Khayyat, Ladan Hassanzadeh and Jalalian, Seyed Hamid
Journal: Talanta (2019): 349-353

High Sensitivity Measurement of Transcription Factor-DNA Binding Affinities by Competitive Titration Using Fluorescence Microscopy.
Authors: Jung, Christophe and Schnepf, Max and Bandilla, Peter and Unnerstall, Ulrich and Gaul, Ulrike
Journal: Journal of visualized experiments : JoVE (2019)

Influence of gold nanoparticles in different aggregation states on the fluorescence of carbon dots and its application.
Authors: Qin, Xuefei and Lu, Yuping and Bian, Mengmeng and Xiao, Zhourui and Zhang, Yun and Yuan, Yali
Journal: Analytica chimica acta (2019): 119-126

Insertion/deletion-activated frame-shift fluorescence protein is a sensitive reporter for genomic DNA editing.
Authors: Kumar, Akhilesh and Birnbaum, Michael D and Moorthy, Balaji T and Singh, Jayanti and Palovcak, Anna and Patel, Devang M and Zhang, Fangliang
Journal: BMC genomics (2019): 609

DNA assay based on Nanoceria as Fluorescence Quenchers (NanoCeracQ DNA assay).
Authors: Bülbül, Gonca and Hayat, Akhtar and Mustafa, Fatima and Andreescu, Silvana
Journal: Scientific reports (2018): 2426

Amplite 比色法丁酰胆碱酯酶活性检测试剂盒

简要概述

丁酰胆碱酯酶（EC 3.1.1.8； BChE），也称为假胆碱酯酶或血浆胆碱酯酶，主要在肝脏中合成，并存在于血液中。BChE是一种非特异性胆碱酯酶，可以水解许多不同的胆碱酯，是抵御有毒化合物进入血液的第一道防线。它已被鉴定为有机磷酸中毒的临床生物标志物。Amplite 比色丁酰胆碱酯酶活性测定试剂盒是合成的丁酰胆碱酯类底物，可被BChE水解并产生硫代胆碱。硫胆碱可与5、5′-二硫代双（2-硝基苯甲酸）（DTNB）反应，并生成黄色发色团，可在410 nm处检测到。该测定方便，灵敏，并且在各种样品中均可检测到低至6 mU / mL。金畔生物是AAT Bioquest的中国代理商，为您提供最优质的Amplite 比色法丁酰胆碱酯酶活性检测试剂盒。

产品说明书

样品实验方案

简要概述

1. 准备BChE标准品，测试样品和染料工作溶液
2. 添加BChE标准品或测试样品（100 uL）
3. 添加BChE染料工作溶液（100 uL）
4. 在室温下孵育10-30分钟
5. 检测410±5 nm处的吸光度

注意：开始实验前，在室温下解冻试剂盒所有成分。

 

溶液配制

储备溶液配制

1.DTNB储备溶液（10倍）：将1.2 mL测定缓冲液（组分B）添加到DTNB（组分A）小瓶中，制成10X DTNB储备溶液，避光。 注意：DNTB不易溶解，看到溶液混浊是正常的。 可以将上清液或混合物用于实验。

2.丁硫代胆碱（BTC）储备溶液（100X）：将120 uL ddH₂O加入BTC小瓶（组分C）中，制成100X BTC储备液。

3.丁胆碱酯酶（BChE）标准溶液（20 U / mL）：将50 uL含0.1％BSA的ddH₂O添加到小瓶丁酰胆碱酯酶标准溶液（组分D）中，制成20 U / mL丁酰胆碱酯酶标准溶液。

 

标准溶液配制

将20 uL的20 U / mL BChE标准溶液添加到980 uL的测定缓冲液（组分B）中以生成400 mU / mL的BChE标准溶液（BS1）。 然后取400 mU / mL BChE标准溶液（BS7），并在测定缓冲液（组分B）中进行1：2连续稀释，以得到连续稀释的BChE标准溶液（BS2- BS7）。 注意：稀释的BChE标准溶液不稳定，应在4小时内使用。

 

工作溶液配制

BChE染料工作溶液：将1.0 mL的10 X DTNB储备溶液和100μL的100X BTC储备溶液添加到9 mL的测定缓冲液（组分B）中，使总体积为10.1 mL的BChE染料工作溶液，避光。

 

实验步骤

表1.透明的96孔微孔板中的BChE标准品和测试样品的布局。 BS = BChE标准品（BS1-BS7，400至6.5 mU / mL）; BL =空白控件； TS =测试样品

BL	BL	TS	TS
DS1	DS1	…	…
DS2	DS2	…	…
DS3	DS3
DS4	DS4
DS5	DS5
DS6	DS6
DS7	DS7

表2.  每个孔的试剂组成

Well	Volume	Reagent
DS1-DS7	100 uL	连续稀释 (400 to 6.5 mU/mL)
BL	100 uL	缓冲液 (Component B)
TS	100 uL	实验样品

