Fura-10,钾盐 货号21110-AAT Bioquest荧光染料

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品,欢迎访问AAT Bioquest荧光染料官网了解更多信息。

Fura-10,钾盐

Fura-10,钾盐

Fura-10,钾盐    货号21110 货号 21110 存储条件 在零下15度以下保存, 避免光照
规格 5×50 ug 价格 1200
Ex (nm) 354 Em (nm) 524
分子量 1024.27 溶剂 Water
产品详细介绍

简要概述

产品基本信息

货号:21110

产品名称:Fura-10,钾盐

规格:5×50 ug

储存条件:-15℃避光防潮

保质期:12个月

 

产品物理化学光谱特性

分子量:1024.27

溶剂:水

 

产品介绍

与Fluo-4等单波长探针不同,对于Ca2+,Fura探针的最大吸收(或荧光激发)从380 nm(Fura-2),415 nm(Fura-8和Fura-10)转变,与Ca2+结合的无螯合剂可达到约340 nm(Fura-2),355 nm(Fura-8和Fura-10),最大荧光发射的波长相对独立于Ca2+浓度。通过使用在340nm和380nm(对于Fura-2),355nm和415nm(对于Fura-8和Fura-10)进行激发并分配荧光强度来获得Ca2+依赖性荧光信号的最大动态范围在〜510 nm(Fura-2),525 nm(Fura-8和Fura-10)处检测到。根据该比率,可以使用从校准曲线得出的解离常数(Kd)估算细胞内Ca2+的水平。通过使用在两个波长下激发产生的荧光强度之比,诸如染料分布不均匀和光致漂白等因素将被最小化,因为它们应在相同程度上影响两种测量。校准溶液最初应不含重金属离子,例如锰,这可能会影响其荧光和对钙的亲和力。金畔生物是AAT Bioquest的中国代理商,为您提供最优质的Fura-10,钾盐。 

 

参考文献

A Novel Nicotinamide Adenine Dinucleotide Correction Method for Intracellular Ca2+ Measurement with Fura-2-Analog in Live Cells.
Authors: Lee, Jeong Hoon and Ha, Jeong Mi and Ho, Quynh Mai and Leem, Chae Hun
Journal: Journal of visualized experiments : JoVE (2019)

Calcium Imaging of Store-Operated Calcium (Ca2+) Entry (SOCE) in HEK293 Cells Using Fura-2.
Authors: Johnson, Martin
Journal: Methods in molecular biology (Clifton, N.J.) (2019): 163-172

Using FluoZin-3 and fura-2 to monitor acute accumulation of free intracellular Cd2+ in a pancreatic beta cell line.
Authors: Malaiyandi, Latha M and Sharthiya, Harsh and Barakat, Ameir N and Edwards, Joshua R and Dineley, Kirk E
Journal: Biometals : an international journal on the role of metal ions in biology, biochemistry, and medicine (2019): 951-964

A 340/380 nm light-emitting diode illuminator for Fura-2 AM ratiometric Ca2+ imaging of live cells with better than 5 nM precision.
Authors: Tinning, P W and Franssen, A J P M and Hridi, S U and Bushell, T J and McConnell, G
Journal: Journal of microscopy (2018): 212-220

Tuning the Spectroscopic Properties of Ratiometric Fluorescent Metal Indicators: Experimental and Computational Studies on Mag-fura-2 and Analogues.
Authors: Zhang, Guangqian and Jacquemin, Denis and Buccella, Daniela
Journal: The journal of physical chemistry. B (2017): 696-705

Heavy metal chelator TPEN attenuates fura-2 fluorescence changes induced by cadmium, mercury and methylmercury.
Authors: Ohkubo, Masato and Miyamoto, Atsushi and Shiraishi, Mitsuya
Journal: The Journal of veterinary medical science (2016): 761-7

Interferences of resveratrol with fura-2-derived fluorescence in intracellular free-Ca(2+) concentration determinations.
Authors: Santofimia-Castaño, Patricia and Salido, Gines M and Gonzalez, Antonio
Journal: Cytotechnology (2016): 1369-80

A Novel Nicotinamide Adenine Dinucleotide Correction Method for Mitochondrial Ca(2+) Measurement with FURA-2-FF in Single Permeabilized Ventricular Myocytes of Rat.
Authors: Lee, Jeong Hoon and Ha, Jeong Mi and Leem, Chae Hun
Journal: The Korean journal of physiology & pharmacology : official journal of the Korean Physiological Society and the Korean Society of Pharmacology (2015): 373-82

