钙黄绿素深红

	货号	21902	存储条件	在零下15度以下保存, 避免光照
	规格	1 mg	价格	2604
	Ex (nm)	643	Em (nm)	663
	分子量	373.66	溶剂	DMSO
	产品详细介绍

简要概述

产品基本信息

货号：21902

产品名称：钙黄绿素深红

规格：1mg

储存条件：-15℃避光防潮

保质期：24个月

产品物理化学光谱特性

分子量：373.66

溶剂：DMSO

激发波长(nm)：646

发射波长(nm)：659

产品介绍

钙黄绿素深红是美国AAT Bioquest生产的钙指示剂，钙黄绿素AM是最流行的荧光探针之一，用于标记和监测活细胞的细胞功能。但是，钙黄绿素AM的单色使得无法在多色应用中使用这种有价值的试剂。例如，由于与GFP相同的颜色，不可能在GFP转染的细胞中使用钙黄绿素AM。为了解决钙黄绿素AM的颜色限制，我们开发了钙黄绿橙等一系列产品。这些新的Calcein AM类似物与Calcein AM结合使用，可以对活细胞进行多色标记和功能分析。钙黄绿素深红TM是钙黄绿素深红 乙酸酯在细胞中的酯酶水解产物。钙黄绿素Deep Red 染料可以使用通用的Cy5滤波片进行检测。它用作Calcein Deep Red 乙酸酯的荧光参考标准。金畔生物是AAT Bioquest的中国代理商，为您提供最优质的钙黄绿素深红。 

点击查看光谱

点击查看实验方案

参考文献

Functional imaging of neuronal activity of auditory cortex by using Cal-520 in anesthetized and awake mice
Authors: Jingcheng Li, Jianxiong Zhang, Meng Wang, Junxia Pan, Xiaowei Chen, Xiang Liao
Journal: Biomedical Optics Express (2017): 2599–2610

NINJ2–A novel regulator of endothelial inflammation and activation
Authors: Jingjing Wang, Jingjing Fa, Pengyun Wang, Xinzhen Jia, Huixin Peng, Jing Chen, Yifan Wang, Chenhui Wang, Qiuyun Chen, Xin Tu
Journal: Cellular Signalling (2017)

Erythropoietin Stimulates Endothelial Progenitor Cells to Induce Endothelialization in an Aneurysm Neck After Coil Embolization by Modulating Vascular Endothelial Growth Factor
Authors: Peixi Liu, Yingjie Zhou, Qingzhu An, Yaying Song, Xi Chen, Guo-Yuan Yang, Wei Zhu
Journal: MEDICINE (2016): 1–8

Flexible Endoscopic Spray Application of Respiratory Epithelial Cells as Platform Technology to Apply Cells in Tubular Organs
Authors: Anja Lena Thiebes, Manuel Armin Reddemann, Johannes Palmer, Reinhold Kneer, Stefan Jockenhoevel, Christian Gabriel Cornelissen
Journal: Tissue Engineering Part C: Methods (2016): 322–331

Influence of hypothermia and subsequent rewarming upon leukocyte-endothelial interactions and expression of Junctional-Adhesion-Molecules A and B
Authors: Nicolai V Bogert, Isabella Werner, Angela Kornberger, Patrick Meybohm, Anton Moritz, Till Keller, Ulrich A Stock, Andres Beiras-Fernandez
Journal: Scientific reports (2016)

Inhibition of ABC transport proteins by oil sands process affected water
Authors: Hattan A Alharbi, David MV Saunders, Ahmed Al-Mousa, Jane Alcorn, Alberto S Pereira, Jonathan W Martin, John P Giesy, Steve B Wiseman
Journal: Aquatic Toxicology (2016): 81–88

Rapid generation of collagen-based microtissues to study cell–matrix interactions
Authors: Marie-Elena Brett, Alexandra L Crampton, David K Wood
Journal: Technology (2016): 1–8

Toxicokinetics and toxicodynamics of chlorpyrifos is altered in embryos of Japanese medaka exposed to oil sands process-affected water: evidence for inhibition of P-glycoprotein
Authors: Hattan A Alharbi, Jane Alcorn, Ahmed Al-Mousa, John P Giesy, Steve B Wiseman
Journal: Journal of Applied Toxicology (2016)

Spraying respiratory epithelial cells to coat tissue-engineered constructs
Authors: Anja Lena Thiebes, Stefanie Albers, Christian Klopsch, Stefan Jockenhoevel, Christian G Cornelissen
Journal: BioResearch open access (2015): 278–287