1. 根据表1和表2提供的布局，准备BChE标准品（BS），空白对照（BL）和测试样品（TS）。对于384孔板，每孔使用25 uL的试剂代替100 uL。 注意：根据需要处理细胞或组织样品。

2. 将100 uL BChE染料工作溶液添加到BChE标准品，空白对照和测试样品的每个孔中，使总测定体积为200 uL /孔。 对于384孔板，将25 uL BChE工作溶液添加到每个孔中，总体积为50 uL /孔。

3. 在避光条件下，于室温下孵育反应10至30分钟。

4. 用酶标仪在410±5 nm处检测吸光度的增加。

 

图示

 

图1.使用SpectraMax酶标仪在白色/透明底96孔板中使用Amplite 比色丁酰胆碱酯酶测定试剂盒测量丁酰胆碱酯酶的剂量响应。 孵育10分钟可检测到低至6.0 mU / mL的丁酰胆碱酯酶（n = 3）。

 

参考文献

A randomized crossover trial assessing the effects of acute exercise on appetite, circulating ghrelin concentrations, and butyrylcholinesterase activity in normal-weight males with variants of the obesity-linked FTO rs9939609 polymorphism
Authors: Dorling, J. L., Clayton, D. J., Jones, J., Carter, W. G., Thackray, A. E., King, J. A., Pucci, A., Batterham, R. L., Stensel, D. J.
Journal: Am J Clin Nutr (2019): ersion=”1.0″ encoding=”UTF-8″ ?>11406.enlEndNote1117Dorling, J. L.Clayton, D. J.Jones, J.Carter, W. G.Thackray, A. E.King, J. A.Pucci, A.Batterham, R. L.Stensel, D. J.National Centre for Sport and Exercise Medicine, School of Sport, Exercise, and Health S

Anti-Alzheimer’s multitarget-directed ligands with serotonin 5-HT6 antagonist, butyrylcholinesterase inhibitory, and antioxidant activity
Authors: Marcinkowska, M., Bucki, A., Panek, D., Siwek, A., Fajkis, N., Bednarski, M., Zygmunt, M., Godyn, J., Del Rio Valdivieso, A., Kotanska, M., Kolaczkowski, M., Wieckowska, A.
Journal: Arch Pharm (Weinheim) (2019): e1900041

Discovery of New Selective Butyrylcholinesterase (BChE) Inhibitors with Anti-Abeta Aggregation Activity: Structure-Based Virtual Screening, Hit Optimization and Biological Evaluation
Authors: Jiang, C. S., Ge, Y. X., Cheng, Z. Q., Wang, Y. Y., Tao, H. R., Zhu, K., Zhang, H.
Journal: Molecules (2019): se name=”11406.enl” path=”C:UsersaatbiDropbox (AAT Bioquest)Catalogs & Website Working FilesProduct References11406.enl”>11406.enlEndNote5517Jiang, C. S.Ge, Y. X.Cheng, Z. Q.Wang, Y. Y.Tao, H. R.Zhu, K.Zhang, H.School of Biological Science and T

Drop of Butyrylcholinesterase Activity after Cyclophosphamide Conditioning as a Predictive Marker of Liver Transplant-Related Complications and Its Correlation with Transplant-Related Mortality in Pediatric Hematopoietic Stem Cell Recipients
Authors: Maximova, N., Caddeo, G., Zanon, D., Maestro, A., Simeone, R.
Journal: J Clin Med (2019): se name=”11406.enl” path=”C:UsersaatbiDropbox (AAT Bioquest)Catalogs & Website Working FilesProduct References11406.enl”>11406.enlEndNote6617Maximova, N.Caddeo, G.Zanon, D.Maestro, A.Simeone, R.Bone Marrow Transplant Unit, Institute for Maternal

Effects of simvastatin and fenofibrate on butyrylcholinesterase activity in the brain, plasma, and liver of normolipidemic and hyperlipidemic rats
Authors: Vuksic, A., Lovric, J., Konjevoda, P., Blazevic, N., Bilusic, M., Bradamante, V.
Journal: Arh Hig Rada Toksikol (2019): 30-35

Flavonols and 4-thioflavonols as potential acetylcholinesterase and butyrylcholinesterase inhibitors: Synthesis, structure-activity relationship and molecular docking studies
Authors: Mughal, E. U., Sadiq, A., Ashraf, J., Zafar, M. N., Sumrra, S. H., Tariq, R., Mumtaz, A., Javid, A., Khan, B. A., Ali, A., Javed, C. O.
Journal: Bioorg Chem (2019): 103124

Haplotypes of butyrylcholinesterase K-variant and their influence on the enzyme activity
Authors: Jasiecki, J., Zuk, M., Krawczynska, N., Jonca, J., Szczoczarz, A., Lew and owski, K., Waleron, K., Wasag, B.
Journal: Chem Biol Interact (2019): 154-157

