Cell Meter 线粒体自噬荧光成像试剂盒*红色荧光*

	货号	22998	存储条件	Multiple
	规格	100 Tests	价格	3924
	Ex (nm)		Em (nm)
	分子量		溶剂
	产品详细介绍

简要概述

产品基本信息

货号：22998

产品名称：Cell Meter 线粒体自噬荧光成像试剂盒

规格：100 Tests

储存条件：-15℃避光防潮

保质期：24个月

 

试剂盒成分

组分A：Mitophagy Red 1小瓶
组分B：染色缓冲液1瓶（50毫升）
组分C：DMSO 1小瓶（50 µL）

 

适用仪器

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荧光显微镜
激发：	Cy3/TRITC滤波片组
发射：	Cy3/TRITC滤波片组
推荐孔板：	黑色透明
滤波片：	Cy3/TRITC滤波片组

 

产品介绍

线粒体自噬（也称为“有丝分裂”）似乎与阿尔茨海默病和帕金森病有关，主要是由去极化线粒体的积累引起的。有丝分裂是一个清除系统，它清除由氧化应激和DNA损伤引起的功能失调的线粒体，使其与自身噬菌体隔离，然后与溶酶体融合并被降解。Cell Meter 线粒体自噬荧光成像试剂盒使用Mitophagy Red 作为有丝分裂探针，它能使线粒体在多种哺乳动物细胞类型上迅速而均匀地染色，并在有丝分裂诱导时转移到溶酶体。Cell Meter 线粒体自噬荧光成像试剂盒提供了一种可以检测线粒体的优秀的方法，可作为悬浮或附着活细胞有丝分裂吞噬的指标。Mitophagy Red在活细胞中是足够稳定的，它提供了许多时间来研究大多数活细胞的动力学，实验条件与细胞培养基相适应。Mitophagy Red的激发/发射光线非常适合使用Cy3/TRITC滤波器组，可以很容易地与GFP表达细胞系结合。金畔生物是AAT Bioquest的中国代理商，为您提供最优质的Cell Meter 线粒体自噬荧光成像试剂盒。 

 

图示

 

图1. 在96孔板中，与Mitophagy Red 和Hoechst 33342（Cat＃17535）共染色的HeLa细胞的荧光图像。 在添加CCCP（10 uM）1分钟之前（左侧）和之后（右侧）获取图像。 使用带有Cy3 / TRITC滤光片的荧光显微镜对细胞成像。

图2. HeLa细胞与Mitophagy Red 和MitoLite Green FM（Cat＃22695）共染色的荧光图像，位于96孔板中。 在添加CCCP（10 uM）1分钟之前（左侧）和之后（右侧）获取图像。 使用荧光显微镜对细胞进行成像，荧光显微镜具有用于Mitophagy Red 的Cy3 / TRITC滤波片和用于MitoLite Green FM的FITC滤波片，并且彼此重叠。

 

Texas Red TCO*单一异构体*

	货号	899	存储条件	在零下15度以下保存, 避免光照
	规格	1 mg	价格	2472
	Ex (nm)	586	Em (nm)	603
	分子量	815.01	溶剂	DMSO
	产品详细介绍

简要概述

产品基本信息

货号：899

产品名称：Texas Red TCO*单一异构体*

规格：1 mg

储存条件：-15℃避光防潮

保质期：12个月

产品物理化学光谱特性

分子量：815.01

外观：固体

溶剂：DMSO

Ex(nm):586

Em(nm):603

产品介绍

四嗪-反式-环辛烯（TCO）的连接构成了快速无毒的生物分子标记方法。四嗪功能化的分子与TCO功能化的分子反应，通过二氢吡嗪部分形成稳定的结合物。这种反电子需求环加成反应由于使用TCO作为亲二烯体而具有极快的环加成动力学的潜力而广受欢迎。AAT Bioquest提供了一组含四嗪和TCO的染料，用于探索可以利用这种强大点击反应的各种生物系统。Texas Red-TCO可以很容易地用于标记四嗪修饰的生物分子，用于荧光成像和其他基于荧光的生化分析。它广泛用于标记四嗪修饰的肽，蛋白质和寡核苷酸等。

点击查看光谱

参考文献

A Pretargeted Imaging Strategy for Immune Checkpoint Ligand PD-L1 Expression in Tumor Based on Bioorthogonal Diels-Alder Click Chemistry.
Authors: Qiu, Lin and Tan, Hui and Lin, Qingyu and Si, Zhan and Mao, Wujian and Wang, Tingting and Fu, Zhequan and Cheng, Dengfeng and Shi, Hongcheng
Journal: Molecular imaging and biology (2020): 842-853