The efficient hemostatic effect of Antarctic krill chitosan is related to its hydration property
Authors: Shuai Wu, Zhuoyao Huang, Jianhui Yue, Di Liu, Ting Wang, Pierre Ezanno, Changshun Ruan, Xiaoli Zhao, William W Lu, Haobo Pan
Journal: Carbohydrate polymers (2015): 295–303

相关产品

钙黄绿素蓝，钠盐

	货号	21909	存储条件	在零下15度以下保存, 避免光照
	规格	1 mg	价格	2604
	Ex (nm)	359	Em (nm)	458
	分子量	566.35	溶剂	DMSO
	产品详细介绍

简要概述

产品基本信息

货号：21909

产品名称：钙黄绿素蓝，钠盐

规格：1mg

储存条件：-15℃避光防潮

保质期：24个月

产品物理化学光谱特性

分子量：566.35

溶剂：DMSO

激发波长(nm)：360

发射波长(nm)：445

产品介绍

钙黄绿素蓝，钠盐是美国AAT Bioquest生产的钙指示剂，钙黄绿素UltraBlue 具有与钙黄绿蓝相似的光谱特性。但是，与钙黄绿素蓝相比，钙黄绿素UltraBlue 在生理pH下具有更高的光稳定性和更强的荧光强度，使其比钙黄绿素蓝更耐用。金畔生物是AAT Bioquest的中国代理商，为您提供最优质的钙黄绿素蓝，钠盐。 

参考文献

Functional imaging of neuronal activity of auditory cortex by using Cal-520 in anesthetized and awake mice
Authors: Jingcheng Li, Jianxiong Zhang, Meng Wang, Junxia Pan, Xiaowei Chen, Xiang Liao
Journal: Biomedical Optics Express (2017): 2599–2610

NINJ2–A novel regulator of endothelial inflammation and activation
Authors: Jingjing Wang, Jingjing Fa, Pengyun Wang, Xinzhen Jia, Huixin Peng, Jing Chen, Yifan Wang, Chenhui Wang, Qiuyun Chen, Xin Tu
Journal: Cellular Signalling (2017)

Erythropoietin Stimulates Endothelial Progenitor Cells to Induce Endothelialization in an Aneurysm Neck After Coil Embolization by Modulating Vascular Endothelial Growth Factor
Authors: Peixi Liu, Yingjie Zhou, Qingzhu An, Yaying Song, Xi Chen, Guo-Yuan Yang, Wei Zhu
Journal: MEDICINE (2016): 1–8

Flexible Endoscopic Spray Application of Respiratory Epithelial Cells as Platform Technology to Apply Cells in Tubular Organs
Authors: Anja Lena Thiebes, Manuel Armin Reddemann, Johannes Palmer, Reinhold Kneer, Stefan Jockenhoevel, Christian Gabriel Cornelissen
Journal: Tissue Engineering Part C: Methods (2016): 322–331

Influence of hypothermia and subsequent rewarming upon leukocyte-endothelial interactions and expression of Junctional-Adhesion-Molecules A and B
Authors: Nicolai V Bogert, Isabella Werner, Angela Kornberger, Patrick Meybohm, Anton Moritz, Till Keller, Ulrich A Stock, Andres Beiras-Fernandez
Journal: Scientific reports (2016)

Inhibition of ABC transport proteins by oil sands process affected water
Authors: Hattan A Alharbi, David MV Saunders, Ahmed Al-Mousa, Jane Alcorn, Alberto S Pereira, Jonathan W Martin, John P Giesy, Steve B Wiseman
Journal: Aquatic Toxicology (2016): 81–88

Rapid generation of collagen-based microtissues to study cell–matrix interactions
Authors: Marie-Elena Brett, Alexandra L Crampton, David K Wood
Journal: Technology (2016): 1–8

Toxicokinetics and toxicodynamics of chlorpyrifos is altered in embryos of Japanese medaka exposed to oil sands process-affected water: evidence for inhibition of P-glycoprotein
Authors: Hattan A Alharbi, Jane Alcorn, Ahmed Al-Mousa, John P Giesy, Steve B Wiseman
Journal: Journal of Applied Toxicology (2016)

Spraying respiratory epithelial cells to coat tissue-engineered constructs
Authors: Anja Lena Thiebes, Stefanie Albers, Christian Klopsch, Stefan Jockenhoevel, Christian G Cornelissen
Journal: BioResearch open access (2015): 278–287

The efficient hemostatic effect of Antarctic krill chitosan is related to its hydration property
Authors: Shuai Wu, Zhuoyao Huang, Jianhui Yue, Di Liu, Ting Wang, Pierre Ezanno, Changshun Ruan, Xiaoli Zhao, William W Lu, Haobo Pan
Journal: Carbohydrate polymers (2015): 295–303