Microwave-Assisted Organic Synthesis, structure-activity relationship, kinetics and molecular docking studies of non-cytotoxic benzamide derivatives as selective butyrylcholinesterase inhibitors
Authors: Wajid, S., Khatoon, A., Khan, M. A., Zafar, H., Kanwal, S., Atta Ur, Rahman, Choudhary, M. I., Basha, F. Z.
Journal: Bioorg Med Chem (2019): 4030-4040

Phytochemical content, antioxidant activity, and enzyme inhibition effect of Salvia eriophora Boiss. & Kotschy against acetylcholinesterase, alpha-amylase, butyrylcholinesterase, and alpha-glycosidase enzymes
Authors: Bursal, E., Aras, A., Kilic, O., Taslimi, P., Goren, A. C., Gulcin, I.
Journal: J Food Biochem (2019): e12776

Profiling Auspicious Butyrylcholinesterase Inhibitory Activity of Two Herbal Molecules: Hyperforin and Hyuganin C
Authors: Orhan, I. E., Senol Deniz, F. S., Traedal-Henden, S., Ceron-Carrasco, J. P., den Haan, H., Pena-Garcia, J., Perez-Sanchez, H., Emerce, E., Skalicka-Wozniak, K.
Journal: Chem Biodivers (2019): e1900017

AF 488醛

	货号	9015	存储条件	在零下15度以下保存, 避免光照
	规格	5 mg	价格	4992
	Ex (nm)	499	Em (nm)	520
	分子量	750.75	溶剂	DMSO
	产品详细介绍

简要概述

产品基本信息

货号：9015

产品名称：AF 488醛

规格：5 mg

储存条件：-15℃避光防潮

保质期：24个月

产品物理化学光谱特性

分子量：639.02

溶剂：DMSO

激发波长(nm)：499

发射波长(nm)：520

消光系数（cm-1 M-1）:73000

荧光量子产率：0.92

校正因子 (280 nm)：0.11

产品介绍

AF488醛包含AlexaFluor®488的荧光团。它是一种可与胺，肼或羟胺反应的活性绿色荧光染料。醛基可与pH 5-9的胺，酰肼或羟胺基反应。与胺反应性琥珀酰亚胺基酯基（NHS）不同，醛可以在酸性pH下与N端胺基反应，这是某些生物共轭反应有时需要的条件。醛与胺基反应形成席夫碱。用氢化物进一步还原将形成稳定的C-N键。醛与其他基团之间的反应可实现荧光素染料的位点特异性缀合和标记，使其靶向分子上的所需位置。可以通过普通的荧光仪器在FITC通道下轻松检测到共轭AF488染料。 AlexaFluor®488是ThermoFisher的商标。

点击查看光谱

参考文献

Optimization of 3-aminopropyltriethoxysilane functionalization on silicon nitride surface for biomolecule immobilization.
Authors: Saengdee, Pawasuth and Promptmas, Chamras and Thanapitak, Surachoke and Srisuwan, Awirut and Pankiew, Apirak and Thornyanadacha, Nutthaphat and Chaisriratanakul, Woraphan and Chaowicharat, Ekalak and Jeamsaksiri, Wutthinan
Journal: Talanta (2020): 120305

Alexa fluor-labeled fluorescent cellulose nanocrystals for bioimaging solid cellulose in spatially structured microenvironments.
Authors: Grate, Jay W and Mo, Kai-For and Shin, Yongsoon and Vasdekis, Andreas and Warner, Marvin G and Kelly, Ryan T and Orr, Galya and Hu, Dehong and Dehoff, Karl J and Brockman, Fred J and Wilkins, Michael J
Journal: Bioconjugate chemistry (2015): 593-601

Simultaneous visualization and cell-specific confirmation of RNA and protein in the mouse retina.
Authors: Stempel, Andrew J and Morgans, Catherine W and Stout, J Timothy and Appukuttan, Binoy
Journal: Molecular vision (2014): 1366-73

Amine-reactive pyridylhydrazone-based PEG reagents for pH-reversible PEI polyplex shielding.
Authors: Fella, Carolin and Walker, Greg F and Ogris, Manfred and Wagner, Ernst
Journal: European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences (2008): 309-20

Raman nanoparticle probes for antibody-based protein detection in tissues.
Authors: Lutz, Barry and Dentinger, Claire and Sun, Lei and Nguyen, Lienchi and Zhang, Jingwu and Chmura, Aj and Allen, April and Chan, Selena and Knudsen, Beatrice
Journal: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society (2008): 371-9

Rapid oxime and hydrazone ligations with aromatic aldehydes for biomolecular labeling.
Authors: Dirksen, Anouk and Dawson, Philip E
Journal: Bioconjugate chemistry (2008): 2543-8

Oligonucleotide-arrayed TFT photosensor applicable for DNA chip technology.
Authors: Tanaka, Tsuyoshi and Hatakeyama, Keiichi and Sawaguchi, Masahiro and Iwadate, Akihito and Mizutani, Yasushi and Sasaki, Kazuhiro and Tateishi, Naofumi and Takeyama, Haruko and Matsunaga, Tadashi
Journal: Biotechnology and bioengineering (2006): 22-8