Resveratrol Interferes with Fura-2 Intracellular Calcium Measurements.
Authors: Kopp, Richard F and Leech, Colin A and Roe, Michael W
Journal: Journal of fluorescence (2014): 279-84

Resveratrol is not compatible with a Fura-2-based assay for measuring intracellular Ca²⁺ signaling.
Authors: Paudel, Ram Chandra and Kiviluoto, Santeri and Parys, Jan B and Bultynck, Geert
Journal: Biochemical and biophysical research communications (2014): 1626-30

说明书
Fura-10,钾盐.pdf

OTS-PEG-SVA 10k 对甲苯磺酰氧基聚乙二醇琥珀酰亚胺戊酸酯 分子量MW 10000

上海金畔生物科技有限公司提供OTS-PEG-SVA 10k 对甲苯磺酰氧基聚乙二醇琥珀酰亚胺戊酸酯 分子量MW 10000

品牌:
货号:JPB-07761
中文名:对甲苯磺酰氧基聚乙二醇琥珀酰亚胺戊酸酯 分子量MW 10000
英文名:OTS-PEG-SVA 10k
端基取代率:》95%
纯度:》95%
分子量:10k/10000
分散度:《1.05
包装:1克/5克/10克

更多产品,更多优惠,请联系我们!
订货热线:15221999938
qq号:2743691513
金畔博客:www.jinpanbio.cn
Email:sales@jinpanbio.com

NCO-PEG-VS 20k 异氰酸聚乙二醇乙烯砜 分子量MW 20000

上海金畔生物科技有限公司提供NCO-PEG-VS 20k 异氰酸聚乙二醇乙烯砜 分子量MW 20000

品牌:
货号:JPB-05853
中文名:异氰酸聚乙二醇乙烯砜 分子量MW 20000
英文名:NCO-PEG-VS 20k
端基取代率:》95%
纯度:》95%
分子量:20k/20000
分散度:《1.05
包装:1克/5克/10克

更多产品,更多优惠,请联系我们!
订货热线:15221999938
qq号:2743691513
金畔博客:www.jinpanbio.cn
Email:sales@jinpanbio.com

mPEG-OH 2k 甲氧基聚乙二醇羟基 分子量 2000

上海金畔生物科技有限公司提供mPEG-OH 2k 甲氧基聚乙二醇羟基 分子量 2000

品牌:JPB
货号:JPB-mPEG003
中文名:甲氧基聚乙二醇羟基 分子量 2000
英文名:mPEG-OH 2k
端基取代率:>95%
纯度:>95%
分子量:2k/2000
分散度:《1.05
包装:1克/5克/10克

更多产品,更多优惠,请联系我们!
上海金畔生物科技有限公司
订货热线:15221999938
qq号:2743691513
网 址: www.jinpanbio.com
金畔博客:www.jinpanbio.cn
Email:sales@jinpanbio.com

Fura-10,钾盐 货号21111-AAT Bioquest荧光染料

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品,欢迎访问AAT Bioquest荧光染料官网了解更多信息。

Fura-10,钾盐

Fura-10,钾盐

Fura-10,钾盐    货号21111 货号 21111 存储条件 在零下15度以下保存, 避免光照
规格 1 mg 价格 3732
Ex (nm) 354 Em (nm) 524
分子量 1024.27 溶剂 Water
产品详细介绍

简要概述

产品基本信息

货号:21110

产品名称:Fura-10,钾盐

规格:1mg

储存条件:-15℃避光防潮

保质期:12个月

 

产品物理化学光谱特性

分子量:1024.27

溶剂:水

 

产品介绍

与Fluo-4等单波长探针不同,对于Ca2+,Fura探针的最大吸收(或荧光激发)从380 nm(Fura-2),415 nm(Fura-8和Fura-10)转变,与Ca2+结合的无螯合剂可达到约340 nm(Fura-2),355 nm(Fura-8和Fura-10),最大荧光发射的波长相对独立于Ca2+浓度。通过使用在340nm和380nm(对于Fura-2),355nm和415nm(对于Fura-8和Fura-10)进行激发并分配荧光强度来获得Ca2+依赖性荧光信号的最大动态范围在〜510 nm(Fura-2),525 nm(Fura-8和Fura-10)处检测到。根据该比率,可以使用从校准曲线得出的解离常数(Kd)估算细胞内Ca2+的水平。通过使用在两个波长下激发产生的荧光强度之比,诸如染料分布不均匀和光致漂白等因素将被最小化,因为它们应在相同程度上影响两种测量。校准溶液最初应不含重金属离子,例如锰,这可能会影响其荧光和对钙的亲和力。金畔生物是AAT Bioquest的中国代理商,为您提供最优质的Fura-10,钾盐。 