相关产品

钙橙素双乙酸盐

	货号	22009	存储条件	在零下15度以下保存, 避免光照
	规格	1 mg	价格	2604
	Ex (nm)	531	Em (nm)	545
	分子量	877.24	溶剂	DMSO
	产品详细介绍

简要概述

产品基本信息

货号：22009

产品名称：钙橙素双乙酸盐

规格：1mg

储存条件：-15℃避光防潮

保质期：24个月

产品物理化学光谱特性

分子量：877.24

溶剂：DMSO

激发波长(nm)：525

发射波长(nm)：550

产品介绍

钙橙素双乙酸盐是美国AAT Bioquest生产的细胞活力测定试剂，钙黄绿素AM是最流行的荧光探针之一，用于标记和监测活细胞的细胞功能。但是，钙黄绿素AM的单色使得无法在多色应用中使用这种有价值的试剂。例如，由于与GFP相同的颜色，不可能在结合GFP的细胞中使用钙黄绿素AM。为了解决钙黄绿素AM的颜色限制，我们开发了钙黄绿橙 ，钙黄绿 和钙黄绿 。这些新的Calcein AM类似物与Calcein AM结合使用，可以对活细胞进行多色标记和功能分析。非荧光钙黄绿橙 二醋酸酯可轻松进入活细胞并水解产生强荧光钙黄绿橙 染料。AAT Bioquest还提供Calcein Orange 作为Calcein Orange diacetate的参考染料。金畔生物是AAT Bioquest的中国代理商，为您提供最优质的钙橙素双乙酸盐。 

点击查看光谱

点击查看实验方案

参考文献

活性氧 Cell Meter 荧光法胞内总ROS检测试剂盒 绿色荧光

	货号	22900	存储条件	在零下15度以下保存, 避免光照
	规格	200 Tests	价格	2604
	Ex (nm)	492	Em (nm)	520
	分子量		溶剂
	产品详细介绍

简要概述

Cell Meter 荧光法胞内总ROS检测试剂盒是美国AAT Bioquest生产的用于ROS检测的试剂盒，Cell Meter检测试剂盒是一类用于检测细胞功能的系列工具，包括细胞活性、细胞毒性、细胞凋亡、细胞膜电位以及细胞周期等方面的指标。每种检测方案均能提供不同荧光颜色的检测方案。这些高效的检测方案为从多角度研究细胞功能活动提供了一种十分有效的方法。活性氧分子（ROS）是氧正常代谢过程的固有副产品，在细胞信号及转导过程中扮演着重要的角色。然而，在氧化应激阶段，ROS水平显著增加。ROS的积聚导致细胞结构的重大破坏。氧化应激通常在心血管疾病，糖尿病，骨质疏松，中风，炎症以及许多神经变性疾病和癌症中被被发现。ROS的测定能帮助我们理解氧化应激如何调节改变的胞内途径。Amplite ROS荧光法检测试剂盒使用我们独特的ROS指示器在活细胞中测定ROS。Amplite ROS Green是细胞通透的，当它与ROS反应时能产生绿色的荧光。本试剂盒具备优化的即用配制，能与高通量筛选的液体控制设备相配。Amplite ROS荧光法检测试剂盒能提供敏感的、一步荧光实验来检测活细胞中胞内的ROS，仅需一小时的孵育。本实验能在96孔或者384孔微孔板中进行，可以实现自动化无需分离步骤。它的信号能轻松被荧光酶标仪或者荧光显微镜读取。金畔生物是AAT Bioquest的中国代理商，为您提供最优质的Cell Meter 荧光法胞内总ROS检测试剂盒。 

活性氧（ROS）篇：包含总ROS和多种活性氧离子检测试剂大全

产品说明书

样品实验方案

简要概述

1.在生长培养基中制备细胞
2.添加Amplite ROS绿色工作溶液（96孔板100μL/孔或384孔板25μL/孔）
3.将细胞在37℃下染色60分钟
4.用测试化合物处理细胞以诱导ROS
5.在Ex / Em = 490/525 nm（截止= 515 nm）或带有FITC滤光片组的荧光显微镜下监测荧光增加（底部读取模式）

溶液配制

1.储备溶液配制

除非另有说明，否则所有未使用的储备溶液应分成一次性等分试样，并在制备后储存在-20°C。 避免反复冻融循环。
1. 1Amplite ROS Green原液（500X）：
将40μLDMSO（组分C）加入到Amplite ROS Green（组分A）的小瓶中并充分混合以制备500X Amplite  ROS Green原液。 避光。 注意：20μL的500X Amplite ROS Green原液足以容纳1个平板。 