Use of anti-fluorophore antibody to achieve high-sensitivity immunolocalizations of transporters and ion channels.
Authors: Coleman, Richard A and Liu, Jie and Wade, James B
Journal: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society (2006): 817-27

Acid-induced conformational changes in phosphoglucose isomerase result in its increased cell surface association and deposition on fibronectin fibrils.
Authors: Amraei, Mohammad and Jia, Zongjian and Reboul, Pascal and Nabi, Ivan R
Journal: The Journal of biological chemistry (2003): 38935-41

Lipopolysaccharide (LPS) labeled with Alexa 488 hydrazide as a novel probe for LPS binding studies.
Authors: Triantafilou, K and Triantafilou, M and Fernandez, N
Journal: Cytometry (2000): 316-20

Amplite 荧光法丁酰胆碱酯酶活性检测试剂盒

简要概述

丁酰胆碱酯酶（EC 3.1.1.8； BChE），也称为假胆碱酯酶或血浆胆碱酯酶，主要在肝脏中合成，并存在于血液中。BChE是一种非特异性胆碱酯酶，可以水解许多不同的胆碱酯，是抵御有毒化合物进入血液的第一道防线。它已被鉴定为有机磷酸中毒的临床生物标志物。Amplite 比色丁酰胆碱酯酶活性测定试剂盒是合成的丁酰胆碱酯类底物，可被BChE水解并产生硫代胆碱。硫胆碱可与5、5′-二硫代双（2-硝基苯甲酸）（DTNB）反应，并生成黄色发色团，可在410 nm处检测到。该测定方便，灵敏，并且在各种样品中均可检测到低至6 mU / mL。金畔生物是AAT Bioquest的中国代理商，为您提供最优质的Amplite 荧光法丁酰胆碱酯酶活性检测试剂盒。

产品说明书

样品实验方案

简要概述

1. 准备BChE标准品，测试样品和染料工作溶液
2. 添加BChE标准品或测试样品（100 uL）
3. 添加BChE染料工作溶液（100 uL）
4. 在室温下孵育10-30分钟
5. 检测Ex / Em = 490/525nm的荧光强度

注意：开始实验前，在室温下解冻试剂盒所有成分。

 

溶液配制

储备溶液配制

1.Thiolite 绿色原液（200X）：将50 uL DMSO（组分E）加入小瓶Thiolite Green（组分A）中，制成200X Thiolite Green储备溶液。

2.丁硫代胆碱（BTC）储备溶液（100X）：将120 uL ddH2O加入BTC小瓶（组分C）中，制成100X BTC储备液。

3.丁胆碱酯酶（BChE）标准溶液（5 U / mL）：将50 uL含0.1％BSA的ddH₂O加入BChE标准品（组分D）小瓶中，制成5 U / mL BChE标准品溶液。

 

标准溶液配制

将20 uL的5U / mL BChE标准溶液添加到980 uL的测定缓冲液（组分B）中，以生成100mU / mL的BChE标准溶液（BS1），然后取100 mU / mL的BChE标准溶液（BS1），并在测定缓冲液（组分B）中进行1：3连续稀释，以得到系列换算的BChE标准溶液（BS2-BS7）。注意：减少的BChE标准溶液溶液，应在4小时内使用。

 

工作溶液配制

BChE染料工作溶液：将50 uL的200X Thiolite Green储备溶液和100 uL的100X BTC储备溶液添加到10 mL的测定缓冲液（组分B）中，使总体积为10.1 mL BChE染料工作溶液。 注意：避光。

 

实验步骤

表1.在透明的96微孔板中的BChE标准品和测试样品的布局。 BS = BChE标准品（BS1-BS7，100至0.13 mU / mL）; BL =空白对照； TS =测试样品

BL	BL	TS	TS
DS1	DS1	…	…
DS2	DS2	…	…
DS3	DS3
DS4	DS4
DS5	DS5
DS6	DS6
DS7	DS7

表2.  每个孔的试剂组成

Well	Volume	Reagent
DS1-DS7	100 uL	连续稀释 (100 to 0.13 mU/mL)
BL	100 uL	缓冲液 (Component B)
TS	100 uL	实验样品

1. 根据表1和表2提供的布局，准备BChE标准品（BS），空白对照（BL）和测试样品（TS）。对于384孔板，每孔使用25 uL的试剂代替100 uL。 注意：根据需要处理细胞或组织样品。

2. 将100 uL BChE染料工作溶液添加到BChE标准品，空白对照和测试样品的每个孔中，使总测定体积为200 uL /孔。 对于384孔板，将25 uL BChE工作溶液添加到每个孔中，总体积为50 uL /孔。

3. 在避光条件下，于室温下孵育反应10至30分钟。

4. 使用荧光酶标仪在Ex / Em = 490/525nm处检测荧光的增加。

 

图示

 