A Systematic Study of Coumarin-Tetrazine Light-Up Probes for Bioorthogonal Fluorescence Imaging.
Authors: Galeta, Juraj and Dzijak, Rastislav and Obořil, Jan and Dračínský, Martin and Vrabel, Milan
Journal: Chemistry (Weinheim an der Bergstrasse, Germany) (2020)

Antibody Pretargeting Based on Bioorthogonal Click Chemistry for Cancer Imaging and Targeted Radionuclide Therapy.
Authors: Rondon, Aurélie and Degoul, Françoise
Journal: Bioconjugate chemistry (2020): 159-173

Cellular Delivery of Bioorthogonal Pretargeting Therapeutics in PSMA-Positive Prostate Cancer.
Authors: Hapuarachchige, Sudath and Huang, Colin T and Donnelly, Madeline C and Bařinka, Cyril and Lupold, Shawn E and Pomper, Martin G and Artemov, Dmitri
Journal: Molecular pharmaceutics (2020): 98-108

Conditionally Controlling Human TLR2 Activity via Trans-Cyclooctene Caged Ligands.
Authors: van de Graaff, Michel J and Oosenbrug, Timo and Marqvorsen, Mikkel H S and Nascimento, Clarissa R and de Geus, Mark A R and Manoury, Bénédicte and Ressing, Maaike E and van Kasteren, Sander I
Journal: Bioconjugate chemistry (2020): 1685-1692

Effect of Modulating FcRn Binding on Direct and Pretargeted Tumor Uptake of Full-length Antibodies.
Authors: Nazarova, Lidia and Rafidi, Hanine and Mandikian, Danielle and Ferl, Gregory Z and Koerber, James T and Davies, Christopher W and Ulufatu, Sheila and Ho, Jason and Lau, Jeffrey and Yu, Shang-Fan and Ernst, James and Sadowsky, Jack D and Boswell, C Andrew
Journal: Molecular cancer therapeutics (2020): 1052-1058

Fluorogenic Bifunctional trans-Cyclooctenes as Efficient Tools for Investigating Click-to-Release Kinetics.
Authors: de Geus, Mark A R and Maurits, Elmer and Sarris, Alexi J C and Hansen, Thomas and Kloet, Max S and Kamphorst, Kiki and Ten Hoeve, Wolter and Robillard, Marc S and Pannwitz, Andrea and Bonnet, Sylvestre A and Codée, Jeroen D C and Filippov, Dmitri V and Overkleeft, Herman S and van Kasteren, Sander I
Journal: Chemistry (Weinheim an der Bergstrasse, Germany) (2020)

Hybrid Imaging Agents for Pretargeting Applications Based on Fusarinine C-Proof of Concept.
Authors: Summer, Dominik and Petrik, Milos and Mayr, Sonja and Hermann, Martin and Kaeopookum, Piriya and Pfister, Joachim and Klingler, Maximilian and Rangger, Christine and Haas, Hubertus and Decristoforo, Clemens
Journal: Molecules (Basel, Switzerland) (2020)

Injectable Click Chemistry-based Bioadhesives for Accelerated Wound Closure.
Authors: Li, Shuxin and Zhou, Jun and Huang, YiHui and Roy, Joyita and Zhou, Ning and Yum, Kyungsuk and Sun, Xiankai and Tang, Liping
Journal: Acta biomaterialia (2020): 95-104

Pre-targeted Imaging of Protease Activity through In Situ Assembly of Nanoparticles.
Authors: Chen, Zixin and Chen, Min and Zhou, Kaixiang and Rao, Jianghong
Journal: Angewandte Chemie (International ed. in English) (2020): 7864-7870

Amplite 比色法Bradford蛋白定量分析试剂盒

简要概述

传统的Bradford蛋白质检测法广泛用于定量蛋白质浓度。但是，许多市场的许多操作说明非常复杂繁琐。 我们的Amplite 比色法Braford蛋白定量检测试剂盒是一种用于测定总蛋白浓度的包含两组分且与洗涤剂兼容的测定方法。该检测法基于与Bradford蛋白质测定法相同的考马斯亮蓝G-250蛋白指示剂，并提供相当的准确性。我们专有的配方使我们的试剂盒更加方便快捷。在600 nm附近检测蛋白质信号，并在30分钟内完成测定。 Amplite 比色Braford蛋白定量分析试剂盒可以以方便的96孔板进行操作，无需分离步骤即可轻松实现自动化。