2.制备工作溶液

将20μL500XAmplite ROS Green储备溶液加入10 mL分析缓冲液（组分B）中，充分混合，制成Amplite ROS Green工作溶液。 注意：此Amplite ROS Green工作溶液在室温下稳定至少2小时。有关细胞样品制备的指南，请点击查看。

操作步骤

1.将100μL/孔（96孔板）或25μL/孔（384孔板）的Amplite ROS Green工作溶液加入细胞板中。

2.将细胞在5％CO₂,37℃培养箱中孵育1小时。

3.用所需缓冲液（如PBS或HHBS）中的20μL11X测试化合物（96孔板）或10μL6X测试化合物（384孔板）处理细胞。 对于对照孔（未处理的细胞），加入相应量的化合物缓冲液。

4.为了诱导ROS，将细胞板在室温下或在5％CO₂,37℃培养箱中孵育至少15分钟或所需的一段时间（对于用1mM H₂O₂处理的Hela细胞30分钟）。

5.使用荧光酶标仪（底部读取模式）在Ex / Em = 490 / 525nm（截止= 515nm）监测荧光增加，或使用具有FITC滤光器组的荧光显微镜观察细胞。

数据分析

参考文献

Tyrosine kinase inhibitor conjugated quantum dots for non-small cell lung cancer (NSCLC) treatment
Authors: Nishant S Kulkarni, Vineela Parvathaneni, Snehal K Shukla, Leonard Barasa, Jeanette C Perron, Sabesan Yoganathan, Aaron Muth, Vivek Gupta
Journal: European Journal of Pharmaceutical Sciences (2019)

Activation of p53-Mediated Apoptosis Pathway in HSC3 Cancer Cell Irradiated by Atmospheric DBD Oxygen Plasma
Authors: Nobuya Hayashi, Yukie Miyamaru, Reona Aijima, Yoshio Yamashita
Journal: IEEE Transactions on Plasma Science (2018): 1–7

Role of GPx3 in PPARγ-induced protection against COPD-associated oxidative stress
Authors: Aravind T Reddy, Sowmya P Lakshmi, Asoka Banno, Raju C Reddy
Journal: Free Radical Biology and Medicine (2018)

Topiramate exhibits anti-tumorigenic and metastatic effects in ovarian cancer cells
Authors: Guangxu Xu, Ziwei Fang, Leslie H Clark, Wenchuan Sun, Yajie Yin, Rong Zhang, Stephanie A Sullivan, Arthur-Quan Tran, Weimin Kong, Jiandong Wang
Journal: Am J Transl Res (2018): 1663–1676

Anti-proliferation effect of blue light-emitting diodes against antibiotic-resistant Helicobacter pylori
Authors: Jianwei Ma, Takahiro Hiratsuka, Tsuyoshi Etoh, Junko Akada, Hajime Fujishima, Norio Shiraishi, Yoshio Yamaoka, Masafumi Inomata
Journal: Journal of Gastroenterology and Hepatology (2017)

Notoginsenoside R1 attenuates high glucose-induced endothelial damage in rat retinal capillary endothelial cells by modulating the intracellular redox state
Authors: Chunlan Fan, Yuan Qiao, Minke Tang
Journal: Drug Design, Development and Therapy (2017): 3343

Good hydration and cell-biological performances of superparamagnetic calcium phosphate cement with concentration-dependent osteogenesis and angiogenesis induced by ferric iron
Authors: J Zhang, HS Shi, JQ Liu, T Yu, ZH Shen, JD Ye
Journal: Journal of Materials Chemistry B (2015): 8782–8795

Topiramate Protects Pericytes from Glucotoxicity: Role for Mitochondrial CA VA in Cerebromicrovascular Disease in Diabetes
Authors: Ping Patrick, Tulin O Price, Ana L Diogo, Nader Sheibani, William A Banks, Gul N Shah
Journal: Journal of endocrinology and diabetes (2015)

Down-regulated peroxisome proliferator-activated receptor γ (PPARγ) in lung epithelial cells promotes a PPARγ agonist-reversible proinflammatory phenotype in chronic obstructive pulmonary disease (COPD)
Authors: Sowmya P Lakshmi, Aravind T Reddy, Yingze Zhang, Frank C Sciurba, Rama K Mallampalli, Steven R Duncan, Raju C Reddy
Journal: Journal of Biological Chemistry (2014): 6383–6393

Superoxide dismutase as a target of clioquinol-induced neurotoxicity
Authors: Kazuyuki Kawamura, Yukiko Kuroda, Masako Sogo, Miki Fujimoto, Toshio Inui, Takao Mitsui
Journal: Biochemical and biophysical research communications (2014): 181–185

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