图1.使用Gemini荧光酶标仪在带有Amplite 荧光丁酰胆碱酯酶检测试剂盒的黑色96孔板上测定丁酰胆碱酯酶的剂量响应。 孵育10分钟可检测到低至0.1 mU / mL的丁酰胆碱酯酶。

 

参考文献

A randomized crossover trial assessing the effects of acute exercise on appetite, circulating ghrelin concentrations, and butyrylcholinesterase activity in normal-weight males with variants of the obesity-linked FTO rs9939609 polymorphism
Authors: Dorling, J. L., Clayton, D. J., Jones, J., Carter, W. G., Thackray, A. E., King, J. A., Pucci, A., Batterham, R. L., Stensel, D. J.
Journal: Am J Clin Nutr (2019): ersion=”1.0″ encoding=”UTF-8″ ?>11406.enlEndNote1117Dorling, J. L.Clayton, D. J.Jones, J.Carter, W. G.Thackray, A. E.King, J. A.Pucci, A.Batterham, R. L.Stensel, D. J.National Centre for Sport and Exercise Medicine, School of Sport, Exercise, and Health S

Anti-Alzheimer’s multitarget-directed ligands with serotonin 5-HT6 antagonist, butyrylcholinesterase inhibitory, and antioxidant activity
Authors: Marcinkowska, M., Bucki, A., Panek, D., Siwek, A., Fajkis, N., Bednarski, M., Zygmunt, M., Godyn, J., Del Rio Valdivieso, A., Kotanska, M., Kolaczkowski, M., Wieckowska, A.
Journal: Arch Pharm (Weinheim) (2019): e1900041

Discovery of New Selective Butyrylcholinesterase (BChE) Inhibitors with Anti-Abeta Aggregation Activity: Structure-Based Virtual Screening, Hit Optimization and Biological Evaluation
Authors: Jiang, C. S., Ge, Y. X., Cheng, Z. Q., Wang, Y. Y., Tao, H. R., Zhu, K., Zhang, H.
Journal: Molecules (2019): se name=”11406.enl” path=”C:UsersaatbiDropbox (AAT Bioquest)Catalogs & Website Working FilesProduct References11406.enl”>11406.enlEndNote5517Jiang, C. S.Ge, Y. X.Cheng, Z. Q.Wang, Y. Y.Tao, H. R.Zhu, K.Zhang, H.School of Biological Science and T

Drop of Butyrylcholinesterase Activity after Cyclophosphamide Conditioning as a Predictive Marker of Liver Transplant-Related Complications and Its Correlation with Transplant-Related Mortality in Pediatric Hematopoietic Stem Cell Recipients
Authors: Maximova, N., Caddeo, G., Zanon, D., Maestro, A., Simeone, R.
Journal: J Clin Med (2019): se name=”11406.enl” path=”C:UsersaatbiDropbox (AAT Bioquest)Catalogs & Website Working FilesProduct References11406.enl”>11406.enlEndNote6617Maximova, N.Caddeo, G.Zanon, D.Maestro, A.Simeone, R.Bone Marrow Transplant Unit, Institute for Maternal

Effects of simvastatin and fenofibrate on butyrylcholinesterase activity in the brain, plasma, and liver of normolipidemic and hyperlipidemic rats
Authors: Vuksic, A., Lovric, J., Konjevoda, P., Blazevic, N., Bilusic, M., Bradamante, V.
Journal: Arh Hig Rada Toksikol (2019): 30-35

Flavonols and 4-thioflavonols as potential acetylcholinesterase and butyrylcholinesterase inhibitors: Synthesis, structure-activity relationship and molecular docking studies
Authors: Mughal, E. U., Sadiq, A., Ashraf, J., Zafar, M. N., Sumrra, S. H., Tariq, R., Mumtaz, A., Javid, A., Khan, B. A., Ali, A., Javed, C. O.
Journal: Bioorg Chem (2019): 103124

Haplotypes of butyrylcholinesterase K-variant and their influence on the enzyme activity
Authors: Jasiecki, J., Zuk, M., Krawczynska, N., Jonca, J., Szczoczarz, A., Lew and owski, K., Waleron, K., Wasag, B.
Journal: Chem Biol Interact (2019): 154-157

Microwave-Assisted Organic Synthesis, structure-activity relationship, kinetics and molecular docking studies of non-cytotoxic benzamide derivatives as selective butyrylcholinesterase inhibitors
Authors: Wajid, S., Khatoon, A., Khan, M. A., Zafar, H., Kanwal, S., Atta Ur, Rahman, Choudhary, M. I., Basha, F. Z.
Journal: Bioorg Med Chem (2019): 4030-4040

Phytochemical content, antioxidant activity, and enzyme inhibition effect of Salvia eriophora Boiss. & Kotschy against acetylcholinesterase, alpha-amylase, butyrylcholinesterase, and alpha-glycosidase enzymes
Authors: Bursal, E., Aras, A., Kilic, O., Taslimi, P., Goren, A. C., Gulcin, I.
Journal: J Food Biochem (2019): e12776