产品说明书

样品分析方案

概述

标准溶液制备：

BSA标准溶液：

将20 µL的1 mg / mL BSA标准溶液（组分C）添加到480 µL的PBS（未提供）中，以生成40 µg / mL的BSA标准溶液（BS1）。然后在PBS中执行1：2连续稀释，以得到连续稀释的BSA标准BS2-BS7。注意：有必要为每个测定创建标准曲线。

操作步骤

表1.在透明的底96孔板中布置BSA标准品和测试样品。 BS = BSA标准品（BS1-BS7，40至0.625 µg / mL）; BL =空白控件； TS =测试样品

图示

图1.使用透明底96孔板，使用Amplite 比色法Bradford蛋白定量分析试剂盒测量BSA剂量响应。

参考文献

The Effects of a Concentrated Surfactant Gel on Biofilm EPS.
Authors: Salisbury, Anne-Marie and Chen, Rui and Mullin, Marc and Foulkes, Lauren and Percival, Steven L
Journal: Surgical technology international (2020): 31-35

The effect of Chinese wild blueberry fractions on the growth and membrane integrity of various foodborne pathogens.
Authors: Zhou, Tong-Tong and Wei, Cai-Hong and Lan, Wei-Qing and Zhao, Yong and Pan, Ying-Jie and Sun, Xiao-Hong and Wu, Vivian C H
Journal: Journal of food science (2020): 1513-1522

[Preliminary Investigation on Difference of Protein Compositions Between DC2.4 Cells and Their Derived Exosomes by nanoLC-MS/MS].
Authors: Lin, Qing and Li, Yan and Qu, Meng-Ke and Liu, Xing and Zhang, Zhi-Rong and Zhang, Ling
Journal: Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition (2020): 81-86

Augmented COlorimetric NANoplasmonic (CONAN) Method for Grading Purity and Determine Concentration of EV Microliter Volume Solutions.
Authors: Zendrini, Andrea and Paolini, Lucia and Busatto, Sara and Radeghieri, Annalisa and Romano, Miriam and Wauben, Marca H M and van Herwijnen, Martijn J C and Nejsum, Peter and Borup, Anne and Ridolfi, Andrea and Montis, Costanza and Bergese, Paolo
Journal: Frontiers in bioengineering and biotechnology (2019): 452

Combined Fluorimetric Caspase-3/7 Assay and Bradford Protein Determination for Assessment of Polycation-Mediated Cytotoxicity.
Authors: Larsen, Anna K and Hall, Arnaldur and Lundsgart, Henrik and Moghimi, S Moein
Journal: Methods in molecular biology (Clifton, N.J.) (2019): 301-311

Effect of nanoparticle size and PEGylation on the protein corona of PLGA nanoparticles.
Authors: Partikel, Katrin and Korte, Robin and Stein, Nora C and Mulac, Dennis and Herrmann, Fabian C and Humpf, Hans-Ulrich and Langer, Klaus
Journal: European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V (2019): 70-80

Magnetic Nanoclusters Coated with Albumin, Casein, and Gelatin: Size Tuning, Relaxivity, Stability, Protein Corona, and Application in Nuclear Magnetic Resonance Immunoassay.
Authors: Khramtsov, Pavel and Barkina, Irina and Kropaneva, Maria and Bochkova, Maria and Timganova, Valeria and Nechaev, Anton and Byzov, Il’ya and Zamorina, Svetlana and Yermakov, Anatoly and Rayev, Mikhail
Journal: Nanomaterials (Basel, Switzerland) (2019)

Pilose Antler Extracts (PAEs) Protect against Neurodegeneration in 6-OHDA-Induced Parkinson’s Disease Rat Models.
Authors: Li, Chaohua and Sun, Yanan and Yang, Weifeng and Ma, Shuhua and Zhang, Lili and Zhao, Jing and Zhao, Xin and Wang, Yi
Journal: Evidence-based complementary and alternative medicine : eCAM (2019): 7276407

Rhubarb-Evoke Mucus Secretion through Aggregation and Degranulation of Mast Cell in the Colon of Rat: In vivo and ex vivo studies.
Authors: Wu, Di and Xue, Xiaowei and Gao, Chenchen and Liu, Yuehong and Wang, Tiantian and Li, Lisheng and Tong, Xuehong and Li, Feng and Xu, Jingdong
Journal: Scientific reports (2019): 19375

Serum type and concentration both affect the protein-corona composition of PLGA nanoparticles.
Authors: Partikel, Katrin and Korte, Robin and Mulac, Dennis and Humpf, Hans-Ulrich and Langer, Klaus
Journal: Beilstein journal of nanotechnology (2019): 1002-1015