 

参考文献

A Novel Nicotinamide Adenine Dinucleotide Correction Method for Intracellular Ca2+ Measurement with Fura-2-Analog in Live Cells.
Authors: Lee, Jeong Hoon and Ha, Jeong Mi and Ho, Quynh Mai and Leem, Chae Hun
Journal: Journal of visualized experiments : JoVE (2019)

Calcium Imaging of Store-Operated Calcium (Ca2+) Entry (SOCE) in HEK293 Cells Using Fura-2.
Authors: Johnson, Martin
Journal: Methods in molecular biology (Clifton, N.J.) (2019): 163-172

Using FluoZin-3 and fura-2 to monitor acute accumulation of free intracellular Cd2+ in a pancreatic beta cell line.
Authors: Malaiyandi, Latha M and Sharthiya, Harsh and Barakat, Ameir N and Edwards, Joshua R and Dineley, Kirk E
Journal: Biometals : an international journal on the role of metal ions in biology, biochemistry, and medicine (2019): 951-964

A 340/380 nm light-emitting diode illuminator for Fura-2 AM ratiometric Ca2+ imaging of live cells with better than 5 nM precision.
Authors: Tinning, P W and Franssen, A J P M and Hridi, S U and Bushell, T J and McConnell, G
Journal: Journal of microscopy (2018): 212-220

Tuning the Spectroscopic Properties of Ratiometric Fluorescent Metal Indicators: Experimental and Computational Studies on Mag-fura-2 and Analogues.
Authors: Zhang, Guangqian and Jacquemin, Denis and Buccella, Daniela
Journal: The journal of physical chemistry. B (2017): 696-705

Heavy metal chelator TPEN attenuates fura-2 fluorescence changes induced by cadmium, mercury and methylmercury.
Authors: Ohkubo, Masato and Miyamoto, Atsushi and Shiraishi, Mitsuya
Journal: The Journal of veterinary medical science (2016): 761-7

Interferences of resveratrol with fura-2-derived fluorescence in intracellular free-Ca(2+) concentration determinations.
Authors: Santofimia-Castaño, Patricia and Salido, Gines M and Gonzalez, Antonio
Journal: Cytotechnology (2016): 1369-80

A Novel Nicotinamide Adenine Dinucleotide Correction Method for Mitochondrial Ca(2+) Measurement with FURA-2-FF in Single Permeabilized Ventricular Myocytes of Rat.
Authors: Lee, Jeong Hoon and Ha, Jeong Mi and Leem, Chae Hun
Journal: The Korean journal of physiology & pharmacology : official journal of the Korean Physiological Society and the Korean Society of Pharmacology (2015): 373-82

Resveratrol Interferes with Fura-2 Intracellular Calcium Measurements.
Authors: Kopp, Richard F and Leech, Colin A and Roe, Michael W
Journal: Journal of fluorescence (2014): 279-84

Resveratrol is not compatible with a Fura-2-based assay for measuring intracellular Ca²⁺ signaling.
Authors: Paudel, Ram Chandra and Kiviluoto, Santeri and Parys, Jan B and Bultynck, Geert
Journal: Biochemical and biophysical research communications (2014): 1626-30

说明书
Fura-10,钾盐.pdf

ALD-PEG-FITC 2k 丙醛聚乙二醇荧光素 分子量MW 2000

上海金畔生物科技有限公司提供ALD-PEG-FITC 2k 丙醛聚乙二醇荧光素 分子量MW 2000

品牌:
货号:JPB-03714
中文名:丙醛聚乙二醇荧光素 分子量MW 2000
英文名:ALD-PEG-FITC 2k
端基取代率:》95%
纯度:》95%
分子量:2k/2000
分散度:《1.05
包装:1克/5克/10克

更多产品,更多优惠,请联系我们!
订货热线:15221999938
qq号:2743691513
金畔博客:www.jinpanbio.cn
Email:sales@jinpanbio.com

淬灭剂DABCYL-DBCO 货号2010-AAT Bioquest荧光染料

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品,欢迎访问AAT Bioquest荧光染料官网了解更多信息。

淬灭剂DABCYL-DBCO

淬灭剂DABCYL-DBCO

淬灭剂DABCYL-DBCO    货号2010 货号 2010 存储条件 在零下15度以下保存, 避免光照
规格 5 mg 价格 3732
Ex (nm) Em (nm)
分子量 527.63 溶剂 DMSO
产品详细介绍

简要概述

产品基本信息

货号:2010

产品名称:淬灭剂DABCYL-DBCO

规格:5mg

储存条件:-15℃避光防潮

保质期:12个月

 