Profiling Auspicious Butyrylcholinesterase Inhibitory Activity of Two Herbal Molecules: Hyperforin and Hyuganin C
Authors: Orhan, I. E., Senol Deniz, F. S., Traedal-Henden, S., Ceron-Carrasco, J. P., den Haan, H., Pena-Garcia, J., Perez-Sanchez, H., Emerce, E., Skalicka-Wozniak, K.
Journal: Chem Biodivers (2019): e1900017

Buccutite AP（碱性磷酸酶）抗体偶联试剂盒*针对标记1 mg蛋白进行了优化*

简要概述

蛋白质-蛋白质缀合交联剂通常使用在每个末端具有不同反应性的双功能接头（例如SMCC）连接两个不同的蛋白质。交联剂的一端与赖氨酸和N端的胺（-NH2）反应（通过NHS酯），另一端与半胱氨酸的硫醇基（-SH）反应（通过马来酰亚胺）。但是，SMCC修饰的蛋白质极不稳定，通常会自反应，因为蛋白质包含多个胺基和硫醇基，会引起大量的交联。另外，定量蛋白质中的马来酰亚胺基团的数量是非常困难的。 Buccutite AP（碱性磷酸酶）抗体偶联试剂盒旨在快速制备具有预活化AP的AP偶联物。该试剂盒针对标记1mg蛋白进行了优化。 Buccutite FOL活化的AP可以在极其温和的中性条件下与Buccutite MTA修饰的抗体轻松反应，而无需任何催化剂。与常用的SMCC和其他类似技术相比，Buccutite 生物缀合系统更加稳定并且易于使用。它能够以更高的效率和产量更快且定量地偶联生物分子。

图示

图1.使用Buccutite AP（碱性磷酸酶）抗体偶联试剂盒制备的ALP-山羊-抗小鼠IgG偶联物检测了小鼠IgG剂量曲线。将3倍系列稀释的小鼠IgG包被在96孔板上，然后按照标准ELISA方法，向每个孔中加入100uL GAM IgG-ALP偶联物（1ug / ml）。在孵育30分钟后，将pNPP底物溶液用于检测固定的小鼠IgG，并在400 nm处读数。

参考文献

Direct pulp capping with mineral trioxide aggregate: an immunohistologic comparison with calcium hydroxide in rodents.
Authors: Dammaschke, Till and Stratmann, Udo and Wolff, Philipp and Sagheri, Darius and Schäfer, Edgar
Journal: Journal of endodontics (2010): 814-9

Vitamins C and E inhibit apoptosis of cultured human term placenta trophoblast.
Authors: Tannetta, D S and Sargent, I L and Linton, E A and Redman, C W G
Journal: Placenta (2008): 680-90

Anti-alkaline phosphatase antibody positive myasthenia gravis.
Authors: Konishi, Tetsuro and Ohta, Kiyoe and Shigemoto, Kazuhiro and Ohta, Mitsuhiro
Journal: Journal of the neurological sciences (2007): 89-93

Ectopic expression of alkaline phosphatase in proximal tubular brush border membrane of human renal cell carcinoma.
Authors: Prasad, R and Lambe, S and Kaler, P and Pathania, S and Kumar, S and Attri, S and Singh, S K
Journal: Biochimica et biophysica acta (2005): 240-5

[Identification of antigens of Colombian Giardia duodenalis isolates recognized by total IgG and subclasses].
Authors: Hernández, Jenny Fabiola and Duque, Sofía and Arévalo, Adriana and Guerrero, Rafael and Nicholls, Rubén Santiago
Journal: Biomedica : revista del Instituto Nacional de Salud (2003): 263-73

Primary intrapelvic seminoma in Klinefelter’s syndrome.
Authors: Kurabayashi, A and Furihata, M and Matsumoto, M and Sonobe, H and Ohtsuki, Y and Aki, M and Kuwahara, M
Journal: Pathology international (2001): 624-8

Recombinant adenoviral vector-lipofectAMINE complex for gene transduction into human T lymphocytes.
Authors: Di Nicola, M and Milanesi, M and Magni, M and Bregni, M and Carlo-Stella, C and Longoni, P and Tomanin, R and Ravagnani, F and Scarpa, M and Jordan, C and Gianni, A M
Journal: Human gene therapy (1999): 1875-84

A nonradioactive method for localization of endothelin receptor mRNA in situ.
Authors: McEwan, P E and Valdenaire, O and Sutherland, L and Webb, D J and Gray, G A
Journal: Journal of cardiovascular pharmacology (1998): S443-6

Focus luminescence assay: macroscopically visualized foci of human cytomegalovirus and varicella zoster virus infection.
Authors: Heider, H and Schroeder, C
Journal: Journal of virological methods (1997): 311-6

Histological and immunohistochemical diversities, and proliferative activity and grading in osteosarcomas.
Authors: Hasegawa, T and Hirose, T and Seki, K and Hizawa, K and Ishii, S and Wakabayashi, J
Journal: Cancer detection and prevention (1997): 280-7

Amplite 硝基还原酶检测试剂盒*生物发光法*

简要概述

产品基本信息

货号：12470

产品名称：Amplite 硝基还原酶检测试剂盒

规格：100 Tests

储存条件：-15℃避光防潮

保质期：12个月

 

试剂盒组分

组分A：NTR 基质(1瓶)

组分B：NTR反应缓冲液(1瓶)

组分C：NTR反应酶（1瓶）

组分D：NTR检测缓冲液（1瓶）

组分E：NTR检测混合物1（1瓶）

组分F：NTR检测混合物2（1瓶）

组分G：硝基还原酶标准品（1瓶）

组分H：DMSO（1瓶）

 

适用仪器

---

发光酶标仪
推荐孔板：	白色孔板

 

产品介绍

硝基还原酶（NTR）是一类参与含氮化合物还原的相关蛋白。它们在哺乳动物细胞中不存在，但在细菌中广泛存在。硝基还原酶在通过NAD（P）H依赖反应还原硝基芳香化合物中起着关键作用。NTR也是开发新型抗生素、降解污染物和癌症治疗的靶点。尽管NTR的定量在许多生物应用中变得极其重要，但目前大多数NTR检测都是基于低灵敏度和低吞吐量的ELISA实验。Amplite 硝基还原酶检测试剂盒为定量NTR活性提供了一种高灵敏度、简便的方法。该试剂盒使用一种荧光素衍生物，可被NTR荧光素选择性地还原。利用已知的荧光素酶检测系统可以方便地检测荧光素酶的生成量，它的发光强度与NTR活性成正比。该Amplite 硝基还原酶检测试剂盒具有最少的操作时间和稳定的发光信号。它能检测到低至20ng/mL的NTR。金畔生物是AAT Bioquest的中国代理商，为您提供最优质的Amplite 硝基还原酶检测试剂盒。

 

图示

 

图1.使用NOVO星型酶标仪（BMG Labtech），在白色96孔板中，用Amplite 发光型氮还原酶测定试剂盒，测定氮还原酶的剂量响应。 该试剂盒可检测低至20 ng / mL的NTR（数据是在添加NTR检测工作液后约5分钟获得的）。

 

iFluor 405琥珀酰亚胺酯

	货号	71501	存储条件	在零下15度以下保存, 避免光照
	规格	5 mg	价格	9180
	Ex (nm)	403	Em (nm)	427
	分子量	755.58	溶剂	DMSO
	产品详细介绍

简要概述

iFluor 405琥珀酰亚胺酯是iFluor系列荧光标记染料之一，可以覆盖整个可见光谱。所有iFluor 染料都具有优异的水溶性。它们的亲水性使有机溶剂的使用极小化。与常规的染料(如FITC，TRITC，Texas Red ，Cy3 ，Cy5和Cy7)相比，iFluor 染料具有更好的标记性能。一些iFluor 染料在某些抗体上明显优于Alexa Fluor 标记染料。它们是用于标记蛋白质和核酸的极便宜的荧光染料（替代Alexa Fluor 染料）。每种iFluor染料的开发都与特定的Alexa Fluor 或其他标记染料（如DyLight 染料）的光谱特性相匹配。

琥珀酰亚胺基（NHS）酯被证明是用于胺修饰的极佳试剂，因为形成的酰胺键基本上与天然肽键相同并且稳定。这些试剂通常是稳定的并且与脂族胺显示出良好的反应性和选择性。当琥珀酰亚胺酯化合物用于缀合反应时，需要考虑的因素很少：1.溶剂：在大多数情况下，活性染料应溶于无水二甲基甲酰胺（DMF）或二甲基亚砜（DMSO）中。 2.反应pH：胺与琥珀酰亚胺酯的标记反应强烈依赖于pH。胺反应性试剂与非质子化脂族胺基团反应，包括蛋白质的末端胺和赖氨酸的β-氨基。因此，胺酰化反应通常在pH 7.5以上进行。通过琥珀酰亚胺酯进行的蛋白质修饰通常可以在pH 8.5-9.5下进行。 3.反应缓冲液：使用胺反应试剂时，必须避免使用含有游离胺（如Tris和甘氨酸）和硫醇化合物的缓冲液。广泛用于蛋白质沉淀的铵盐（例如硫酸铵和乙酸铵）也必须在进行染料缀合之前除去（例如通过透析）。 4.反应温度：大多数缀合在室温下进行。特定标记反应可能需要升高或降低的温度。iFluor系列染料是AF系列染料的完美替代品。

点击查看光谱

点击查看操作方案

• AAT Bioquest iFluor 染料干货锦集 你想了解的都在这里
• 锦囊：iFluor 系列染料大集合

产品说明书

FITC–葡聚糖偶联物 (average MW = ~150K)

	货号	21704	存储条件	在零下15度以下保存, 避免光照
	规格	25 mg	价格	1836
	Ex (nm)	491	Em (nm)	516
	分子量	~150000	溶剂	Water
	产品详细介绍