产品物理化学光谱特性

分子量:527.63

溶剂:DMSO

 

产品介绍

DABCYL是用于制备各种基于FRET的核酸探针和蛋白酶底物的最常见的淬灭剂之一。它是一种吸收来自荧光团的激发能并将其作为热量消散的物质。当它和荧光团紧密靠近时,例如在分子或蛋白质中,荧光团的发射受到抑制。此效应可用于研究分子的几何形状和运动。DABCYL-DBCO是通过基于DBCO的点击化学开发FRET探针,该化学是最著名的应变促进叠氮化物-炔烃环加成反应(SPAAC)之一。金畔生物是AAT Bioquest的中国代理商,为您提供最优质的淬灭剂DABCYL-DBCO。

 

参考文献

A Cell Membrane-Targeting Self-Delivery Chimeric Peptide for Enhanced Photodynamic Therapy and In Situ Therapeutic Feedback.
Authors: Ma, Wen and Sha, Sui-Nan and Chen, Pei-Ling and Yu, Meng and Chen, Jian-Jun and Huang, Chao-Bo and Yu, Bin and Liu, Yun and Liu, Li-Han and Yu, Zhi-Qiang
Journal: Advanced healthcare materials (2020): e1901100

Array-based in situ fluorescence assay for profiling multiplex matrix metalloproteinases activities in tissue section.
Authors: Lei, Zhen and Jian, Minghong and Wei, Jia and Wang, Yaoqi and Meng, Xianying and Wang, Zhenxin
Journal: Analytica chimica acta (2019): 112-118

FRET-Based Assays to Determine Calpain Activity.
Authors: McCartney, Christian-Scott E and Davies, Peter L
Journal: Methods in molecular biology (Clifton, N.J.) (2019): 39-55

Polymeric fluorescent heparin as one-step FRET substrate of human heparanase.
Authors: Sistla, Jyothi C and Morla, Shravan and Alabbas, Al-Humaidi B and Kalathur, Ravi C and Sharon, Chetna and Patel, Bhaumik B and Desai, Umesh R
Journal: Carbohydrate polymers (2019): 385-391

The conversion of azo-quenchers to fluorophores.
Authors: Hofstetter, O and Hofstetter, H and Miron, T and Wilchek, M
Journal: Analytical biochemistry (2019): 113400

A FRET-based dual-color evanescent wave optical fiber aptasensor for simultaneous fluorometric determination of aflatoxin M1 and ochratoxin A.
Authors: Song, Dan and Yang, Rong and Fang, Shunyan and Liu, Yanping and Long, Feng
Journal: Mikrochimica acta (2018): 508

Development of a peptide substrate for detection of sunn pest damage in wheat flour.
Authors: Hançerlioğulları, Begüm Zeynep and Köksel, Hamit and Dudak, Fahriye Ceyda
Journal: Journal of the science of food and agriculture (2018): 5677-5682

A dual-colored ratiometric-fluorescent oligonucleotide probe for the detection of human telomerase RNA in cell extracts.
Authors: Ning, Dianhua and He, Changtian and Liu, Zhengjie and Liu, Cui and Wu, Qilong and Zhao, TingTing and Liu, Renyong
Journal: The Analyst (2017): 1697-1702

Chemoenzymatic synthesis of fluorogenic phospholipids and evaluation in assays of phospholipases A, C and D.
Authors: Piel, Mathilde S and Peters, Günther H J and Brask, Jesper
Journal: Chemistry and physics of lipids (2017): 49-54

Detection of human neutrophil elastase (HNE) on wound dressings as marker of inflammation.
Authors: Ferreira, Ana V and Perelshtein, Ilana and Perkas, Nina and Gedanken, Aharon and Cunha, Joana and Cavaco-Paulo, Artur
Journal: Applied microbiology and biotechnology (2017): 1443-1454

说明书
淬灭剂DABCYL-DBCO.pdf

OTS-PEG-Thiol 40k 对甲苯磺酰氧基聚乙二醇巯基 分子量MW 40000

上海金畔生物科技有限公司提供OTS-PEG-Thiol 40k 对甲苯磺酰氧基聚乙二醇巯基 分子量MW 40000

品牌:
货号:JPB-07841
中文名:对甲苯磺酰氧基聚乙二醇巯基 分子量MW 40000
英文名:OTS-PEG-Thiol 40k
端基取代率:》95%
纯度:》95%
分子量:40k/40000
分散度:《1.05
包装:1克/5克/10克

更多产品,更多优惠,请联系我们!
订货热线:15221999938
qq号:2743691513
金畔博客:www.jinpanbio.cn
Email:sales@jinpanbio.com