简要概述

产品基本信息

货号：21704

产品名称：FITC–葡聚糖偶联物 

规格：25mg

储存条件：-15℃避光防潮

保质期：24个月

产品物理化学光谱特性

分子量：N/A

外观：固体

溶剂：水

激发波长(nm)：489

发射波长(nm)：515

产品介绍

FITC葡聚糖作为荧光示踪化合物用于心血管、微循环、灌注、细胞单层培养和细胞膜通透性的研究，支持血流、膜损伤、血管引流和肾脏清除等过程的测量。它还可用于利用微荧光法进行微循环和细胞通透性研究，并可用于动物的灌注研究。FITC葡聚糖也被用来研究植物细胞壁的孔隙率和毛细血管的渗透性。血浆蛋白已被证明不与FITC葡聚糖结合。小分子的FITC葡聚糖还常被用来测量细胞内吞和细胞连接通透性等过程。20kDa的FITC葡聚糖可用于研究细胞过程（如内吞作用和渗透作用）、内皮或上皮单层孔隙率、细胞层内的顶端基底外侧运动、细胞连接通透性和药物从结构（如水凝胶和胶原微棒）中的释放。金畔生物是AAT Bioquest的中国代理商，为您提供最优质的FITC–葡聚糖偶联物。 

点击查看光谱

参考文献

Lymphatic Reconnection and Restoration of Lymphatic Flow by Nonvascularized Lymph Node Transplantation: Real-Time Fluorescence Imaging Using Indocyanine Green and Fluorescein Isothiocyanate-Dextran
Authors: Maeda, T., Yamamoto, Y., Iwasaki, D., Hayashi, T., Funayama, E., Oyama, A., Murao, N., Furukawa, H.
Journal: Lymphat Res Biol (2018): 165-173

Fluorescein Isothiocyanate (FITC)-Dextran Extravasation as a Measure of Blood-Brain Barrier Permeability
Authors: Natarajan, R., Northrop, N., Yamamoto, B.
Journal: Curr Protoc Neurosci (2017): 9 58 1-9 58 15

Optimizing Fluorescein Isothiocyanate Dextran Measurement As a Biomarker in a 24-h Feed Restriction Model to Induce Gut Permeability in Broiler Chickens
Authors: Baxter, M. F. A., Merino-Guzman, R., Latorre, J. D., Mahaffey, B. D., Yang, Y., Teague, K. D., Graham, L. E., Wolfenden, A. D., Hern and ez-Velasco, X., Bielke, L. R., Hargis, B. M., Tellez, G.
Journal: Front Vet Sci (2017): 56

Adenosine receptor agonist NECA increases cerebral extravasation of fluorescein and low molecular weight dextran independent of blood-brain barrier modulation
Authors: Cheng, C. C., Yang, Y. L., Liao, K. H., Lai, T. W.
Journal: Sci Rep (2016): 23882

Analysis of hair follicle penetration of lidocaine and fluorescein isothiocyanate-dextran 4 kDa using hair follicle-plugging method
Authors: Horita, D., Yoshimoto, M., Todo, H., Sugibayashi, K.
Journal: Drug Dev Ind Pharm (2014): 345-51

Fluorescein-dextran sequestration in the reproductive tract of the migratory grasshopper Melanoplus sanguinipes (Orthoptera, Acridiidae)
Authors: Jones, N., Taub-Montemayor, T., Rankin, M. A.
Journal: Micron (2013): 80-4

Evaluation of changes in hepatic disposition of phenolsulfonphthalein, indocyanine green and fluorescein isothiocyanate-dextran at low temperatures using a rat liver perfusion system
Authors: Miyamoto, H., Miyake, H., Yoshikawa, N., Hirata, H., Ohwaki, Y., Fumoto, S., Sasaki, H., Nakamura, J., Nishida, K.
Journal: J Pharm Pharmacol (2012): 848-54

Effects of vitreous liquefaction on the intravitreal distribution of sodium fluorescein, fluorescein dextran, and fluorescent microparticles
Authors: Tan, L. E., Orilla, W., Hughes, P. M., Tsai, S., Burke, J. A., Wilson, C. G.
Journal: Invest Ophthalmol Vis Sci (2011): 1111-8

Lineage labeling of zebrafish cells with laser uncagable fluorescein dextran
Authors: Clanton, J. A., Shestopalov, I., Chen, J. K., Gamse, J. T.
Journal: J Vis Exp (2011): se name=”21700.enl” path=”C:UsersaatbiDropbox (AAT Bioquest)Catalogs & Website Working FilesProduct References21700.enl”>21700.enlEndNote8817Clanton, J. A.Shestopalov, I.Chen, J. K.Gamse, J. T.Department of Biological Sciences, Vanderbilt Univer

A method for assessment of blood volume parameters in pregnant sheep using fluorescein-labelled dextran
Authors: Rumball, C. W., Van Zijl, P., Rutl and M. D., Bloomfield, F. H., Harding, J. E.
Journal: Placenta (2008): 15-9